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Novel X-linked genes revealed by quantitative polymerase chain reaction in the green anole, Anolis carolinensis.

Rovatsos M, Altmanová M, Pokorná MJ, Kratochvíl L - G3 (Bethesda) (2014)

Bottom Line: Assuming that all genes located on these scaffolds should be localized to the ACA X chromosome, we more than doubled the number of known X-linked genes in ACA, from 106 to 250.While demonstrating that the gene content of chromosome X in ACA and GGA15 is largely conserved, we nevertheless showed that numerous interchromosomal rearrangements had occurred since the splitting of the chicken and anole evolutionary lineages.The identification of novel genes linked to the X chromosome and absent on the Y chromosome in the model lizard species contributes to ongoing research as to the evolution of sex determination in reptiles and provides important information for future comparative and functional genomics.

View Article: PubMed Central - PubMed

Affiliation: Department of Ecology, Faculty of Science, Charles University in Prague, Prague, 128 44.

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Homology between nine A. carolinensis contigs and the Gallus gallus chromosome 15. The homology was predicted by the Genomicus database (http://www.genomicus.biologie.ens.fr/genomicus-75.02/cgi-bin/search.pl).
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fig3: Homology between nine A. carolinensis contigs and the Gallus gallus chromosome 15. The homology was predicted by the Genomicus database (http://www.genomicus.biologie.ens.fr/genomicus-75.02/cgi-bin/search.pl).

Mentions: Comparison of the topology between the X-linked ACA contigs and GGA 15 (Figure 3), as illustrated by the Genomicus database (http://www.genomicus.biologie.ens.fr/genomicus-75.02/cgi-bin/search.pl; Louis et al. 2013), shows that despite high conservation of the chromosome, several intrachromosomal rearrangements, such as inversions, probably occurred at this chromosome after the divergence of ACA and GGA from their common ancestor. Similarly, no interchromosomal but numerous intrachromosomal rearrangements have been documented in the microchromosomes of chicken, turkey, and zebra finch (Lithgow et al. 2014). Several pericentromeric inversions have been revealed in the chromosomal pairs 1–4 of ACA by in situ hybridization with BACs, but their functional importance remains unclear (Alföldi et al. 2011). We should keep in mind, however, a recent counterexample based on fluorescent in situ hybridization mapping of 11 markers whereby a high level of synteny was revealed between ACA chromosome 6 and its homologous chromosome in snakes (Z in colubroid snakes) without any detectable large-scale chromosomal rearrangements (Vicoso et al. 2013).


Novel X-linked genes revealed by quantitative polymerase chain reaction in the green anole, Anolis carolinensis.

Rovatsos M, Altmanová M, Pokorná MJ, Kratochvíl L - G3 (Bethesda) (2014)

Homology between nine A. carolinensis contigs and the Gallus gallus chromosome 15. The homology was predicted by the Genomicus database (http://www.genomicus.biologie.ens.fr/genomicus-75.02/cgi-bin/search.pl).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4232536&req=5

fig3: Homology between nine A. carolinensis contigs and the Gallus gallus chromosome 15. The homology was predicted by the Genomicus database (http://www.genomicus.biologie.ens.fr/genomicus-75.02/cgi-bin/search.pl).
Mentions: Comparison of the topology between the X-linked ACA contigs and GGA 15 (Figure 3), as illustrated by the Genomicus database (http://www.genomicus.biologie.ens.fr/genomicus-75.02/cgi-bin/search.pl; Louis et al. 2013), shows that despite high conservation of the chromosome, several intrachromosomal rearrangements, such as inversions, probably occurred at this chromosome after the divergence of ACA and GGA from their common ancestor. Similarly, no interchromosomal but numerous intrachromosomal rearrangements have been documented in the microchromosomes of chicken, turkey, and zebra finch (Lithgow et al. 2014). Several pericentromeric inversions have been revealed in the chromosomal pairs 1–4 of ACA by in situ hybridization with BACs, but their functional importance remains unclear (Alföldi et al. 2011). We should keep in mind, however, a recent counterexample based on fluorescent in situ hybridization mapping of 11 markers whereby a high level of synteny was revealed between ACA chromosome 6 and its homologous chromosome in snakes (Z in colubroid snakes) without any detectable large-scale chromosomal rearrangements (Vicoso et al. 2013).

Bottom Line: Assuming that all genes located on these scaffolds should be localized to the ACA X chromosome, we more than doubled the number of known X-linked genes in ACA, from 106 to 250.While demonstrating that the gene content of chromosome X in ACA and GGA15 is largely conserved, we nevertheless showed that numerous interchromosomal rearrangements had occurred since the splitting of the chicken and anole evolutionary lineages.The identification of novel genes linked to the X chromosome and absent on the Y chromosome in the model lizard species contributes to ongoing research as to the evolution of sex determination in reptiles and provides important information for future comparative and functional genomics.

View Article: PubMed Central - PubMed

Affiliation: Department of Ecology, Faculty of Science, Charles University in Prague, Prague, 128 44.

Show MeSH
Related in: MedlinePlus