Limits...
Adipose hypothermia in obesity and its association with period homolog 1, insulin sensitivity, and inflammation in fat.

Yamaoka M, Maeda N, Takayama Y, Sekimoto R, Tsushima Y, Matsuda K, Mori T, Inoue K, Nishizawa H, Tominaga M, Funahashi T, Shimomura I - PLoS ONE (2014)

Bottom Line: Genes related to circadian rhythm were highly associated with visceral fat area and period homolog 1 (PER1) showed the most significant negative correlation with visceral fat area.However, no significant changes in Per1 mRNA level were observed by these agents.Exposure of cultured 3T3-L1 adipocytes to low temperature (33°C) decreased Per1 and catalase, and increased monocyte chemoattractant protein-1 (Mcp-1) mRNA levels.

View Article: PubMed Central - PubMed

Affiliation: Department of Metabolic Medicine, Graduate School of Medicine, Osaka University, Suita, Osaka, 565-0871, Japan.

ABSTRACT
Visceral fat adiposity plays an important role in the development of metabolic syndrome. We reported previously the impact of human visceral fat adiposity on gene expression profile of peripheral blood cells. Genes related to circadian rhythm were highly associated with visceral fat area and period homolog 1 (PER1) showed the most significant negative correlation with visceral fat area. However, regulation of adipose Per1 remains poorly understood. The present study was designed to understand the regulation of Per1 in adipose tissues. Adipose Per1 mRNA levels of ob/ob mice were markedly low at 25 and 35 weeks of age. The levels of other core clock genes of white adipose tissues were also low in ob/ob mice at 25 and 35 weeks of age. Per1 mRNA was mainly expressed in the mature adipocyte fraction (MAF) and it was significantly low in MAF of ob/ob mice. To examine the possible mechanisms, 3T3-L1 adipocytes were treated with H2O2, tumor necrosis factor-α (TNF-α), S100A8, and lipopolysaccharide (LPS). However, no significant changes in Per1 mRNA level were observed by these agents. Exposure of cultured 3T3-L1 adipocytes to low temperature (33°C) decreased Per1 and catalase, and increased monocyte chemoattractant protein-1 (Mcp-1) mRNA levels. Hypothermia also worsened insulin-mediated Akt phosphorylation in 3T3-L1 adipocytes. Finally, telemetric analysis showed low temperature of adipose tissues in ob/ob mice. In obesity, adipose hypothermia seems to accelerate adipocyte dysfunction.

Show MeSH

Related in: MedlinePlus

Core clock genes mRNA levels in obese adipose tissues.A, Tissue distribution of Per1 mRNA. The Per1 mRNA level of the eye was set at 1, and the mRNA levels of Per1 in other tissues are presented relative to the Per1 mRNA level of the eye. B to J, Changes in adipose core clock genes and adipose-related genes in C57BL/6N (B6) and ob/ob (ob) mice at 8, 25 and 35 weeks of age. n = 3–8 per group. Values are mean ± SEM. **P<0.01; ***P<0.001. $P<0.05; $$P<0.01; $$$P<0.001, compared to 8-week-old B6 mice. ##P<0.01; ###P<0.001, compared to 8-week-old ob mice.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4232416&req=5

pone-0112813-g001: Core clock genes mRNA levels in obese adipose tissues.A, Tissue distribution of Per1 mRNA. The Per1 mRNA level of the eye was set at 1, and the mRNA levels of Per1 in other tissues are presented relative to the Per1 mRNA level of the eye. B to J, Changes in adipose core clock genes and adipose-related genes in C57BL/6N (B6) and ob/ob (ob) mice at 8, 25 and 35 weeks of age. n = 3–8 per group. Values are mean ± SEM. **P<0.01; ***P<0.001. $P<0.05; $$P<0.01; $$$P<0.001, compared to 8-week-old B6 mice. ##P<0.01; ###P<0.001, compared to 8-week-old ob mice.

