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Lens subluxation after plasmin and SF6 injections in rabbit eyes.

Wu WC, Liu CH, Wang NK, Chen KJ, Chen TL, Hwang YS, Liao PJ, Li LM, Lai CC - PLoS ONE (2014)

Bottom Line: Histology confirmed zonular fiber damage in the eyes treated with plasmin.Zonular fibers were disrupted following plasmin treatment.These effects should be kept in mind when using plasmin enzymes in patients with vitreoretinal abnormalities.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, Chang Gung Memorial Hospital, Taoyuan, Taiwan; Chang Gung University, College of Medicine, Taoyuan, Taiwan.

ABSTRACT

Purpose: To investigate the rate of lens subluxation following plasmin and/or SF6 injections in eyes, and whether a subsequent elevated level of vascular endothelial growth factor (VEGF) and vitreous tap would aggravate subluxation.

Methods: Four groups of rabbits were used. Group 1 received an intravitreal injection (IVI) of plasmin and SF6 in the right eye; group 2 received an IVI of plasmin in the right eye; group 3 received an IVI of SF6 in the right eye; and group 4 received an IVI of balanced salt solution in the right eye. After treatment, IVIs of VEGF were given and vitreous tap was performed three times, followed by clinical observation of lens subluxation and scanning electronic microscope evaluation of the zonular fibers.

Results: After IVIs of plasmin and SF6, and VEGF and vitreous tap had been performed one to three times, lens subluxation was noted in 0%, 43%, 71%, 71%, and 86% of the eyes in group 1. After IVIs of plasmin, VEGF, and vitreous tap had been performed one to three times, lens subluxation was noted in 11%, 22%, 44%, 44%, and 67% of the eyes in group 2. The eyes in group 3 and 4 did not show signs of lens subluxation after VEGF IVIs and vitreous tap. Histology confirmed zonular fiber damage in the eyes treated with plasmin.

Conclusions: The incidence of lens subluxation increased following plasmin injections in the eyes, and this was aggravated by the subsequent high VEGF level in the eyes and vitreous tapping. Zonular fibers were disrupted following plasmin treatment. These effects should be kept in mind when using plasmin enzymes in patients with vitreoretinal abnormalities.

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Rate of lens subluxation.Rate of lens subluxation after intravitreal injections of plasmin and/or SF6 and subsequent elevation of vascular endothelial growth factor (VEGF) levels, and vitreous tap was performed 3 times is shown. Lens subluxation was noted in the eyes treated with plasmin before the injection of VEGF. After intravitreal injections of VEGF, and vitreous tap was performed 3 times, lens subluxation increased in the eyes treated with plasmin and SF6 (plasmin+SF6 group) or plasmin alone (plasmin group). The eyes treated with SF6 (SF6 group) or BSS (BSS group) showed no signs of lens subluxation.
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pone-0112957-g003: Rate of lens subluxation.Rate of lens subluxation after intravitreal injections of plasmin and/or SF6 and subsequent elevation of vascular endothelial growth factor (VEGF) levels, and vitreous tap was performed 3 times is shown. Lens subluxation was noted in the eyes treated with plasmin before the injection of VEGF. After intravitreal injections of VEGF, and vitreous tap was performed 3 times, lens subluxation increased in the eyes treated with plasmin and SF6 (plasmin+SF6 group) or plasmin alone (plasmin group). The eyes treated with SF6 (SF6 group) or BSS (BSS group) showed no signs of lens subluxation.

Mentions: Lens subluxation was not found in any rabbit (0%; 0/7 eyes) in the plasmin+SF6 group, and one rabbit (11%; 1/9 eyes) in the plasmin group before the intravitreal injection of VEGF. After the intravitreal injection of VEGF, lens subluxation was noted in 43% (3/7) of the eyes in the plasmin+SF6 group and 22% (2/9) of the eyes in the plasmin group. Lens subluxation was not found in the other groups (0/7 and 0/8 for the SF6 and BSS groups, respectively). After the first vitreous tapping, lens subluxation was noted in 71% (5/7) of the eyes in the plasmin+SF6 group and 44% (4/9) of the eyes in the plasmin group. The eyes treated with SF6 (SF6 group) or BSS (BSS group) showed no signs of lens subluxation (0/7 and 0/8 for the SF6 and BSS groups, respectively). After the second vitreous tapping, lens subluxation was noted in 71% (5/7) of the eyes in the plasmin+SF6 group and 44% (4/9) of the eyes in the plasmin group. The eyes treated with SF6 (SF6 group) or BSS (BSS group) still showed no signs of lens subluxation (0/7 and 0/8 for the SF6 and BSS groups, respectively). After the third vitreous tapping, lens subluxation was noted in 86% (6/7) of the eyes in the plasmin+SF6 group and 67% (6/9) of the eyes in the plasmin group. The eyes treated with SF6 (SF6 group) or BSS (BSS group) again showed no signs of lens subluxation (0/7 and 0/8 for the SF6 and BSS groups, respectively). The data are shown in Figure 3. Based on the results of GEE, the degrees of lens subluxation in the plasmin+SF6 and plasmin groups were significantly different from the SF6 group (p<0.0001 and p = 0.035, respectively) and BSS group (p<0.0001 and p = 0.033, respectively). In the analysis of the severity of lens subluxation, 11 eyes (92%) had mild subluxation and 1 eyes (8%) in the plasmin+SF6 group had moderate subluxation out of the 12 eyes with lens subluxation. None of the eyes developed severe subluxation following intravitreal injections.


