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Chronic maternal vitamin B12 restriction induced changes in body composition & glucose metabolism in the Wistar rat offspring are partly correctable by rehabilitation.

Kumar KA, Lalitha A, Reddy U, Chandak GR, Sengupta S, Raghunath M - PLoS ONE (2014)

Bottom Line: Dietary vitamin B12 restriction increased body weight but decreased LBM% and FFM% but not the percent of tissue associated fat (TAF%) in dams.Maternal B12R decreased LBM% and FFM% in the male offspring, but their TAF%, basal and insulin stimulated glucose uptake by diaphragm were unaltered.B12R offspring had increased oxidative stress and decreased antioxidant status.

View Article: PubMed Central - PubMed

Affiliation: Division of Endocrinology and Metabolism, National Institute of Nutrition, Indian Council of Medical Research (ICMR), Hyderabad, Telangana, India.

ABSTRACT
Maternal under-nutrition increases the risk of developing metabolic diseases. We studied the effects of chronic maternal dietary vitamin B12 restriction on lean body mass (LBM), fat free mass (FFM), muscle function, glucose tolerance and metabolism in Wistar rat offspring. Prevention/reversibility of changes by rehabilitating restricted mothers from conception or parturition and their offspring from weaning was assessed. Female weaning Wistar rats (n = 30) were fed ad libitum for 12 weeks, a control diet (n = 6) or the same with 40% restriction of vitamin B12 (B12R) (n = 24); after confirming deficiency, were mated with control males. Six each of pregnant B12R dams were rehabilitated from conception and parturition and their offspring weaned to control diet. While offspring of six B12R dams were weaned to control diet, those of the remaining six B12R dams continued on B12R diet. Biochemical parameters and body composition were determined in dams before mating and in male offspring at 3, 6, 9 and 12 months of their age. Dietary vitamin B12 restriction increased body weight but decreased LBM% and FFM% but not the percent of tissue associated fat (TAF%) in dams. Maternal B12R decreased LBM% and FFM% in the male offspring, but their TAF%, basal and insulin stimulated glucose uptake by diaphragm were unaltered. At 12 months age, B12R offspring had higher (than controls) fasting plasma glucose, insulin, HOMA-IR and impaired glucose tolerance. Their hepatic gluconeogenic enzyme activities were increased. B12R offspring had increased oxidative stress and decreased antioxidant status. Changes in body composition, glucose metabolism and stress were reversed by rehabilitating B12R dams from conception, whereas rehabilitation from parturition and weaning corrected them partially, highlighting the importance of vitamin B12 during pregnancy and lactation on growth, muscle development, glucose tolerance and metabolism in the offspring.

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Body composition changes in male offspring.Body composition changes at different time points in Wistar rat male offspring fed different diets: Control (C), B12 restricted (B12R), B12 rehabilitation from conception (B12RC), B12 rehabilitation from parturition (B12RP) and B12 rehabilitation from weaning (B12RW). (a) Lean Body Mass%, (b) Fat Free Mass% (c) Tissue Associated fat%. Values are mean ± SE (n = 6). Bars with different superscripts (a/b/c) are significantly different from one another at p<0.05 by one way ANOVA/LSD test.
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pone-0112991-g002: Body composition changes in male offspring.Body composition changes at different time points in Wistar rat male offspring fed different diets: Control (C), B12 restricted (B12R), B12 rehabilitation from conception (B12RC), B12 rehabilitation from parturition (B12RP) and B12 rehabilitation from weaning (B12RW). (a) Lean Body Mass%, (b) Fat Free Mass% (c) Tissue Associated fat%. Values are mean ± SE (n = 6). Bars with different superscripts (a/b/c) are significantly different from one another at p<0.05 by one way ANOVA/LSD test.

Mentions: Both at three and twelve months of their age, LBM% and FFM% of B12R offspring were lower than those of controls (Figure 2). Although all three rehabilitation regimes appeared to correct these changes at three months of age, only B12RC and B12RP but not B12RW appeared to do so at twelve months of age. These findings suggest the importance of dams’ vitamin B12 status during pregnancy and lactation in programming/modulating the body composition of the offspring.


