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Silencing of insulin-like growth factor-1 receptor enhances the radiation sensitivity of human esophageal squamous cell carcinoma in vitro and in vivo.

Zhao H, Gu X - World J Surg Oncol (2014)

Bottom Line: Eca-109 and TE-1 cells were transfected with 100 nM IGF-1r siRNA, and a combination of IGF-1r siRNA and radiation therapy was tested in vitro and in vivo.The effects of IGF-1r siRNA were determined through Western blotting and flow cytometry experiments.In addition, the Eca-109 and TE-1 cells that were irradiated following IGF-1r knockdown contained 16.2% and 20.3% apoptotic cells, respectively.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology, Qilu Hospital of Shandong University, Wenhua Western Road 107, Jinan 250012 Shandong Province, China. guxiaomengff@126.com.

ABSTRACT

Background: Esophageal squamous cell carcinoma (ESCC) is a prevalent fatal cancer worldwide, and the number of deaths due to this disease is increasing. Due to ESCC resistance to chemotherapy and radiation treatment, new therapies are urgently needed for the improvement of ESCC patient clinical outcomes.

Methods: Eca-109 and TE-1 cells were transfected with 100 nM IGF-1r siRNA, and a combination of IGF-1r siRNA and radiation therapy was tested in vitro and in vivo. The effects of IGF-1r siRNA were determined through Western blotting and flow cytometry experiments.

Results: After radiotherapy, the number of IGF-1r siRNA-transfected Eca-109 cells decreased by approximately 67.3%, and a 78.9% reduction was observed in the transfected TE-1 cells. In addition, the Eca-109 and TE-1 cells that were irradiated following IGF-1r knockdown contained 16.2% and 20.3% apoptotic cells, respectively.

Conclusions: The results of the current study suggest that IGF-1r knockdown may enhance the radiation sensitivity of ESCC and increase the therapeutic effects of radiation both in vitro and in vivo. These results provide strong evidence that the targeted application of siRNA will enable the development of new therapeutic strategies for the clinical treatment of ESCC patients.

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Transfection efficiency ofIGF-1rsiRNAin vitro. (A)IGF-1r protein expression was analyzed by Western blotting after transfection with IGF-1r siRNA for 72 hours in TE-1 and Eca-109 cells. (B) The transfection efficiency of IGF-1r siRNA was observed by fluorescence microscopy after 72 hours in TE-1 and Eca-109 cells. β-actin was used as a loading control.
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Fig1: Transfection efficiency ofIGF-1rsiRNAin vitro. (A)IGF-1r protein expression was analyzed by Western blotting after transfection with IGF-1r siRNA for 72 hours in TE-1 and Eca-109 cells. (B) The transfection efficiency of IGF-1r siRNA was observed by fluorescence microscopy after 72 hours in TE-1 and Eca-109 cells. β-actin was used as a loading control.

Mentions: Western blotting was used to test the expression level of IGF-1r to evaluate the transfection efficiency of the IGF-1r siRNA. It was confirmed that the expression of IGF-1r decreased by approximately 33% and 46% in the Eca-109 and TE-1 cells, respectively, after IGF-1r siRNA transfection for 72 hours. In addition, a difference in IGF-1r expression between Eca-109 and TE-1 cells was also observed, with IGF-1r expression being much higher in TE-1 cells than in Eca-109 cells (Figure 1A).Figure 1


Silencing of insulin-like growth factor-1 receptor enhances the radiation sensitivity of human esophageal squamous cell carcinoma in vitro and in vivo.

Zhao H, Gu X - World J Surg Oncol (2014)

Transfection efficiency ofIGF-1rsiRNAin vitro. (A)IGF-1r protein expression was analyzed by Western blotting after transfection with IGF-1r siRNA for 72 hours in TE-1 and Eca-109 cells. (B) The transfection efficiency of IGF-1r siRNA was observed by fluorescence microscopy after 72 hours in TE-1 and Eca-109 cells. β-actin was used as a loading control.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4232704&req=5

Fig1: Transfection efficiency ofIGF-1rsiRNAin vitro. (A)IGF-1r protein expression was analyzed by Western blotting after transfection with IGF-1r siRNA for 72 hours in TE-1 and Eca-109 cells. (B) The transfection efficiency of IGF-1r siRNA was observed by fluorescence microscopy after 72 hours in TE-1 and Eca-109 cells. β-actin was used as a loading control.
Mentions: Western blotting was used to test the expression level of IGF-1r to evaluate the transfection efficiency of the IGF-1r siRNA. It was confirmed that the expression of IGF-1r decreased by approximately 33% and 46% in the Eca-109 and TE-1 cells, respectively, after IGF-1r siRNA transfection for 72 hours. In addition, a difference in IGF-1r expression between Eca-109 and TE-1 cells was also observed, with IGF-1r expression being much higher in TE-1 cells than in Eca-109 cells (Figure 1A).Figure 1

Bottom Line: Eca-109 and TE-1 cells were transfected with 100 nM IGF-1r siRNA, and a combination of IGF-1r siRNA and radiation therapy was tested in vitro and in vivo.The effects of IGF-1r siRNA were determined through Western blotting and flow cytometry experiments.In addition, the Eca-109 and TE-1 cells that were irradiated following IGF-1r knockdown contained 16.2% and 20.3% apoptotic cells, respectively.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology, Qilu Hospital of Shandong University, Wenhua Western Road 107, Jinan 250012 Shandong Province, China. guxiaomengff@126.com.

ABSTRACT

Background: Esophageal squamous cell carcinoma (ESCC) is a prevalent fatal cancer worldwide, and the number of deaths due to this disease is increasing. Due to ESCC resistance to chemotherapy and radiation treatment, new therapies are urgently needed for the improvement of ESCC patient clinical outcomes.

Methods: Eca-109 and TE-1 cells were transfected with 100 nM IGF-1r siRNA, and a combination of IGF-1r siRNA and radiation therapy was tested in vitro and in vivo. The effects of IGF-1r siRNA were determined through Western blotting and flow cytometry experiments.

Results: After radiotherapy, the number of IGF-1r siRNA-transfected Eca-109 cells decreased by approximately 67.3%, and a 78.9% reduction was observed in the transfected TE-1 cells. In addition, the Eca-109 and TE-1 cells that were irradiated following IGF-1r knockdown contained 16.2% and 20.3% apoptotic cells, respectively.

Conclusions: The results of the current study suggest that IGF-1r knockdown may enhance the radiation sensitivity of ESCC and increase the therapeutic effects of radiation both in vitro and in vivo. These results provide strong evidence that the targeted application of siRNA will enable the development of new therapeutic strategies for the clinical treatment of ESCC patients.

Show MeSH
Related in: MedlinePlus