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The Arabidopsis PLAT domain protein1 is critically involved in abiotic stress tolerance.

Hyun TK, van der Graaff E, Albacete A, Eom SH, Großkinsky DK, Böhm H, Janschek U, Rim Y, Ali WW, Kim SY, Roitsch T - PLoS ONE (2014)

Bottom Line: PLAT1 overexpression conferred increased abiotic stress tolerance, including cold, drought and salt stress, while loss-of-function resulted in opposite effects on abiotic stress tolerance.This suggests that PLAT1 represents a novel downstream target of the abscisic acid signalling pathway.The functional data obtained for PLAT1 support that PLAT-plant-stress proteins in general could be promising targets for improving abiotic stress tolerance without yield penalty.

View Article: PubMed Central - PubMed

Affiliation: Institute of Plant Sciences, University of Graz, Graz, Austria; Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju, Korea.

ABSTRACT
Despite the completion of the Arabidopsis genome sequence, for only a relatively low percentage of the encoded proteins experimental evidence concerning their function is available. Plant proteins that harbour a single PLAT (Polycystin, Lipoxygenase, Alpha-toxin and Triacylglycerol lipase) domain and belong to the PLAT-plant-stress protein family are ubiquitously present in monocot and dicots. However, the function of PLAT-plant-stress proteins is still poorly understood. Therefore, we have assessed the function of the uncharacterised Arabidopsis PLAT-plant-stress family members through a combination of functional genetic and physiological approaches. PLAT1 overexpression conferred increased abiotic stress tolerance, including cold, drought and salt stress, while loss-of-function resulted in opposite effects on abiotic stress tolerance. Strikingly, PLAT1 promoted growth under non-stressed conditions. Abiotic stress treatments induced PLAT1 expression and caused expansion of its expression domain. The ABF/ABRE transcription factors, which are positive mediators of abscisic acid signalling, activate PLAT1 promoter activity in transactivation assays and directly bind to the ABRE elements located in this promoter in electrophoretic mobility shift assays. This suggests that PLAT1 represents a novel downstream target of the abscisic acid signalling pathway. Thus, we showed that PLAT1 critically functions as positive regulator of abiotic stress tolerance, but also is involved in regulating plant growth, and thereby assigned a function to this previously uncharacterised PLAT domain protein. The functional data obtained for PLAT1 support that PLAT-plant-stress proteins in general could be promising targets for improving abiotic stress tolerance without yield penalty.

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Related in: MedlinePlus

PLAT1 loss-of-function reduces abiotic stress tolerance.(A) Salt stress tolerance in wild-type (Col-0) and plat1-1 seedlings irrigated with 200 mM NaCl for 14 d. n≥10 (B) Drought stress tolerance in wild-type and plat1-1 seedlings, following 14 d without watering. n≥10 (C) Cold stress tolerance in 7-d-old wild-type, plat1-1 and plat1-2 seedlings following 14 d of incubation at 8°C. n = 14. Scale bar = 1 cm.
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pone-0112946-g002: PLAT1 loss-of-function reduces abiotic stress tolerance.(A) Salt stress tolerance in wild-type (Col-0) and plat1-1 seedlings irrigated with 200 mM NaCl for 14 d. n≥10 (B) Drought stress tolerance in wild-type and plat1-1 seedlings, following 14 d without watering. n≥10 (C) Cold stress tolerance in 7-d-old wild-type, plat1-1 and plat1-2 seedlings following 14 d of incubation at 8°C. n = 14. Scale bar = 1 cm.

Mentions: The plat1-1 mutant was more sensitive to salt and drought stress, as well as cold stress conditions (Figure 2), evident by a reduction in root length upon growth at 8°C from 9.41±1.25 mm for Col-0 to 7.02±1.27 mm or 6.20±0.94 mm for plat1-1 and plat1-2, respectively (p<0.001, n = 14). In contrast, the plat2 mutant did not show obvious changes in salt stress tolerance (Figure 3). Together with the differential effect of salt stress on expression of the PLAT family members (Figure 1), these data support that only PLAT1 is involved in abiotic stress tolerance.