Lens subluxation after plasmin and SF6 injections in rabbit eyes.

Wu WC, Liu CH, Wang NK, Chen KJ, Chen TL, Hwang YS, Liao PJ, Li LM, Lai CC - PLoS ONE (2014)

Rate of lens subluxation.Rate of lens subluxation after intravitreal injections of plasmin and/or SF6 and subsequent elevation of vascular endothelial growth factor (VEGF) levels, and vitreous tap was performed 3 times is shown. Lens subluxation was noted in the eyes treated with plasmin before the injection of VEGF. After intravitreal injections of VEGF, and vitreous tap was performed 3 times, lens subluxation increased in the eyes treated with plasmin and SF6 (plasmin+SF6 group) or plasmin alone (plasmin group). The eyes treated with SF6 (SF6 group) or BSS (BSS group) showed no signs of lens subluxation.
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Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4232556&req=5

pone-0112957-g003: Rate of lens subluxation.Rate of lens subluxation after intravitreal injections of plasmin and/or SF6 and subsequent elevation of vascular endothelial growth factor (VEGF) levels, and vitreous tap was performed 3 times is shown. Lens subluxation was noted in the eyes treated with plasmin before the injection of VEGF. After intravitreal injections of VEGF, and vitreous tap was performed 3 times, lens subluxation increased in the eyes treated with plasmin and SF6 (plasmin+SF6 group) or plasmin alone (plasmin group). The eyes treated with SF6 (SF6 group) or BSS (BSS group) showed no signs of lens subluxation.
Mentions: Lens subluxation was not found in any rabbit (0%; 0/7 eyes) in the plasmin+SF6 group, and one rabbit (11%; 1/9 eyes) in the plasmin group before the intravitreal injection of VEGF. After the intravitreal injection of VEGF, lens subluxation was noted in 43% (3/7) of the eyes in the plasmin+SF6 group and 22% (2/9) of the eyes in the plasmin group. Lens subluxation was not found in the other groups (0/7 and 0/8 for the SF6 and BSS groups, respectively). After the first vitreous tapping, lens subluxation was noted in 71% (5/7) of the eyes in the plasmin+SF6 group and 44% (4/9) of the eyes in the plasmin group. The eyes treated with SF6 (SF6 group) or BSS (BSS group) showed no signs of lens subluxation (0/7 and 0/8 for the SF6 and BSS groups, respectively). After the second vitreous tapping, lens subluxation was noted in 71% (5/7) of the eyes in the plasmin+SF6 group and 44% (4/9) of the eyes in the plasmin group. The eyes treated with SF6 (SF6 group) or BSS (BSS group) still showed no signs of lens subluxation (0/7 and 0/8 for the SF6 and BSS groups, respectively). After the third vitreous tapping, lens subluxation was noted in 86% (6/7) of the eyes in the plasmin+SF6 group and 67% (6/9) of the eyes in the plasmin group. The eyes treated with SF6 (SF6 group) or BSS (BSS group) again showed no signs of lens subluxation (0/7 and 0/8 for the SF6 and BSS groups, respectively). The data are shown in Figure 3. Based on the results of GEE, the degrees of lens subluxation in the plasmin+SF6 and plasmin groups were significantly different from the SF6 group (p<0.0001 and p = 0.035, respectively) and BSS group (p<0.0001 and p = 0.033, respectively). In the analysis of the severity of lens subluxation, 11 eyes (92%) had mild subluxation and 1 eyes (8%) in the plasmin+SF6 group had moderate subluxation out of the 12 eyes with lens subluxation. None of the eyes developed severe subluxation following intravitreal injections.

Bottom Line: Histology confirmed zonular fiber damage in the eyes treated with plasmin.Zonular fibers were disrupted following plasmin treatment.These effects should be kept in mind when using plasmin enzymes in patients with vitreoretinal abnormalities.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, Chang Gung Memorial Hospital, Taoyuan, Taiwan; Chang Gung University, College of Medicine, Taoyuan, Taiwan.

ABSTRACT

Purpose: To investigate the rate of lens subluxation following plasmin and/or SF6 injections in eyes, and whether a subsequent elevated level of vascular endothelial growth factor (VEGF) and vitreous tap would aggravate subluxation.

Methods: Four groups of rabbits were used. Group 1 received an intravitreal injection (IVI) of plasmin and SF6 in the right eye; group 2 received an IVI of plasmin in the right eye; group 3 received an IVI of SF6 in the right eye; and group 4 received an IVI of balanced salt solution in the right eye. After treatment, IVIs of VEGF were given and vitreous tap was performed three times, followed by clinical observation of lens subluxation and scanning electronic microscope evaluation of the zonular fibers.

Results: After IVIs of plasmin and SF6, and VEGF and vitreous tap had been performed one to three times, lens subluxation was noted in 0%, 43%, 71%, 71%, and 86% of the eyes in group 1. After IVIs of plasmin, VEGF, and vitreous tap had been performed one to three times, lens subluxation was noted in 11%, 22%, 44%, 44%, and 67% of the eyes in group 2. The eyes in group 3 and 4 did not show signs of lens subluxation after VEGF IVIs and vitreous tap. Histology confirmed zonular fiber damage in the eyes treated with plasmin.

Conclusions: The incidence of lens subluxation increased following plasmin injections in the eyes, and this was aggravated by the subsequent high VEGF level in the eyes and vitreous tapping. Zonular fibers were disrupted following plasmin treatment. These effects should be kept in mind when using plasmin enzymes in patients with vitreoretinal abnormalities.

Show MeSH
Related in: MedlinePlus