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N-3 PUFAs protect against aortic inflammation and oxidative stress in angiotensin II-infused apolipoprotein E-/- mice.

Wales KM, Kavazos K, Nataatmadja M, Brooks PR, Williams C, Russell FD - PLoS ONE (2014)

Bottom Line: The plasma concentration of the n-3 PUFA metabolite, resolvin D1 was higher in angiotensin II-infused ApoE-/- mice fed the high, compared to the low n-3 PUFA diet.Most neutrophils and macrophages were associated with dissected aortas.Immunoreactivity of the catalytic subunit of nicotinamide-adenine dinucleotide phosphate (NADPH) oxidase, Nox2, and superoxide were elevated in ApoE-/- mice that were fed the low n-3 PUFA diet, and this was also significantly attenuated in mice that were fed the high n-3 PUFA diet.

View Article: PubMed Central - PubMed

Affiliation: Inflammation and Healing Research Cluster, School of Health and Sport Sciences, University of the Sunshine Coast, Maroochydore, Queensland, Australia.

ABSTRACT
Abdominal aortic aneurysm is associated with infiltration of inflammatory cells into the aortic wall. The inflammatory response is also evident in animal models, such as apolipoprotein E-deficient (ApoE-/-) mice that have been infused with angiotensin II, prior to development of aortic aneurysm. Since omega-3 polyunsaturated fatty acids (n-3 PUFAs) and their metabolites have anti-inflammatory and pro-resolving activity, we hypothesised that dietary supplementation with n-3 PUFAs would protect against inflammatory processes in this mouse model. Twenty C57 and 20 ApoE-/- 3-4 week old male mice were supplemented with a low (0.14%, n = 10/group) or high (0.70%, n = 10/group) n-3 PUFA diet for 8 weeks before 2-day infusion with 0.9% saline or angiotensin II (1000 ng/kg/min). Four ApoE-/- mice on the low n-3 PUFA diet and none of the ApoE-/- mice on the high n-3 PUFA diet showed morphological evidence of abdominal aortic dissection. The plasma concentration of the n-3 PUFA metabolite, resolvin D1 was higher in angiotensin II-infused ApoE-/- mice fed the high, compared to the low n-3 PUFA diet. The number of neutrophils and macrophages infiltrating the abdominal aorta was elevated in ApoE-/- mice on the low n-3 PUFA diet, and this was significantly attenuated in mice that were fed the high n-3 PUFA diet. Most neutrophils and macrophages were associated with dissected aortas. Immunoreactivity of the catalytic subunit of nicotinamide-adenine dinucleotide phosphate (NADPH) oxidase, Nox2, and superoxide were elevated in ApoE-/- mice that were fed the low n-3 PUFA diet, and this was also significantly attenuated in mice that were fed the high n-3 PUFA diet. Together, the findings indicate that supplementation of ApoE-/- mice with a diet high in n-3 PUFA content protected the mice against pro-inflammatory and oxidative stress responses following short-term infusion with angiotensin II.

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Effect of dietary omega-3 polyunsaturated fatty acids (n-3 PUFAs) on mouse feeding behaviour, fatty acid incorporation in membrane phospholipids, and plasma resolvin D1 concentration.Mice were fed for 8 weeks with a low or high n-3 PUFA diet, and infused for 2 days with either saline (C57 mice) or angiotensin II (ApoE-/- mice). Amount of feed consumed (A), and mouse weight after 8-week dietary supplementation (B) were unaffected by diet. Total cholesterol was elevated in ApoE-/- mice, and this was attenuated by the high n-3 PUFA diet (C). The percentage of total fatty acids containing DHA (D), EPA (E), and DHA plus EPA (n-3 index; F) was significantly greater in mice receiving the high, compared to the low n-3 PUFA diet. The ratio of n-6 to n-3 PUFAs was lower in the animals receiving the high, compared to the low n-3 PUFA diet (G). The plasma concentration of resolvin D1 was higher in ApoE-/- mice receiving a high, compared to the low n-3 PUFA diet, with no difference in C57 mice (H). *, P<0.05. L, low n-3 PUFA diet; H, high n-3 PUFA diet.
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pone-0112816-g001: Effect of dietary omega-3 polyunsaturated fatty acids (n-3 PUFAs) on mouse feeding behaviour, fatty acid incorporation in membrane phospholipids, and plasma resolvin D1 concentration.Mice were fed for 8 weeks with a low or high n-3 PUFA diet, and infused for 2 days with either saline (C57 mice) or angiotensin II (ApoE-/- mice). Amount of feed consumed (A), and mouse weight after 8-week dietary supplementation (B) were unaffected by diet. Total cholesterol was elevated in ApoE-/- mice, and this was attenuated by the high n-3 PUFA diet (C). The percentage of total fatty acids containing DHA (D), EPA (E), and DHA plus EPA (n-3 index; F) was significantly greater in mice receiving the high, compared to the low n-3 PUFA diet. The ratio of n-6 to n-3 PUFAs was lower in the animals receiving the high, compared to the low n-3 PUFA diet (G). The plasma concentration of resolvin D1 was higher in ApoE-/- mice receiving a high, compared to the low n-3 PUFA diet, with no difference in C57 mice (H). *, P<0.05. L, low n-3 PUFA diet; H, high n-3 PUFA diet.

Mentions: Calculated data obtained from the manufacturer of the low n-3 PUFA diet showed ALA to be the main source of n-3 PUFAs, with EPA and DHA levels below the limit of detection (Table 1). The high n-3 PUFA diet contained more DHA than EPA. The mass of feed consumed, and mouse bodyweight for C57 and ApoE-/- mice was the same for animals that were fed the low and high n-3 PUFA diets (Figure 1A, B).


