Limits...
Chronic maternal vitamin B12 restriction induced changes in body composition & glucose metabolism in the Wistar rat offspring are partly correctable by rehabilitation.

Kumar KA, Lalitha A, Reddy U, Chandak GR, Sengupta S, Raghunath M - PLoS ONE (2014)

Bottom Line: Dietary vitamin B12 restriction increased body weight but decreased LBM% and FFM% but not the percent of tissue associated fat (TAF%) in dams.Maternal B12R decreased LBM% and FFM% in the male offspring, but their TAF%, basal and insulin stimulated glucose uptake by diaphragm were unaltered.B12R offspring had increased oxidative stress and decreased antioxidant status.

View Article: PubMed Central - PubMed

Affiliation: Division of Endocrinology and Metabolism, National Institute of Nutrition, Indian Council of Medical Research (ICMR), Hyderabad, Telangana, India.

ABSTRACT
Maternal under-nutrition increases the risk of developing metabolic diseases. We studied the effects of chronic maternal dietary vitamin B12 restriction on lean body mass (LBM), fat free mass (FFM), muscle function, glucose tolerance and metabolism in Wistar rat offspring. Prevention/reversibility of changes by rehabilitating restricted mothers from conception or parturition and their offspring from weaning was assessed. Female weaning Wistar rats (n = 30) were fed ad libitum for 12 weeks, a control diet (n = 6) or the same with 40% restriction of vitamin B12 (B12R) (n = 24); after confirming deficiency, were mated with control males. Six each of pregnant B12R dams were rehabilitated from conception and parturition and their offspring weaned to control diet. While offspring of six B12R dams were weaned to control diet, those of the remaining six B12R dams continued on B12R diet. Biochemical parameters and body composition were determined in dams before mating and in male offspring at 3, 6, 9 and 12 months of their age. Dietary vitamin B12 restriction increased body weight but decreased LBM% and FFM% but not the percent of tissue associated fat (TAF%) in dams. Maternal B12R decreased LBM% and FFM% in the male offspring, but their TAF%, basal and insulin stimulated glucose uptake by diaphragm were unaltered. At 12 months age, B12R offspring had higher (than controls) fasting plasma glucose, insulin, HOMA-IR and impaired glucose tolerance. Their hepatic gluconeogenic enzyme activities were increased. B12R offspring had increased oxidative stress and decreased antioxidant status. Changes in body composition, glucose metabolism and stress were reversed by rehabilitating B12R dams from conception, whereas rehabilitation from parturition and weaning corrected them partially, highlighting the importance of vitamin B12 during pregnancy and lactation on growth, muscle development, glucose tolerance and metabolism in the offspring.

Show MeSH

Related in: MedlinePlus

Glucose and insulin AUC during oral glucose tolerance test.Area Under Curves (AUC) of Glucose and insulin at different time points in Wistar rat male offspring fed different diets: Control (C), B12 restricted (B12R), B12 rehabilitation from conception (B12RC), B12 rehabilitation from parturition (B12RP) and B12 rehabilitation from weaning (B12RW). (a) Glucose AUC (moles/L/hr), (b) Insulin AUC (pmoles/L/hr). Values are mean ± SE (n = 6). Bars with different superscripts (a/b/c) are significantly different from one another at p<0.05 by one way ANOVA/LSD test.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4232526&req=5

pone-0112991-g004: Glucose and insulin AUC during oral glucose tolerance test.Area Under Curves (AUC) of Glucose and insulin at different time points in Wistar rat male offspring fed different diets: Control (C), B12 restricted (B12R), B12 rehabilitation from conception (B12RC), B12 rehabilitation from parturition (B12RP) and B12 rehabilitation from weaning (B12RW). (a) Glucose AUC (moles/L/hr), (b) Insulin AUC (pmoles/L/hr). Values are mean ± SE (n = 6). Bars with different superscripts (a/b/c) are significantly different from one another at p<0.05 by one way ANOVA/LSD test.

Mentions: In line with fasting hyperglycemia and hyperinsulinemia B12R offspring had higher (than controls) AUC for glucose and insulin during the oral glucose tolerance test, at 12 months of age but not earlier. While none of the rehabilitation regimes corrected the increase in AUC glucose during the OGTT (Figure 4), the increased insulin AUC was reversed by B12RC, but the mitigation was only partial by B12RP and B12RW (Figure 4). The ratio of AUC glucose and AUC insulin was comparable among groups at all-time points tested (Table S4).