Chronic maternal vitamin B12 restriction induced changes in body composition & glucose metabolism in the Wistar rat offspring are partly correctable by rehabilitation.

Kumar KA, Lalitha A, Reddy U, Chandak GR, Sengupta S, Raghunath M - PLoS ONE (2014)

Body composition changes in male offspring.Body composition changes at different time points in Wistar rat male offspring fed different diets: Control (C), B12 restricted (B12R), B12 rehabilitation from conception (B12RC), B12 rehabilitation from parturition (B12RP) and B12 rehabilitation from weaning (B12RW). (a) Lean Body Mass%, (b) Fat Free Mass% (c) Tissue Associated fat%. Values are mean ± SE (n = 6). Bars with different superscripts (a/b/c) are significantly different from one another at p<0.05 by one way ANOVA/LSD test.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4232526&req=5

pone-0112991-g002: Body composition changes in male offspring.Body composition changes at different time points in Wistar rat male offspring fed different diets: Control (C), B12 restricted (B12R), B12 rehabilitation from conception (B12RC), B12 rehabilitation from parturition (B12RP) and B12 rehabilitation from weaning (B12RW). (a) Lean Body Mass%, (b) Fat Free Mass% (c) Tissue Associated fat%. Values are mean ± SE (n = 6). Bars with different superscripts (a/b/c) are significantly different from one another at p<0.05 by one way ANOVA/LSD test.
Mentions: Both at three and twelve months of their age, LBM% and FFM% of B12R offspring were lower than those of controls (Figure 2). Although all three rehabilitation regimes appeared to correct these changes at three months of age, only B12RC and B12RP but not B12RW appeared to do so at twelve months of age. These findings suggest the importance of dams’ vitamin B12 status during pregnancy and lactation in programming/modulating the body composition of the offspring.

Bottom Line: Dietary vitamin B12 restriction increased body weight but decreased LBM% and FFM% but not the percent of tissue associated fat (TAF%) in dams.Maternal B12R decreased LBM% and FFM% in the male offspring, but their TAF%, basal and insulin stimulated glucose uptake by diaphragm were unaltered.B12R offspring had increased oxidative stress and decreased antioxidant status.

View Article: PubMed Central - PubMed

Affiliation: Division of Endocrinology and Metabolism, National Institute of Nutrition, Indian Council of Medical Research (ICMR), Hyderabad, Telangana, India.

ABSTRACT
Maternal under-nutrition increases the risk of developing metabolic diseases. We studied the effects of chronic maternal dietary vitamin B12 restriction on lean body mass (LBM), fat free mass (FFM), muscle function, glucose tolerance and metabolism in Wistar rat offspring. Prevention/reversibility of changes by rehabilitating restricted mothers from conception or parturition and their offspring from weaning was assessed. Female weaning Wistar rats (n = 30) were fed ad libitum for 12 weeks, a control diet (n = 6) or the same with 40% restriction of vitamin B12 (B12R) (n = 24); after confirming deficiency, were mated with control males. Six each of pregnant B12R dams were rehabilitated from conception and parturition and their offspring weaned to control diet. While offspring of six B12R dams were weaned to control diet, those of the remaining six B12R dams continued on B12R diet. Biochemical parameters and body composition were determined in dams before mating and in male offspring at 3, 6, 9 and 12 months of their age. Dietary vitamin B12 restriction increased body weight but decreased LBM% and FFM% but not the percent of tissue associated fat (TAF%) in dams. Maternal B12R decreased LBM% and FFM% in the male offspring, but their TAF%, basal and insulin stimulated glucose uptake by diaphragm were unaltered. At 12 months age, B12R offspring had higher (than controls) fasting plasma glucose, insulin, HOMA-IR and impaired glucose tolerance. Their hepatic gluconeogenic enzyme activities were increased. B12R offspring had increased oxidative stress and decreased antioxidant status. Changes in body composition, glucose metabolism and stress were reversed by rehabilitating B12R dams from conception, whereas rehabilitation from parturition and weaning corrected them partially, highlighting the importance of vitamin B12 during pregnancy and lactation on growth, muscle development, glucose tolerance and metabolism in the offspring.

Show MeSH
Related in: MedlinePlus