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Lactobacillus casei-01 facilitates the ameliorative effects of proanthocyanidins extracted from lotus seedpod on learning and memory impairment in scopolamine-induced amnesia mice.

Xiao J, Li S, Sui Y, Wu Q, Li X, Xie B, Zhang M, Sun Z - PLoS ONE (2014)

Bottom Line: However, there has been no literature about whether Lactobacillus casei-01 (LC) enhances the ameliorative effects of LSPC on learning and memory abilities.As a result, the error times of H-LSPC+LC group were reduced by 41.59% and 68.75% relative to those of H-LSPC and LC groups respectively.Moreover, LC facilitated the ameliorative effects of H-LSPC on GSH-Px activity of colon, TAOC level, GSH-Px activity and ratio of T-SOD to MDA of brain and serum, and the inhibitory effects of H-LSPC on serum MDA level, brain nNOS mRNA level and AchE activity.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Functional Foods, Ministry of Agriculture/ Guangdong Key Laboratory of Agricultural Products Processing, Sericultural & Agri-Food Research Institute Guangdong Academy of Agricultural Sciences, 133 Yiheng Road, Dongguan Zhuang, Tianhe District, Guangzhou 510610, Guangdong, China.

ABSTRACT
Learning and memory abilities are associated with alterations in gut function. The two-way proanthocyanidins-microbiota interaction in vivo enhances the physiological activities of proanthocyanidins and promotes the regulation of gut function. Proanthocyanidins extracted from lotus seedpod (LSPC) have shown the memory-enhancing ability. However, there has been no literature about whether Lactobacillus casei-01 (LC) enhances the ameliorative effects of LSPC on learning and memory abilities. In this study, learning and memory abilities of scopolamine-induced amnesia mice were evaluated by Y-maze test after 20-day administration of LC (10(9) cfu/kg body weight (BW)), LSPC (low dose was 60 mg/kg BW (L-LSPC) and high dose was 90 mg/kg BW (H-LSPC)), or LSPC and LC combinations (L-LSPC+LC and H-LSPC+LC). Alterations in antioxidant defense ability and oxidative damage of brain, serum and colon, and brain cholinergic system were investigated as the possible mechanisms. As a result, the error times of H-LSPC+LC group were reduced by 41.59% and 68.75% relative to those of H-LSPC and LC groups respectively. LSPC and LC combinations ameliorated scopolamine-induced memory impairment by improving total antioxidant capacity (TAOC) level, glutathione peroxidase (GSH-Px) and total superoxide dismutase (T-SOD) activities of brain, serum and colon, suppressing malondialdehyde (MDA) level of brain, serum and colon, and inhibiting brain acetylcholinesterase (AchE), myeloperoxidase, total nitric oxide synthase and neural nitric oxide synthase (nNOS) activities, and nNOS mRNA level. Moreover, LC facilitated the ameliorative effects of H-LSPC on GSH-Px activity of colon, TAOC level, GSH-Px activity and ratio of T-SOD to MDA of brain and serum, and the inhibitory effects of H-LSPC on serum MDA level, brain nNOS mRNA level and AchE activity. These results indicated that LC promoted the memory-enhancing effect of LSPC in scopolamine-induced amnesia mice.

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Effects of combined proanthocyanidins extracted from lotus seedpod (LSPC) and Lactobacillus casei-01 (LC) on the mRNA level of neural nitric oxide synthase (nNOS).Groups without any same letters above the bars signify statistically significant differences (P<0.05). CON, SCOP, Piracetam and LC is control, vehicle scopolamine control, positive drug control and Lactobacillus casei-01 group, respectively. L-LSPC and H-LSPC is low and high dose of LSPC group, respectively. L-LSPC+LC is L-LSPC and LC combination group. H-LSPC+LC is H-LSPC and LC combination group.
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pone-0112773-g002: Effects of combined proanthocyanidins extracted from lotus seedpod (LSPC) and Lactobacillus casei-01 (LC) on the mRNA level of neural nitric oxide synthase (nNOS).Groups without any same letters above the bars signify statistically significant differences (P<0.05). CON, SCOP, Piracetam and LC is control, vehicle scopolamine control, positive drug control and Lactobacillus casei-01 group, respectively. L-LSPC and H-LSPC is low and high dose of LSPC group, respectively. L-LSPC+LC is L-LSPC and LC combination group. H-LSPC+LC is H-LSPC and LC combination group.

Mentions: The variations in brain mRNA level of nNOS were parallel to the alterations in brain nNOS activity in all the experimental groups. As shown in Fig. 2, SCOP group showed significantly higher nNOS mRNA level than CON group. LC group revealed a significant decrease in nNOS mRNA level relative to SCOP group (P<0.05), but showed significantly higher nNOS mRNA level than CON group (P<0.05). However, nNOS mRNA level in Piracetam, L-LSPC, H-LSPC, L-LSPC+LC and H-LSPC+LC groups was remarkably lower than that in both SCOP and LC groups (all P<0.05), and comparative with that in CON group. Furthermore, H-LSPC+LC group exhibited a significant decline in nNOS mRNA level relative to Piracetam, LC, L-LSPC, H-LSPC and L-LSPC+LC groups (all P<0.05).