The Arabidopsis PLAT domain protein1 is critically involved in abiotic stress tolerance.

Hyun TK, van der Graaff E, Albacete A, Eom SH, Großkinsky DK, Böhm H, Janschek U, Rim Y, Ali WW, Kim SY, Roitsch T - PLoS ONE (2014)

PLAT1 loss-of-function reduces abiotic stress tolerance.(A) Salt stress tolerance in wild-type (Col-0) and plat1-1 seedlings irrigated with 200 mM NaCl for 14 d. n≥10 (B) Drought stress tolerance in wild-type and plat1-1 seedlings, following 14 d without watering. n≥10 (C) Cold stress tolerance in 7-d-old wild-type, plat1-1 and plat1-2 seedlings following 14 d of incubation at 8°C. n = 14. Scale bar = 1 cm.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4232524&req=5

pone-0112946-g002: PLAT1 loss-of-function reduces abiotic stress tolerance.(A) Salt stress tolerance in wild-type (Col-0) and plat1-1 seedlings irrigated with 200 mM NaCl for 14 d. n≥10 (B) Drought stress tolerance in wild-type and plat1-1 seedlings, following 14 d without watering. n≥10 (C) Cold stress tolerance in 7-d-old wild-type, plat1-1 and plat1-2 seedlings following 14 d of incubation at 8°C. n = 14. Scale bar = 1 cm.
Mentions: The plat1-1 mutant was more sensitive to salt and drought stress, as well as cold stress conditions (Figure 2), evident by a reduction in root length upon growth at 8°C from 9.41±1.25 mm for Col-0 to 7.02±1.27 mm or 6.20±0.94 mm for plat1-1 and plat1-2, respectively (p<0.001, n = 14). In contrast, the plat2 mutant did not show obvious changes in salt stress tolerance (Figure 3). Together with the differential effect of salt stress on expression of the PLAT family members (Figure 1), these data support that only PLAT1 is involved in abiotic stress tolerance.

Bottom Line: PLAT1 overexpression conferred increased abiotic stress tolerance, including cold, drought and salt stress, while loss-of-function resulted in opposite effects on abiotic stress tolerance.This suggests that PLAT1 represents a novel downstream target of the abscisic acid signalling pathway.The functional data obtained for PLAT1 support that PLAT-plant-stress proteins in general could be promising targets for improving abiotic stress tolerance without yield penalty.

View Article: PubMed Central - PubMed

Affiliation: Institute of Plant Sciences, University of Graz, Graz, Austria; Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju, Korea.

ABSTRACT
Despite the completion of the Arabidopsis genome sequence, for only a relatively low percentage of the encoded proteins experimental evidence concerning their function is available. Plant proteins that harbour a single PLAT (Polycystin, Lipoxygenase, Alpha-toxin and Triacylglycerol lipase) domain and belong to the PLAT-plant-stress protein family are ubiquitously present in monocot and dicots. However, the function of PLAT-plant-stress proteins is still poorly understood. Therefore, we have assessed the function of the uncharacterised Arabidopsis PLAT-plant-stress family members through a combination of functional genetic and physiological approaches. PLAT1 overexpression conferred increased abiotic stress tolerance, including cold, drought and salt stress, while loss-of-function resulted in opposite effects on abiotic stress tolerance. Strikingly, PLAT1 promoted growth under non-stressed conditions. Abiotic stress treatments induced PLAT1 expression and caused expansion of its expression domain. The ABF/ABRE transcription factors, which are positive mediators of abscisic acid signalling, activate PLAT1 promoter activity in transactivation assays and directly bind to the ABRE elements located in this promoter in electrophoretic mobility shift assays. This suggests that PLAT1 represents a novel downstream target of the abscisic acid signalling pathway. Thus, we showed that PLAT1 critically functions as positive regulator of abiotic stress tolerance, but also is involved in regulating plant growth, and thereby assigned a function to this previously uncharacterised PLAT domain protein. The functional data obtained for PLAT1 support that PLAT-plant-stress proteins in general could be promising targets for improving abiotic stress tolerance without yield penalty.

Show MeSH
Related in: MedlinePlus