Novel X-linked genes revealed by quantitative polymerase chain reaction in the green anole, Anolis carolinensis.
Bottom Line: Assuming that all genes located on these scaffolds should be localized to the ACA X chromosome, we more than doubled the number of known X-linked genes in ACA, from 106 to 250.While demonstrating that the gene content of chromosome X in ACA and GGA15 is largely conserved, we nevertheless showed that numerous interchromosomal rearrangements had occurred since the splitting of the chicken and anole evolutionary lineages.The identification of novel genes linked to the X chromosome and absent on the Y chromosome in the model lizard species contributes to ongoing research as to the evolution of sex determination in reptiles and provides important information for future comparative and functional genomics.
Affiliation: Department of Ecology, Faculty of Science, Charles University in Prague, Prague, 128 44.Show MeSH
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Mentions: The relative gene dosage ratios between males and females were estimated by qPCR for two genes from chromosome 5 (contig No. NC_014780), one gene from chromosome 6 (NC_014781), six genes from the known X-linked region of ACA (NW_003338829), and 32 putative X-linked genes assigned to seven unanchored scaffolds (see Figure 1, Table 1, Table 2, and Table S1). In all cases, the values from the gene EF1a were used for normalization.
Affiliation: Department of Ecology, Faculty of Science, Charles University in Prague, Prague, 128 44.