Chronic maternal vitamin B12 restriction induced changes in body composition & glucose metabolism in the Wistar rat offspring are partly correctable by rehabilitation.

Kumar KA, Lalitha A, Reddy U, Chandak GR, Sengupta S, Raghunath M - PLoS ONE (2014)

Glucose and insulin AUC during oral glucose tolerance test.Area Under Curves (AUC) of Glucose and insulin at different time points in Wistar rat male offspring fed different diets: Control (C), B12 restricted (B12R), B12 rehabilitation from conception (B12RC), B12 rehabilitation from parturition (B12RP) and B12 rehabilitation from weaning (B12RW). (a) Glucose AUC (moles/L/hr), (b) Insulin AUC (pmoles/L/hr). Values are mean ± SE (n = 6). Bars with different superscripts (a/b/c) are significantly different from one another at p<0.05 by one way ANOVA/LSD test.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4232526&req=5

pone-0112991-g004: Glucose and insulin AUC during oral glucose tolerance test.Area Under Curves (AUC) of Glucose and insulin at different time points in Wistar rat male offspring fed different diets: Control (C), B12 restricted (B12R), B12 rehabilitation from conception (B12RC), B12 rehabilitation from parturition (B12RP) and B12 rehabilitation from weaning (B12RW). (a) Glucose AUC (moles/L/hr), (b) Insulin AUC (pmoles/L/hr). Values are mean ± SE (n = 6). Bars with different superscripts (a/b/c) are significantly different from one another at p<0.05 by one way ANOVA/LSD test.
Mentions: In line with fasting hyperglycemia and hyperinsulinemia B12R offspring had higher (than controls) AUC for glucose and insulin during the oral glucose tolerance test, at 12 months of age but not earlier. While none of the rehabilitation regimes corrected the increase in AUC glucose during the OGTT (Figure 4), the increased insulin AUC was reversed by B12RC, but the mitigation was only partial by B12RP and B12RW (Figure 4). The ratio of AUC glucose and AUC insulin was comparable among groups at all-time points tested (Table S4).

Bottom Line: Dietary vitamin B12 restriction increased body weight but decreased LBM% and FFM% but not the percent of tissue associated fat (TAF%) in dams.Maternal B12R decreased LBM% and FFM% in the male offspring, but their TAF%, basal and insulin stimulated glucose uptake by diaphragm were unaltered.B12R offspring had increased oxidative stress and decreased antioxidant status.

View Article: PubMed Central - PubMed

Affiliation: Division of Endocrinology and Metabolism, National Institute of Nutrition, Indian Council of Medical Research (ICMR), Hyderabad, Telangana, India.

ABSTRACT
Maternal under-nutrition increases the risk of developing metabolic diseases. We studied the effects of chronic maternal dietary vitamin B12 restriction on lean body mass (LBM), fat free mass (FFM), muscle function, glucose tolerance and metabolism in Wistar rat offspring. Prevention/reversibility of changes by rehabilitating restricted mothers from conception or parturition and their offspring from weaning was assessed. Female weaning Wistar rats (n = 30) were fed ad libitum for 12 weeks, a control diet (n = 6) or the same with 40% restriction of vitamin B12 (B12R) (n = 24); after confirming deficiency, were mated with control males. Six each of pregnant B12R dams were rehabilitated from conception and parturition and their offspring weaned to control diet. While offspring of six B12R dams were weaned to control diet, those of the remaining six B12R dams continued on B12R diet. Biochemical parameters and body composition were determined in dams before mating and in male offspring at 3, 6, 9 and 12 months of their age. Dietary vitamin B12 restriction increased body weight but decreased LBM% and FFM% but not the percent of tissue associated fat (TAF%) in dams. Maternal B12R decreased LBM% and FFM% in the male offspring, but their TAF%, basal and insulin stimulated glucose uptake by diaphragm were unaltered. At 12 months age, B12R offspring had higher (than controls) fasting plasma glucose, insulin, HOMA-IR and impaired glucose tolerance. Their hepatic gluconeogenic enzyme activities were increased. B12R offspring had increased oxidative stress and decreased antioxidant status. Changes in body composition, glucose metabolism and stress were reversed by rehabilitating B12R dams from conception, whereas rehabilitation from parturition and weaning corrected them partially, highlighting the importance of vitamin B12 during pregnancy and lactation on growth, muscle development, glucose tolerance and metabolism in the offspring.

Show MeSH
Related in: MedlinePlus