Limits...
An ER-associated miRNA signature predicts prognosis in ER-positive breast cancer.

Zhou X, Wang X, Huang Z, Xu L, Zhu W, Liu P - J. Exp. Clin. Cancer Res. (2014)

Bottom Line: A total of 14 miRNAs were observed to be associated with the status of ER by significance analysis of microarrays (SAM) in the training set.Patients were characterized as high score or low score group according to the calculated risk scores from each miRNA.And patients in high score group had worse overall survival compared with those in low score group both in the training and testing set.

View Article: PubMed Central - PubMed

Affiliation: Department of Oncology, First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing, 210029, China. ivorchou@yeah.net.

ABSTRACT

Background: Breast cancer patients with positive estrogen receptor (ER) have a better prognosis. However, no prognostic miRNA signature was reported in the ER-positive breast cancer. The aim of the study was to identify and assess the prognostic significance of a miRNA signature in ER-positive breast cancer.

Methods: Two cohorts from The Cancer Genome Atlas (TCGA) dataset were used as training (n =596) and testing set (n =319). Differential expression profiling was identified in the training set. And the prognostic value of the miRNA signature was then assessed in the two cohorts.

Results: A total of 14 miRNAs were observed to be associated with the status of ER by significance analysis of microarrays (SAM) in the training set. Patients were characterized as high score or low score group according to the calculated risk scores from each miRNA. And patients in high score group had worse overall survival compared with those in low score group both in the training and testing set.

Conclusions: Our study revealed a miRNA signature including 14 miRNAs associated with ER status which could act as a prognostic marker in ER-positive breast cancer.

Show MeSH

Related in: MedlinePlus

Risk scores for the ER-associated miRNA signature and outcome in breast cancer patients with positive ER status. (A) Training set and (C) validation set: (Top) survival status and duration of cases; (Middle) risk scores of miRNA signature; (Bottom) low and high score group for the 14 miRNAs; Kaplan-Meier curves for the low score and high score groups in both training set (B) and validation set (D). Patients with high score had worse overall survival than those with low score.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC4232612&req=5

Fig3: Risk scores for the ER-associated miRNA signature and outcome in breast cancer patients with positive ER status. (A) Training set and (C) validation set: (Top) survival status and duration of cases; (Middle) risk scores of miRNA signature; (Bottom) low and high score group for the 14 miRNAs; Kaplan-Meier curves for the low score and high score groups in both training set (B) and validation set (D). Patients with high score had worse overall survival than those with low score.

Mentions: By combining cases from the two cohorts, better overall survival could be found (Figure 2) in the ER-positive patients compared with ER-negative cases (P = 0.019). To assess the prognostic value of ER associated miRNA signature, 456 ER-positive cases in training set was divided into high and low score group according to the median risk score (ROC curves for each miRNA were present in the Additional file 1: Figure S1). As shown in Figure 3A, the up-regulated miRNAs identified in ER-negative cases exhibit high expression in high score group and the down-regulated miRNAs have high expression in low score group. And the patients with high score tended to have poor overall survival. Kaplan-Meier curves for the two groups were shown in Figure 3B. ER-positive patients in high score group suffered worse overall survival than those in low score group (P = 0.022).Figure 2


An ER-associated miRNA signature predicts prognosis in ER-positive breast cancer.

Zhou X, Wang X, Huang Z, Xu L, Zhu W, Liu P - J. Exp. Clin. Cancer Res. (2014)

Risk scores for the ER-associated miRNA signature and outcome in breast cancer patients with positive ER status. (A) Training set and (C) validation set: (Top) survival status and duration of cases; (Middle) risk scores of miRNA signature; (Bottom) low and high score group for the 14 miRNAs; Kaplan-Meier curves for the low score and high score groups in both training set (B) and validation set (D). Patients with high score had worse overall survival than those with low score.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4232612&req=5

Fig3: Risk scores for the ER-associated miRNA signature and outcome in breast cancer patients with positive ER status. (A) Training set and (C) validation set: (Top) survival status and duration of cases; (Middle) risk scores of miRNA signature; (Bottom) low and high score group for the 14 miRNAs; Kaplan-Meier curves for the low score and high score groups in both training set (B) and validation set (D). Patients with high score had worse overall survival than those with low score.
Mentions: By combining cases from the two cohorts, better overall survival could be found (Figure 2) in the ER-positive patients compared with ER-negative cases (P = 0.019). To assess the prognostic value of ER associated miRNA signature, 456 ER-positive cases in training set was divided into high and low score group according to the median risk score (ROC curves for each miRNA were present in the Additional file 1: Figure S1). As shown in Figure 3A, the up-regulated miRNAs identified in ER-negative cases exhibit high expression in high score group and the down-regulated miRNAs have high expression in low score group. And the patients with high score tended to have poor overall survival. Kaplan-Meier curves for the two groups were shown in Figure 3B. ER-positive patients in high score group suffered worse overall survival than those in low score group (P = 0.022).Figure 2

Bottom Line: A total of 14 miRNAs were observed to be associated with the status of ER by significance analysis of microarrays (SAM) in the training set.Patients were characterized as high score or low score group according to the calculated risk scores from each miRNA.And patients in high score group had worse overall survival compared with those in low score group both in the training and testing set.

View Article: PubMed Central - PubMed

Affiliation: Department of Oncology, First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing, 210029, China. ivorchou@yeah.net.

ABSTRACT

Background: Breast cancer patients with positive estrogen receptor (ER) have a better prognosis. However, no prognostic miRNA signature was reported in the ER-positive breast cancer. The aim of the study was to identify and assess the prognostic significance of a miRNA signature in ER-positive breast cancer.

Methods: Two cohorts from The Cancer Genome Atlas (TCGA) dataset were used as training (n =596) and testing set (n =319). Differential expression profiling was identified in the training set. And the prognostic value of the miRNA signature was then assessed in the two cohorts.

Results: A total of 14 miRNAs were observed to be associated with the status of ER by significance analysis of microarrays (SAM) in the training set. Patients were characterized as high score or low score group according to the calculated risk scores from each miRNA. And patients in high score group had worse overall survival compared with those in low score group both in the training and testing set.

Conclusions: Our study revealed a miRNA signature including 14 miRNAs associated with ER status which could act as a prognostic marker in ER-positive breast cancer.

Show MeSH
Related in: MedlinePlus