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Potential conservation of circadian clock proteins in the phylum Nematoda as revealed by bioinformatic searches.

Romanowski A, Garavaglia MJ, Goya ME, Ghiringhelli PD, Golombek DA - PLoS ONE (2014)

Bottom Line: Although several circadian rhythms have been described in C. elegans, its molecular clock remains elusive.In this work we employed a novel bioinformatic approach, applying probabilistic methodologies, to search for circadian clock proteins of several of the best studied circadian model organisms of different taxa (Mus musculus, Drosophila melanogaster, Neurospora crassa, Arabidopsis thaliana and Synechoccocus elongatus) in the proteomes of C. elegans and other members of the phylum Nematoda.With this approach we found that the Nematoda contain proteins most related to the core and accessory proteins of the insect and mammalian clocks, which provide new insights into the nematode clock and the evolution of the circadian system.

View Article: PubMed Central - PubMed

Affiliation: Laboratorio de Cronobiología, Dto de Ciencia y Tecnología, Universidad Nacional de Quilmes, Bernal, Buenos Aires, Argentina.

ABSTRACT
Although several circadian rhythms have been described in C. elegans, its molecular clock remains elusive. In this work we employed a novel bioinformatic approach, applying probabilistic methodologies, to search for circadian clock proteins of several of the best studied circadian model organisms of different taxa (Mus musculus, Drosophila melanogaster, Neurospora crassa, Arabidopsis thaliana and Synechoccocus elongatus) in the proteomes of C. elegans and other members of the phylum Nematoda. With this approach we found that the Nematoda contain proteins most related to the core and accessory proteins of the insect and mammalian clocks, which provide new insights into the nematode clock and the evolution of the circadian system.

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Methodology workflow.The diagram briefly describes the methods we applied in this work.
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pone-0112871-g001: Methodology workflow.The diagram briefly describes the methods we applied in this work.

Mentions: Using the workflow depicted in figure 1, we first analyzed the existence of clock components similar to those from the most well-known circadian model organisms of 5 different phyla: Arabidopsis thaliana (plant), Synechococcus elongatus (cyanobacteria), Neurospora crassa (fungi), Drosophila melanogaster (insect), Mus musculus (mammal), in C. elegans and then the corresponding ortholog proteins in the rest of the nematodes used in this work.


Potential conservation of circadian clock proteins in the phylum Nematoda as revealed by bioinformatic searches.

Romanowski A, Garavaglia MJ, Goya ME, Ghiringhelli PD, Golombek DA - PLoS ONE (2014)

Methodology workflow.The diagram briefly describes the methods we applied in this work.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4232591&req=5

pone-0112871-g001: Methodology workflow.The diagram briefly describes the methods we applied in this work.
Mentions: Using the workflow depicted in figure 1, we first analyzed the existence of clock components similar to those from the most well-known circadian model organisms of 5 different phyla: Arabidopsis thaliana (plant), Synechococcus elongatus (cyanobacteria), Neurospora crassa (fungi), Drosophila melanogaster (insect), Mus musculus (mammal), in C. elegans and then the corresponding ortholog proteins in the rest of the nematodes used in this work.

Bottom Line: Although several circadian rhythms have been described in C. elegans, its molecular clock remains elusive.In this work we employed a novel bioinformatic approach, applying probabilistic methodologies, to search for circadian clock proteins of several of the best studied circadian model organisms of different taxa (Mus musculus, Drosophila melanogaster, Neurospora crassa, Arabidopsis thaliana and Synechoccocus elongatus) in the proteomes of C. elegans and other members of the phylum Nematoda.With this approach we found that the Nematoda contain proteins most related to the core and accessory proteins of the insect and mammalian clocks, which provide new insights into the nematode clock and the evolution of the circadian system.

View Article: PubMed Central - PubMed

Affiliation: Laboratorio de Cronobiología, Dto de Ciencia y Tecnología, Universidad Nacional de Quilmes, Bernal, Buenos Aires, Argentina.

ABSTRACT
Although several circadian rhythms have been described in C. elegans, its molecular clock remains elusive. In this work we employed a novel bioinformatic approach, applying probabilistic methodologies, to search for circadian clock proteins of several of the best studied circadian model organisms of different taxa (Mus musculus, Drosophila melanogaster, Neurospora crassa, Arabidopsis thaliana and Synechoccocus elongatus) in the proteomes of C. elegans and other members of the phylum Nematoda. With this approach we found that the Nematoda contain proteins most related to the core and accessory proteins of the insect and mammalian clocks, which provide new insights into the nematode clock and the evolution of the circadian system.

Show MeSH