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Recent advances in hydrogen peroxide imaging for biological applications.

Guo H, Aleyasin H, Dickinson BC, Haskew-Layton RE, Ratan RR - Cell Biosci (2014)

Bottom Line: Therefore, the direct measurement of H2O2 in living specimens is critically important.Advances in H2O2 measurement have enabled biomedical scientists to study H2O2 biology at a level of precision previously unachievable.In addition, new imaging techniques such as two-photon microscopy (TPM) have been employed for H2O2 detection, which permit real-time measurements of H2O2 in vivo.

View Article: PubMed Central - PubMed

Affiliation: Fischell Department of Bioengineering, University of Maryland, College Park, MD 20742 USA ; Burke Medical Research Institute, Weill Medical College of Cornell University, White Plains, NY 10605 USA.

ABSTRACT
Mounting evidence supports the role of hydrogen peroxide (H2O2) in physiological signaling as well as pathological conditions. However, the subtleties of peroxide-mediated signaling are not well understood, in part because the generation, degradation, and diffusion of H2O2 are highly volatile within different cellular compartments. Therefore, the direct measurement of H2O2 in living specimens is critically important. Fluorescent probes that can detect small changes in H2O2 levels within relevant cellular compartments are important tools to study the spatial dynamics of H2O2. To achieve temporal resolution, the probes must also be photostable enough to allow multiple readings over time without loss of signal. Traditional fluorescent redox sensitive probes that have been commonly used for the detection of H2O2 tend to react with a wide variety of reactive oxygen species (ROS) and often suffer from photostablilty issues. Recently, new classes of H2O2 probes have been designed to detect H2O2 with high selectivity. Advances in H2O2 measurement have enabled biomedical scientists to study H2O2 biology at a level of precision previously unachievable. In addition, new imaging techniques such as two-photon microscopy (TPM) have been employed for H2O2 detection, which permit real-time measurements of H2O2 in vivo. This review focuses on recent advances in H2O2 probe development and optical imaging technologies that have been developed for biomedical applications.

No MeSH data available.


Related in: MedlinePlus

Ratiometric imaging of fresh rat hippocampal slice treated with H2O2. (A) The reaction between PN1 and H2O2 produced AN1 as the only major fluorescent product. (B) A hippocampal slice labeled with PN1. (C) Fluorescence spectra responses of 3 μM PN1 to 1 mM H2O2. Spectra were acquired at 0, 10, 20, 30, 40, 50, 60, and 120 min after H2O2 was added. (D) A hippocampal slice labeled with PN1 after pretreated with H2O2. Scale bars: 30 μm. The figures were adapted from ref. [50] with permission.
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Fig3: Ratiometric imaging of fresh rat hippocampal slice treated with H2O2. (A) The reaction between PN1 and H2O2 produced AN1 as the only major fluorescent product. (B) A hippocampal slice labeled with PN1. (C) Fluorescence spectra responses of 3 μM PN1 to 1 mM H2O2. Spectra were acquired at 0, 10, 20, 30, 40, 50, 60, and 120 min after H2O2 was added. (D) A hippocampal slice labeled with PN1 after pretreated with H2O2. Scale bars: 30 μm. The figures were adapted from ref. [50] with permission.

Mentions: Figure 3 shows TPM ratiometric image of a fresh rat hippocampal slice treated with H2O2 production. This imaging technique provides a solution for deep tissues H2O2 quantitative analysis.Figure 3


Recent advances in hydrogen peroxide imaging for biological applications.

Guo H, Aleyasin H, Dickinson BC, Haskew-Layton RE, Ratan RR - Cell Biosci (2014)

Ratiometric imaging of fresh rat hippocampal slice treated with H2O2. (A) The reaction between PN1 and H2O2 produced AN1 as the only major fluorescent product. (B) A hippocampal slice labeled with PN1. (C) Fluorescence spectra responses of 3 μM PN1 to 1 mM H2O2. Spectra were acquired at 0, 10, 20, 30, 40, 50, 60, and 120 min after H2O2 was added. (D) A hippocampal slice labeled with PN1 after pretreated with H2O2. Scale bars: 30 μm. The figures were adapted from ref. [50] with permission.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4232666&req=5

Fig3: Ratiometric imaging of fresh rat hippocampal slice treated with H2O2. (A) The reaction between PN1 and H2O2 produced AN1 as the only major fluorescent product. (B) A hippocampal slice labeled with PN1. (C) Fluorescence spectra responses of 3 μM PN1 to 1 mM H2O2. Spectra were acquired at 0, 10, 20, 30, 40, 50, 60, and 120 min after H2O2 was added. (D) A hippocampal slice labeled with PN1 after pretreated with H2O2. Scale bars: 30 μm. The figures were adapted from ref. [50] with permission.
Mentions: Figure 3 shows TPM ratiometric image of a fresh rat hippocampal slice treated with H2O2 production. This imaging technique provides a solution for deep tissues H2O2 quantitative analysis.Figure 3

Bottom Line: Therefore, the direct measurement of H2O2 in living specimens is critically important.Advances in H2O2 measurement have enabled biomedical scientists to study H2O2 biology at a level of precision previously unachievable.In addition, new imaging techniques such as two-photon microscopy (TPM) have been employed for H2O2 detection, which permit real-time measurements of H2O2 in vivo.

View Article: PubMed Central - PubMed

Affiliation: Fischell Department of Bioengineering, University of Maryland, College Park, MD 20742 USA ; Burke Medical Research Institute, Weill Medical College of Cornell University, White Plains, NY 10605 USA.

ABSTRACT
Mounting evidence supports the role of hydrogen peroxide (H2O2) in physiological signaling as well as pathological conditions. However, the subtleties of peroxide-mediated signaling are not well understood, in part because the generation, degradation, and diffusion of H2O2 are highly volatile within different cellular compartments. Therefore, the direct measurement of H2O2 in living specimens is critically important. Fluorescent probes that can detect small changes in H2O2 levels within relevant cellular compartments are important tools to study the spatial dynamics of H2O2. To achieve temporal resolution, the probes must also be photostable enough to allow multiple readings over time without loss of signal. Traditional fluorescent redox sensitive probes that have been commonly used for the detection of H2O2 tend to react with a wide variety of reactive oxygen species (ROS) and often suffer from photostablilty issues. Recently, new classes of H2O2 probes have been designed to detect H2O2 with high selectivity. Advances in H2O2 measurement have enabled biomedical scientists to study H2O2 biology at a level of precision previously unachievable. In addition, new imaging techniques such as two-photon microscopy (TPM) have been employed for H2O2 detection, which permit real-time measurements of H2O2 in vivo. This review focuses on recent advances in H2O2 probe development and optical imaging technologies that have been developed for biomedical applications.

No MeSH data available.


Related in: MedlinePlus