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Effect of germinated brown rice extracts on pancreatic lipase, adipogenesis and lipolysis in 3T3-L1 adipocytes.

Lim SM, Goh YM, Kuan WB, Loh SP - Lipids Health Dis (2014)

Bottom Line: GBR extract using hexane showed the highest inhibitory effect (13.58 ± 0.860%) at concentration of 200 μg/ml followed by hexane extract at 100 μg/ml (9.98 ± 1.048%) while ethyl acetate extract showed the lowest (2.62 ± 0.677%) at concentration of 200 μg/ml on pancreatic lipase activity.It significantly decrease (p < 0.05) lipid accumulation than control (OROSM = 100%), follow by ethyl acetate extract at 300 μg/ml (OROSM = 65.17 ± 3.131%).GBR extracts especially the least polar and intermediate polar solvent extracts exhibited inhibitory effect on pancreatic lipase, decrease fat accumulation by adipocyte differentiation inhibition, and stimulate lipolysis on adipocytes.

View Article: PubMed Central - PubMed

Affiliation: Department of Nutrition and Dietetics, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia. sploh@upm.edu.my.

ABSTRACT

Background: This study investigated anti-obesity effects of seven different solvent (n-hexane, toluene, dicholoromethane, ethyl acetate, absolute methanol, 80% methanol and deionized water) extracts of germinated brown rice (GBR) on pancreatic lipase activity, adipogenesis and lipolysis in 3T3-L1 adipocytes.

Methods: GBR were extracted separately by employing different solvents with ultrasound-assisted. Pancreatic lipase activity was determined spectrophotometrically by measuring the hydrolysis of p-nitrophenyl butyrate (p-NPB) to p-nitrophenol at 405 nm. Adipogenesis and lipolysis were assayed in fully differentiated 3T3-L1 adipocytes by using Oil Red O staining and glycerol release measurement.

Results: GBR extract using hexane showed the highest inhibitory effect (13.58 ± 0.860%) at concentration of 200 μg/ml followed by hexane extract at 100 μg/ml (9.98 ± 1.048%) while ethyl acetate extract showed the lowest (2.62 ± 0.677%) at concentration of 200 μg/ml on pancreatic lipase activity. Water extract at 300 μg/ml showed 61.55 ± 3.824% of Oil Red O staining material (OROSM), a marker of adipogenesis. It significantly decrease (p < 0.05) lipid accumulation than control (OROSM = 100%), follow by ethyl acetate extract at 300 μg/ml (OROSM = 65.17 ± 3.131%). All the GBR extracts induced lipolysis with 1.22-1.83 fold of greater glycerol release than control.

Conclusions: GBR extracts especially the least polar and intermediate polar solvent extracts exhibited inhibitory effect on pancreatic lipase, decrease fat accumulation by adipocyte differentiation inhibition, and stimulate lipolysis on adipocytes. Therefore, GBR could be furthered study and developed as a functional food in helping the treatment and/or prevention of obesity.

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Related in: MedlinePlus

Extent of lipolysis based on the amount of glycerol released across different GBR extracts. Results are given as a mean value ± S.D. of six replicate measurements. Bar graphs represent the relative glycerol release content of 3T3-L1 adipocytes after treated with various GBR extracts in different concentrations. a-dValues with different lower case letters are significantly different at p < 0.05.
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Fig3: Extent of lipolysis based on the amount of glycerol released across different GBR extracts. Results are given as a mean value ± S.D. of six replicate measurements. Bar graphs represent the relative glycerol release content of 3T3-L1 adipocytes after treated with various GBR extracts in different concentrations. a-dValues with different lower case letters are significantly different at p < 0.05.

Mentions: Lipolysis is a catabolic process that hydrolyzes stored triglycerides in adipose tissue to release free fatty acid and glycerol. Thus, lipolysis of fat cells can regulate the homeostasis of energy by controlling the release of fatty acids and glycerol into plasma[55]. The lipolytic effect of GBR extracts was investigated through the measurement of glycerol released in culture medium after 24 h incubation, as shown in Figure 3. All the GBR extracts stimulated mild lipolysis by induced 1.22-1.83 fold greater release of glycerol into the culture medium than control. Toluene extract at concentration of 300 μg/ml showed the highest glycerol release content of adipocytes with 1.83 fold of increase and followed by hexane extract at concentration of 200 μg/ml (1.76 fold). Absolute methanol extract at concentration of 200 μg/ml showed the lowest glycerol release content of adipocytes with 1.22 fold of increase than control.Figure 3


Effect of germinated brown rice extracts on pancreatic lipase, adipogenesis and lipolysis in 3T3-L1 adipocytes.

