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Novel X-linked genes revealed by quantitative polymerase chain reaction in the green anole, Anolis carolinensis.

Rovatsos M, Altmanová M, Pokorná MJ, Kratochvíl L - G3 (Bethesda) (2014)

Bottom Line: Assuming that all genes located on these scaffolds should be localized to the ACA X chromosome, we more than doubled the number of known X-linked genes in ACA, from 106 to 250.While demonstrating that the gene content of chromosome X in ACA and GGA15 is largely conserved, we nevertheless showed that numerous interchromosomal rearrangements had occurred since the splitting of the chicken and anole evolutionary lineages.The identification of novel genes linked to the X chromosome and absent on the Y chromosome in the model lizard species contributes to ongoing research as to the evolution of sex determination in reptiles and provides important information for future comparative and functional genomics.

View Article: PubMed Central - PubMed

Affiliation: Department of Ecology, Faculty of Science, Charles University in Prague, Prague, 128 44.

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Male-to-female relative gene dosage ratios for genes tested by quantitative polymerase chain reaction in A. carolinensis. Value 1.0 is expected for autosomal or pseudoautosomal genes, while value 0.5 is consistent with X-specific position.
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fig1: Male-to-female relative gene dosage ratios for genes tested by quantitative polymerase chain reaction in A. carolinensis. Value 1.0 is expected for autosomal or pseudoautosomal genes, while value 0.5 is consistent with X-specific position.

Mentions: The relative gene dosage ratios between males and females were estimated by qPCR for two genes from chromosome 5 (contig No. NC_014780), one gene from chromosome 6 (NC_014781), six genes from the known X-linked region of ACA (NW_003338829), and 32 putative X-linked genes assigned to seven unanchored scaffolds (see Figure 1, Table 1, Table 2, and Table S1). In all cases, the values from the gene EF1a were used for normalization.


Novel X-linked genes revealed by quantitative polymerase chain reaction in the green anole, Anolis carolinensis.

Rovatsos M, Altmanová M, Pokorná MJ, Kratochvíl L - G3 (Bethesda) (2014)

Male-to-female relative gene dosage ratios for genes tested by quantitative polymerase chain reaction in A. carolinensis. Value 1.0 is expected for autosomal or pseudoautosomal genes, while value 0.5 is consistent with X-specific position.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4232536&req=5

fig1: Male-to-female relative gene dosage ratios for genes tested by quantitative polymerase chain reaction in A. carolinensis. Value 1.0 is expected for autosomal or pseudoautosomal genes, while value 0.5 is consistent with X-specific position.
Mentions: The relative gene dosage ratios between males and females were estimated by qPCR for two genes from chromosome 5 (contig No. NC_014780), one gene from chromosome 6 (NC_014781), six genes from the known X-linked region of ACA (NW_003338829), and 32 putative X-linked genes assigned to seven unanchored scaffolds (see Figure 1, Table 1, Table 2, and Table S1). In all cases, the values from the gene EF1a were used for normalization.

Bottom Line: Assuming that all genes located on these scaffolds should be localized to the ACA X chromosome, we more than doubled the number of known X-linked genes in ACA, from 106 to 250.While demonstrating that the gene content of chromosome X in ACA and GGA15 is largely conserved, we nevertheless showed that numerous interchromosomal rearrangements had occurred since the splitting of the chicken and anole evolutionary lineages.The identification of novel genes linked to the X chromosome and absent on the Y chromosome in the model lizard species contributes to ongoing research as to the evolution of sex determination in reptiles and provides important information for future comparative and functional genomics.

View Article: PubMed Central - PubMed

Affiliation: Department of Ecology, Faculty of Science, Charles University in Prague, Prague, 128 44.

Show MeSH
Related in: MedlinePlus