Lactobacillus casei-01 facilitates the ameliorative effects of proanthocyanidins extracted from lotus seedpod on learning and memory impairment in scopolamine-induced amnesia mice.

Xiao J, Li S, Sui Y, Wu Q, Li X, Xie B, Zhang M, Sun Z - PLoS ONE (2014)

Effects of combined proanthocyanidins extracted from lotus seedpod (LSPC) and Lactobacillus casei-01 (LC) on the mRNA level of neural nitric oxide synthase (nNOS).Groups without any same letters above the bars signify statistically significant differences (P<0.05). CON, SCOP, Piracetam and LC is control, vehicle scopolamine control, positive drug control and Lactobacillus casei-01 group, respectively. L-LSPC and H-LSPC is low and high dose of LSPC group, respectively. L-LSPC+LC is L-LSPC and LC combination group. H-LSPC+LC is H-LSPC and LC combination group.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4232518&req=5

pone-0112773-g002: Effects of combined proanthocyanidins extracted from lotus seedpod (LSPC) and Lactobacillus casei-01 (LC) on the mRNA level of neural nitric oxide synthase (nNOS).Groups without any same letters above the bars signify statistically significant differences (P<0.05). CON, SCOP, Piracetam and LC is control, vehicle scopolamine control, positive drug control and Lactobacillus casei-01 group, respectively. L-LSPC and H-LSPC is low and high dose of LSPC group, respectively. L-LSPC+LC is L-LSPC and LC combination group. H-LSPC+LC is H-LSPC and LC combination group.
Mentions: The variations in brain mRNA level of nNOS were parallel to the alterations in brain nNOS activity in all the experimental groups. As shown in Fig. 2, SCOP group showed significantly higher nNOS mRNA level than CON group. LC group revealed a significant decrease in nNOS mRNA level relative to SCOP group (P<0.05), but showed significantly higher nNOS mRNA level than CON group (P<0.05). However, nNOS mRNA level in Piracetam, L-LSPC, H-LSPC, L-LSPC+LC and H-LSPC+LC groups was remarkably lower than that in both SCOP and LC groups (all P<0.05), and comparative with that in CON group. Furthermore, H-LSPC+LC group exhibited a significant decline in nNOS mRNA level relative to Piracetam, LC, L-LSPC, H-LSPC and L-LSPC+LC groups (all P<0.05).

Bottom Line: However, there has been no literature about whether Lactobacillus casei-01 (LC) enhances the ameliorative effects of LSPC on learning and memory abilities.As a result, the error times of H-LSPC+LC group were reduced by 41.59% and 68.75% relative to those of H-LSPC and LC groups respectively.Moreover, LC facilitated the ameliorative effects of H-LSPC on GSH-Px activity of colon, TAOC level, GSH-Px activity and ratio of T-SOD to MDA of brain and serum, and the inhibitory effects of H-LSPC on serum MDA level, brain nNOS mRNA level and AchE activity.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Functional Foods, Ministry of Agriculture/ Guangdong Key Laboratory of Agricultural Products Processing, Sericultural & Agri-Food Research Institute Guangdong Academy of Agricultural Sciences, 133 Yiheng Road, Dongguan Zhuang, Tianhe District, Guangzhou 510610, Guangdong, China.

ABSTRACT
Learning and memory abilities are associated with alterations in gut function. The two-way proanthocyanidins-microbiota interaction in vivo enhances the physiological activities of proanthocyanidins and promotes the regulation of gut function. Proanthocyanidins extracted from lotus seedpod (LSPC) have shown the memory-enhancing ability. However, there has been no literature about whether Lactobacillus casei-01 (LC) enhances the ameliorative effects of LSPC on learning and memory abilities. In this study, learning and memory abilities of scopolamine-induced amnesia mice were evaluated by Y-maze test after 20-day administration of LC (10(9) cfu/kg body weight (BW)), LSPC (low dose was 60 mg/kg BW (L-LSPC) and high dose was 90 mg/kg BW (H-LSPC)), or LSPC and LC combinations (L-LSPC+LC and H-LSPC+LC). Alterations in antioxidant defense ability and oxidative damage of brain, serum and colon, and brain cholinergic system were investigated as the possible mechanisms. As a result, the error times of H-LSPC+LC group were reduced by 41.59% and 68.75% relative to those of H-LSPC and LC groups respectively. LSPC and LC combinations ameliorated scopolamine-induced memory impairment by improving total antioxidant capacity (TAOC) level, glutathione peroxidase (GSH-Px) and total superoxide dismutase (T-SOD) activities of brain, serum and colon, suppressing malondialdehyde (MDA) level of brain, serum and colon, and inhibiting brain acetylcholinesterase (AchE), myeloperoxidase, total nitric oxide synthase and neural nitric oxide synthase (nNOS) activities, and nNOS mRNA level. Moreover, LC facilitated the ameliorative effects of H-LSPC on GSH-Px activity of colon, TAOC level, GSH-Px activity and ratio of T-SOD to MDA of brain and serum, and the inhibitory effects of H-LSPC on serum MDA level, brain nNOS mRNA level and AchE activity. These results indicated that LC promoted the memory-enhancing effect of LSPC in scopolamine-induced amnesia mice.

Show MeSH
Related in: MedlinePlus