Lim SM, Goh YM, Kuan WB, Loh SP - Lipids Health Dis (2014)

Extent of lipolysis based on the amount of glycerol released across different GBR extracts. Results are given as a mean value ± S.D. of six replicate measurements. Bar graphs represent the relative glycerol release content of 3T3-L1 adipocytes after treated with various GBR extracts in different concentrations. a-dValues with different lower case letters are significantly different at p < 0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4232653&req=5

Fig3: Extent of lipolysis based on the amount of glycerol released across different GBR extracts. Results are given as a mean value ± S.D. of six replicate measurements. Bar graphs represent the relative glycerol release content of 3T3-L1 adipocytes after treated with various GBR extracts in different concentrations. a-dValues with different lower case letters are significantly different at p < 0.05.
Mentions: Lipolysis is a catabolic process that hydrolyzes stored triglycerides in adipose tissue to release free fatty acid and glycerol. Thus, lipolysis of fat cells can regulate the homeostasis of energy by controlling the release of fatty acids and glycerol into plasma[55]. The lipolytic effect of GBR extracts was investigated through the measurement of glycerol released in culture medium after 24 h incubation, as shown in Figure 3. All the GBR extracts stimulated mild lipolysis by induced 1.22-1.83 fold greater release of glycerol into the culture medium than control. Toluene extract at concentration of 300 μg/ml showed the highest glycerol release content of adipocytes with 1.83 fold of increase and followed by hexane extract at concentration of 200 μg/ml (1.76 fold). Absolute methanol extract at concentration of 200 μg/ml showed the lowest glycerol release content of adipocytes with 1.22 fold of increase than control.Figure 3

Bottom Line: GBR extract using hexane showed the highest inhibitory effect (13.58 ± 0.860%) at concentration of 200 μg/ml followed by hexane extract at 100 μg/ml (9.98 ± 1.048%) while ethyl acetate extract showed the lowest (2.62 ± 0.677%) at concentration of 200 μg/ml on pancreatic lipase activity.It significantly decrease (p < 0.05) lipid accumulation than control (OROSM = 100%), follow by ethyl acetate extract at 300 μg/ml (OROSM = 65.17 ± 3.131%).GBR extracts especially the least polar and intermediate polar solvent extracts exhibited inhibitory effect on pancreatic lipase, decrease fat accumulation by adipocyte differentiation inhibition, and stimulate lipolysis on adipocytes.

View Article: PubMed Central - PubMed

Affiliation: Department of Nutrition and Dietetics, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia. sploh@upm.edu.my.

ABSTRACT

Background: This study investigated anti-obesity effects of seven different solvent (n-hexane, toluene, dicholoromethane, ethyl acetate, absolute methanol, 80% methanol and deionized water) extracts of germinated brown rice (GBR) on pancreatic lipase activity, adipogenesis and lipolysis in 3T3-L1 adipocytes.

Methods: GBR were extracted separately by employing different solvents with ultrasound-assisted. Pancreatic lipase activity was determined spectrophotometrically by measuring the hydrolysis of p-nitrophenyl butyrate (p-NPB) to p-nitrophenol at 405 nm. Adipogenesis and lipolysis were assayed in fully differentiated 3T3-L1 adipocytes by using Oil Red O staining and glycerol release measurement.

Results: GBR extract using hexane showed the highest inhibitory effect (13.58 ± 0.860%) at concentration of 200 μg/ml followed by hexane extract at 100 μg/ml (9.98 ± 1.048%) while ethyl acetate extract showed the lowest (2.62 ± 0.677%) at concentration of 200 μg/ml on pancreatic lipase activity. Water extract at 300 μg/ml showed 61.55 ± 3.824% of Oil Red O staining material (OROSM), a marker of adipogenesis. It significantly decrease (p < 0.05) lipid accumulation than control (OROSM = 100%), follow by ethyl acetate extract at 300 μg/ml (OROSM = 65.17 ± 3.131%). All the GBR extracts induced lipolysis with 1.22-1.83 fold of greater glycerol release than control.

Conclusions: GBR extracts especially the least polar and intermediate polar solvent extracts exhibited inhibitory effect on pancreatic lipase, decrease fat accumulation by adipocyte differentiation inhibition, and stimulate lipolysis on adipocytes. Therefore, GBR could be furthered study and developed as a functional food in helping the treatment and/or prevention of obesity.

Show MeSH
Related in: MedlinePlus