N-3 PUFAs protect against aortic inflammation and oxidative stress in angiotensin II-infused apolipoprotein E-/- mice.

Wales KM, Kavazos K, Nataatmadja M, Brooks PR, Williams C, Russell FD - PLoS ONE (2014)

Effect of dietary omega-3 polyunsaturated fatty acids (n-3 PUFAs) on mouse feeding behaviour, fatty acid incorporation in membrane phospholipids, and plasma resolvin D1 concentration.Mice were fed for 8 weeks with a low or high n-3 PUFA diet, and infused for 2 days with either saline (C57 mice) or angiotensin II (ApoE-/- mice). Amount of feed consumed (A), and mouse weight after 8-week dietary supplementation (B) were unaffected by diet. Total cholesterol was elevated in ApoE-/- mice, and this was attenuated by the high n-3 PUFA diet (C). The percentage of total fatty acids containing DHA (D), EPA (E), and DHA plus EPA (n-3 index; F) was significantly greater in mice receiving the high, compared to the low n-3 PUFA diet. The ratio of n-6 to n-3 PUFAs was lower in the animals receiving the high, compared to the low n-3 PUFA diet (G). The plasma concentration of resolvin D1 was higher in ApoE-/- mice receiving a high, compared to the low n-3 PUFA diet, with no difference in C57 mice (H). *, P<0.05. L, low n-3 PUFA diet; H, high n-3 PUFA diet.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4232505&req=5

pone-0112816-g001: Effect of dietary omega-3 polyunsaturated fatty acids (n-3 PUFAs) on mouse feeding behaviour, fatty acid incorporation in membrane phospholipids, and plasma resolvin D1 concentration.Mice were fed for 8 weeks with a low or high n-3 PUFA diet, and infused for 2 days with either saline (C57 mice) or angiotensin II (ApoE-/- mice). Amount of feed consumed (A), and mouse weight after 8-week dietary supplementation (B) were unaffected by diet. Total cholesterol was elevated in ApoE-/- mice, and this was attenuated by the high n-3 PUFA diet (C). The percentage of total fatty acids containing DHA (D), EPA (E), and DHA plus EPA (n-3 index; F) was significantly greater in mice receiving the high, compared to the low n-3 PUFA diet. The ratio of n-6 to n-3 PUFAs was lower in the animals receiving the high, compared to the low n-3 PUFA diet (G). The plasma concentration of resolvin D1 was higher in ApoE-/- mice receiving a high, compared to the low n-3 PUFA diet, with no difference in C57 mice (H). *, P<0.05. L, low n-3 PUFA diet; H, high n-3 PUFA diet.
Mentions: Calculated data obtained from the manufacturer of the low n-3 PUFA diet showed ALA to be the main source of n-3 PUFAs, with EPA and DHA levels below the limit of detection (Table 1). The high n-3 PUFA diet contained more DHA than EPA. The mass of feed consumed, and mouse bodyweight for C57 and ApoE-/- mice was the same for animals that were fed the low and high n-3 PUFA diets (Figure 1A, B).

Bottom Line: The plasma concentration of the n-3 PUFA metabolite, resolvin D1 was higher in angiotensin II-infused ApoE-/- mice fed the high, compared to the low n-3 PUFA diet.Most neutrophils and macrophages were associated with dissected aortas.Immunoreactivity of the catalytic subunit of nicotinamide-adenine dinucleotide phosphate (NADPH) oxidase, Nox2, and superoxide were elevated in ApoE-/- mice that were fed the low n-3 PUFA diet, and this was also significantly attenuated in mice that were fed the high n-3 PUFA diet.

View Article: PubMed Central - PubMed

Affiliation: Inflammation and Healing Research Cluster, School of Health and Sport Sciences, University of the Sunshine Coast, Maroochydore, Queensland, Australia.

ABSTRACT
Abdominal aortic aneurysm is associated with infiltration of inflammatory cells into the aortic wall. The inflammatory response is also evident in animal models, such as apolipoprotein E-deficient (ApoE-/-) mice that have been infused with angiotensin II, prior to development of aortic aneurysm. Since omega-3 polyunsaturated fatty acids (n-3 PUFAs) and their metabolites have anti-inflammatory and pro-resolving activity, we hypothesised that dietary supplementation with n-3 PUFAs would protect against inflammatory processes in this mouse model. Twenty C57 and 20 ApoE-/- 3-4 week old male mice were supplemented with a low (0.14%, n = 10/group) or high (0.70%, n = 10/group) n-3 PUFA diet for 8 weeks before 2-day infusion with 0.9% saline or angiotensin II (1000 ng/kg/min). Four ApoE-/- mice on the low n-3 PUFA diet and none of the ApoE-/- mice on the high n-3 PUFA diet showed morphological evidence of abdominal aortic dissection. The plasma concentration of the n-3 PUFA metabolite, resolvin D1 was higher in angiotensin II-infused ApoE-/- mice fed the high, compared to the low n-3 PUFA diet. The number of neutrophils and macrophages infiltrating the abdominal aorta was elevated in ApoE-/- mice on the low n-3 PUFA diet, and this was significantly attenuated in mice that were fed the high n-3 PUFA diet. Most neutrophils and macrophages were associated with dissected aortas. Immunoreactivity of the catalytic subunit of nicotinamide-adenine dinucleotide phosphate (NADPH) oxidase, Nox2, and superoxide were elevated in ApoE-/- mice that were fed the low n-3 PUFA diet, and this was also significantly attenuated in mice that were fed the high n-3 PUFA diet. Together, the findings indicate that supplementation of ApoE-/- mice with a diet high in n-3 PUFA content protected the mice against pro-inflammatory and oxidative stress responses following short-term infusion with angiotensin II.

Show MeSH
Related in: MedlinePlus