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Magnetic resonance imaging (MRI) to study striatal iron accumulation in a rat model of Parkinson's disease.

Virel A, Faergemann E, Orädd G, Strömberg I - PLoS ONE (2014)

Bottom Line: The results revealed that in comparison with control animals, injection of 6-OHDA into the rat striatum provoked an edematous process, visible in T2-weighted images that was accompanied by an accumulation of iron clearly detectable in T2*-weighted images.Furthermore, Prussian blue staining to detect iron in sectioned brains confirmed the existence of accumulated iron in the areas of T2* hypointensities.The presence of ED1-positive microglia in the lesioned striatum overlapped with this accumulation of iron, indicating areas of toxicity and loss of dopamine nerve fibers.

View Article: PubMed Central - PubMed

Affiliation: Department of Integrative Medical Biology, Umeå University, Umeå, Sweden.

ABSTRACT
Abnormal accumulation of iron is observed in neurodegenerative disorders. In Parkinson's disease, an excess of iron has been demonstrated in different structures of the basal ganglia and is suggested to be involved in the pathogenesis of the disease. Using the 6-hydroxydopamine (6-OHDA) rat model of Parkinson's disease, the edematous effect of 6-OHDA and its relation with striatal iron accumulation was examined utilizing in vivo magnetic resonance imaging (MRI). The results revealed that in comparison with control animals, injection of 6-OHDA into the rat striatum provoked an edematous process, visible in T2-weighted images that was accompanied by an accumulation of iron clearly detectable in T2*-weighted images. Furthermore, Prussian blue staining to detect iron in sectioned brains confirmed the existence of accumulated iron in the areas of T2* hypointensities. The presence of ED1-positive microglia in the lesioned striatum overlapped with this accumulation of iron, indicating areas of toxicity and loss of dopamine nerve fibers. Correlation analyses demonstrated a direct relation between the hyperintensities caused by the edema and the hypointensities caused by the accumulation of iron.

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Immunoglobulin G (IgG) histochemistry.A) control- and B) 6-OHDA injected striatum at 2 days postlesion incubated with anti-rat IgG. Control animals showed almost not reactivity to IgG comparing with lesioned animals indicating that 6-OHDA provokes a disturbed BBB. Scale bar 100 µm.
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pone-0112941-g008: Immunoglobulin G (IgG) histochemistry.A) control- and B) 6-OHDA injected striatum at 2 days postlesion incubated with anti-rat IgG. Control animals showed almost not reactivity to IgG comparing with lesioned animals indicating that 6-OHDA provokes a disturbed BBB. Scale bar 100 µm.

Mentions: The integrity of the BBB was evaluated by studying infiltration of rat IgG in the striatum of control- and 6-OHDA-injected animals (Fig. 8 A and B, respectively). Two days after performing the dopamine lesions an enhanced IgG reactivity was found in the striatum of the lesioned- compared to vehicle-injected animals. In the controls, IgG infiltration was almost absent and only a weak reaction could be observed around the vehicle injection.


Magnetic resonance imaging (MRI) to study striatal iron accumulation in a rat model of Parkinson's disease.

Virel A, Faergemann E, Orädd G, Strömberg I - PLoS ONE (2014)

Immunoglobulin G (IgG) histochemistry.A) control- and B) 6-OHDA injected striatum at 2 days postlesion incubated with anti-rat IgG. Control animals showed almost not reactivity to IgG comparing with lesioned animals indicating that 6-OHDA provokes a disturbed BBB. Scale bar 100 µm.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4232582&req=5

pone-0112941-g008: Immunoglobulin G (IgG) histochemistry.A) control- and B) 6-OHDA injected striatum at 2 days postlesion incubated with anti-rat IgG. Control animals showed almost not reactivity to IgG comparing with lesioned animals indicating that 6-OHDA provokes a disturbed BBB. Scale bar 100 µm.
Mentions: The integrity of the BBB was evaluated by studying infiltration of rat IgG in the striatum of control- and 6-OHDA-injected animals (Fig. 8 A and B, respectively). Two days after performing the dopamine lesions an enhanced IgG reactivity was found in the striatum of the lesioned- compared to vehicle-injected animals. In the controls, IgG infiltration was almost absent and only a weak reaction could be observed around the vehicle injection.

Bottom Line: The results revealed that in comparison with control animals, injection of 6-OHDA into the rat striatum provoked an edematous process, visible in T2-weighted images that was accompanied by an accumulation of iron clearly detectable in T2*-weighted images.Furthermore, Prussian blue staining to detect iron in sectioned brains confirmed the existence of accumulated iron in the areas of T2* hypointensities.The presence of ED1-positive microglia in the lesioned striatum overlapped with this accumulation of iron, indicating areas of toxicity and loss of dopamine nerve fibers.

View Article: PubMed Central - PubMed

Affiliation: Department of Integrative Medical Biology, Umeå University, Umeå, Sweden.

ABSTRACT
Abnormal accumulation of iron is observed in neurodegenerative disorders. In Parkinson's disease, an excess of iron has been demonstrated in different structures of the basal ganglia and is suggested to be involved in the pathogenesis of the disease. Using the 6-hydroxydopamine (6-OHDA) rat model of Parkinson's disease, the edematous effect of 6-OHDA and its relation with striatal iron accumulation was examined utilizing in vivo magnetic resonance imaging (MRI). The results revealed that in comparison with control animals, injection of 6-OHDA into the rat striatum provoked an edematous process, visible in T2-weighted images that was accompanied by an accumulation of iron clearly detectable in T2*-weighted images. Furthermore, Prussian blue staining to detect iron in sectioned brains confirmed the existence of accumulated iron in the areas of T2* hypointensities. The presence of ED1-positive microglia in the lesioned striatum overlapped with this accumulation of iron, indicating areas of toxicity and loss of dopamine nerve fibers. Correlation analyses demonstrated a direct relation between the hyperintensities caused by the edema and the hypointensities caused by the accumulation of iron.

Show MeSH
Related in: MedlinePlus