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Lens subluxation after plasmin and SF6 injections in rabbit eyes.

Wu WC, Liu CH, Wang NK, Chen KJ, Chen TL, Hwang YS, Liao PJ, Li LM, Lai CC - PLoS ONE (2014)

Bottom Line: Histology confirmed zonular fiber damage in the eyes treated with plasmin.Zonular fibers were disrupted following plasmin treatment.These effects should be kept in mind when using plasmin enzymes in patients with vitreoretinal abnormalities.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, Chang Gung Memorial Hospital, Taoyuan, Taiwan; Chang Gung University, College of Medicine, Taoyuan, Taiwan.

ABSTRACT

Purpose: To investigate the rate of lens subluxation following plasmin and/or SF6 injections in eyes, and whether a subsequent elevated level of vascular endothelial growth factor (VEGF) and vitreous tap would aggravate subluxation.

Methods: Four groups of rabbits were used. Group 1 received an intravitreal injection (IVI) of plasmin and SF6 in the right eye; group 2 received an IVI of plasmin in the right eye; group 3 received an IVI of SF6 in the right eye; and group 4 received an IVI of balanced salt solution in the right eye. After treatment, IVIs of VEGF were given and vitreous tap was performed three times, followed by clinical observation of lens subluxation and scanning electronic microscope evaluation of the zonular fibers.

Results: After IVIs of plasmin and SF6, and VEGF and vitreous tap had been performed one to three times, lens subluxation was noted in 0%, 43%, 71%, 71%, and 86% of the eyes in group 1. After IVIs of plasmin, VEGF, and vitreous tap had been performed one to three times, lens subluxation was noted in 11%, 22%, 44%, 44%, and 67% of the eyes in group 2. The eyes in group 3 and 4 did not show signs of lens subluxation after VEGF IVIs and vitreous tap. Histology confirmed zonular fiber damage in the eyes treated with plasmin.

Conclusions: The incidence of lens subluxation increased following plasmin injections in the eyes, and this was aggravated by the subsequent high VEGF level in the eyes and vitreous tapping. Zonular fibers were disrupted following plasmin treatment. These effects should be kept in mind when using plasmin enzymes in patients with vitreoretinal abnormalities.

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Related in: MedlinePlus

Lens subluxation following plasmin treatment.Representative figures of lens subluxation after intravitreal injections of plasmin and SF6, or plasmin alone, with a subsequent high intraocular level of vascular endothelial growth factor (VEGF), and vitreous tap. After intravitreal injections of plasmin with/without SF6, VEGF, and vitreous tap, lens subluxation was noted in the eyes treated with plasmin and SF6 (plasmin+SF6 group) or plasmin alone (plasmin group) (white arrows). Lens subluxation was progressive in some cases (case number 711, wr17, and wr13) and stationary in others (case 423, and 513). IVI, intravitreal injection.
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pone-0112957-g002: Lens subluxation following plasmin treatment.Representative figures of lens subluxation after intravitreal injections of plasmin and SF6, or plasmin alone, with a subsequent high intraocular level of vascular endothelial growth factor (VEGF), and vitreous tap. After intravitreal injections of plasmin with/without SF6, VEGF, and vitreous tap, lens subluxation was noted in the eyes treated with plasmin and SF6 (plasmin+SF6 group) or plasmin alone (plasmin group) (white arrows). Lens subluxation was progressive in some cases (case number 711, wr17, and wr13) and stationary in others (case 423, and 513). IVI, intravitreal injection.

Mentions: After treatment, the animals were checked every week to examine whether there was signs of lens subluxation until they were sacrificed (Figure 2). The status of lens subluxation in the eyes was verified by two independent observers.


Lens subluxation after plasmin and SF6 injections in rabbit eyes.

Wu WC, Liu CH, Wang NK, Chen KJ, Chen TL, Hwang YS, Liao PJ, Li LM, Lai CC - PLoS ONE (2014)

Lens subluxation following plasmin treatment.Representative figures of lens subluxation after intravitreal injections of plasmin and SF6, or plasmin alone, with a subsequent high intraocular level of vascular endothelial growth factor (VEGF), and vitreous tap. After intravitreal injections of plasmin with/without SF6, VEGF, and vitreous tap, lens subluxation was noted in the eyes treated with plasmin and SF6 (plasmin+SF6 group) or plasmin alone (plasmin group) (white arrows). Lens subluxation was progressive in some cases (case number 711, wr17, and wr13) and stationary in others (case 423, and 513). IVI, intravitreal injection.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4232556&req=5

pone-0112957-g002: Lens subluxation following plasmin treatment.Representative figures of lens subluxation after intravitreal injections of plasmin and SF6, or plasmin alone, with a subsequent high intraocular level of vascular endothelial growth factor (VEGF), and vitreous tap. After intravitreal injections of plasmin with/without SF6, VEGF, and vitreous tap, lens subluxation was noted in the eyes treated with plasmin and SF6 (plasmin+SF6 group) or plasmin alone (plasmin group) (white arrows). Lens subluxation was progressive in some cases (case number 711, wr17, and wr13) and stationary in others (case 423, and 513). IVI, intravitreal injection.
Mentions: After treatment, the animals were checked every week to examine whether there was signs of lens subluxation until they were sacrificed (Figure 2). The status of lens subluxation in the eyes was verified by two independent observers.

Bottom Line: Histology confirmed zonular fiber damage in the eyes treated with plasmin.Zonular fibers were disrupted following plasmin treatment.These effects should be kept in mind when using plasmin enzymes in patients with vitreoretinal abnormalities.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, Chang Gung Memorial Hospital, Taoyuan, Taiwan; Chang Gung University, College of Medicine, Taoyuan, Taiwan.

ABSTRACT

Purpose: To investigate the rate of lens subluxation following plasmin and/or SF6 injections in eyes, and whether a subsequent elevated level of vascular endothelial growth factor (VEGF) and vitreous tap would aggravate subluxation.

Methods: Four groups of rabbits were used. Group 1 received an intravitreal injection (IVI) of plasmin and SF6 in the right eye; group 2 received an IVI of plasmin in the right eye; group 3 received an IVI of SF6 in the right eye; and group 4 received an IVI of balanced salt solution in the right eye. After treatment, IVIs of VEGF were given and vitreous tap was performed three times, followed by clinical observation of lens subluxation and scanning electronic microscope evaluation of the zonular fibers.

Results: After IVIs of plasmin and SF6, and VEGF and vitreous tap had been performed one to three times, lens subluxation was noted in 0%, 43%, 71%, 71%, and 86% of the eyes in group 1. After IVIs of plasmin, VEGF, and vitreous tap had been performed one to three times, lens subluxation was noted in 11%, 22%, 44%, 44%, and 67% of the eyes in group 2. The eyes in group 3 and 4 did not show signs of lens subluxation after VEGF IVIs and vitreous tap. Histology confirmed zonular fiber damage in the eyes treated with plasmin.

Conclusions: The incidence of lens subluxation increased following plasmin injections in the eyes, and this was aggravated by the subsequent high VEGF level in the eyes and vitreous tapping. Zonular fibers were disrupted following plasmin treatment. These effects should be kept in mind when using plasmin enzymes in patients with vitreoretinal abnormalities.

Show MeSH
Related in: MedlinePlus