Limits...
Whole-genome expression analysis in the third instar larval midgut of Drosophila melanogaster.

Harrop TW, Pearce SL, Daborn PJ, Batterham P - G3 (Bethesda) (2014)

Bottom Line: The data support functional diversification in subsections of the midgut.Analysis of the expression of gene families that are implicated in the metabolism of xenobiotics suggests that metabolism may not be uniform along the midgut.These data provide a starting point for investigating gene expression and xenobiotic metabolism and other functions of the larval midgut.

View Article: PubMed Central - PubMed

Affiliation: Department of Genetics, Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Victoria, Australia twharrop@gmail.com p.batterham@unimelb.edu.au.

Show MeSH

Related in: MedlinePlus

Typical expression in midguts dissected from larvae producing GFP under the control of the Cyp4d2 promoter. GFP was expressed in M2, M4, M6, M9, M12, and M13, allowing the sections indicated to be harvested for sequencing. GFP, green fluorescent protein.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4232545&req=5

fig4: Typical expression in midguts dissected from larvae producing GFP under the control of the Cyp4d2 promoter. GFP was expressed in M2, M4, M6, M9, M12, and M13, allowing the sections indicated to be harvested for sequencing. GFP, green fluorescent protein.

Mentions: Expression of GFP in subsections of the midgut, driven with the promoter of the Cyp4d2 gene, was used to guide dissection of midguts for sequencing (Figure 4). As an initial validation step, the RNA sequencing results were compared with FlyAtlas expression data (Chintapalli et al. 2007). For each of eight tissues—the central nervous system, trachea, fat body, hindgut, salivary gland, Malpighian tubule, carcass, and midgut—two genes that are reported by FlyAtlas to be highly and specifically expressed in that tissue were chosen. The expression of these 16 genes was examined in the midgut transcriptome dataset. Only the two genes that are reported by FlyAtlas to be expressed in the midgut were enriched in the midgut transcriptome (Figure 5A). This finding suggests that the midguts were cleanly dissected without contamination with RNA from other tissues.


Whole-genome expression analysis in the third instar larval midgut of Drosophila melanogaster.

Harrop TW, Pearce SL, Daborn PJ, Batterham P - G3 (Bethesda) (2014)

Typical expression in midguts dissected from larvae producing GFP under the control of the Cyp4d2 promoter. GFP was expressed in M2, M4, M6, M9, M12, and M13, allowing the sections indicated to be harvested for sequencing. GFP, green fluorescent protein.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4232545&req=5

fig4: Typical expression in midguts dissected from larvae producing GFP under the control of the Cyp4d2 promoter. GFP was expressed in M2, M4, M6, M9, M12, and M13, allowing the sections indicated to be harvested for sequencing. GFP, green fluorescent protein.
Mentions: Expression of GFP in subsections of the midgut, driven with the promoter of the Cyp4d2 gene, was used to guide dissection of midguts for sequencing (Figure 4). As an initial validation step, the RNA sequencing results were compared with FlyAtlas expression data (Chintapalli et al. 2007). For each of eight tissues—the central nervous system, trachea, fat body, hindgut, salivary gland, Malpighian tubule, carcass, and midgut—two genes that are reported by FlyAtlas to be highly and specifically expressed in that tissue were chosen. The expression of these 16 genes was examined in the midgut transcriptome dataset. Only the two genes that are reported by FlyAtlas to be expressed in the midgut were enriched in the midgut transcriptome (Figure 5A). This finding suggests that the midguts were cleanly dissected without contamination with RNA from other tissues.

Bottom Line: The data support functional diversification in subsections of the midgut.Analysis of the expression of gene families that are implicated in the metabolism of xenobiotics suggests that metabolism may not be uniform along the midgut.These data provide a starting point for investigating gene expression and xenobiotic metabolism and other functions of the larval midgut.

View Article: PubMed Central - PubMed

Affiliation: Department of Genetics, Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Victoria, Australia twharrop@gmail.com p.batterham@unimelb.edu.au.

Show MeSH
Related in: MedlinePlus