Mentions: Tissue distribution of Per1 was investigated in 8-week-old B6 mice (Figure 1A). Per1 was ubiquitously expressed in various tissues and its mRNA was abundantly expressed in WAT.


Adipose hypothermia in obesity and its association with period homolog 1, insulin sensitivity, and inflammation in fat.

Yamaoka M, Maeda N, Takayama Y, Sekimoto R, Tsushima Y, Matsuda K, Mori T, Inoue K, Nishizawa H, Tominaga M, Funahashi T, Shimomura I - PLoS ONE (2014)

Core clock genes mRNA levels in obese adipose tissues.A, Tissue distribution of Per1 mRNA. The Per1 mRNA level of the eye was set at 1, and the mRNA levels of Per1 in other tissues are presented relative to the Per1 mRNA level of the eye. B to J, Changes in adipose core clock genes and adipose-related genes in C57BL/6N (B6) and ob/ob (ob) mice at 8, 25 and 35 weeks of age. n = 3–8 per group. Values are mean ± SEM. **P<0.01; ***P<0.001. $P<0.05; $$P<0.01; $$$P<0.001, compared to 8-week-old B6 mice. ##P<0.01; ###P<0.001, compared to 8-week-old ob mice.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4232416&req=5

pone-0112813-g001: Core clock genes mRNA levels in obese adipose tissues.A, Tissue distribution of Per1 mRNA. The Per1 mRNA level of the eye was set at 1, and the mRNA levels of Per1 in other tissues are presented relative to the Per1 mRNA level of the eye. B to J, Changes in adipose core clock genes and adipose-related genes in C57BL/6N (B6) and ob/ob (ob) mice at 8, 25 and 35 weeks of age. n = 3–8 per group. Values are mean ± SEM. **P<0.01; ***P<0.001. $P<0.05; $$P<0.01; $$$P<0.001, compared to 8-week-old B6 mice. ##P<0.01; ###P<0.001, compared to 8-week-old ob mice.
Mentions: Tissue distribution of Per1 was investigated in 8-week-old B6 mice (Figure 1A). Per1 was ubiquitously expressed in various tissues and its mRNA was abundantly expressed in WAT.

Bottom Line: Genes related to circadian rhythm were highly associated with visceral fat area and period homolog 1 (PER1) showed the most significant negative correlation with visceral fat area.However, no significant changes in Per1 mRNA level were observed by these agents.Exposure of cultured 3T3-L1 adipocytes to low temperature (33°C) decreased Per1 and catalase, and increased monocyte chemoattractant protein-1 (Mcp-1) mRNA levels.

View Article: PubMed Central - PubMed

Affiliation: Department of Metabolic Medicine, Graduate School of Medicine, Osaka University, Suita, Osaka, 565-0871, Japan.

ABSTRACT
Visceral fat adiposity plays an important role in the development of metabolic syndrome. We reported previously the impact of human visceral fat adiposity on gene expression profile of peripheral blood cells. Genes related to circadian rhythm were highly associated with visceral fat area and period homolog 1 (PER1) showed the most significant negative correlation with visceral fat area. However, regulation of adipose Per1 remains poorly understood. The present study was designed to understand the regulation of Per1 in adipose tissues. Adipose Per1 mRNA levels of ob/ob mice were markedly low at 25 and 35 weeks of age. The levels of other core clock genes of white adipose tissues were also low in ob/ob mice at 25 and 35 weeks of age. Per1 mRNA was mainly expressed in the mature adipocyte fraction (MAF) and it was significantly low in MAF of ob/ob mice. To examine the possible mechanisms, 3T3-L1 adipocytes were treated with H2O2, tumor necrosis factor-α (TNF-α), S100A8, and lipopolysaccharide (LPS). However, no significant changes in Per1 mRNA level were observed by these agents. Exposure of cultured 3T3-L1 adipocytes to low temperature (33°C) decreased Per1 and catalase, and increased monocyte chemoattractant protein-1 (Mcp-1) mRNA levels. Hypothermia also worsened insulin-mediated Akt phosphorylation in 3T3-L1 adipocytes. Finally, telemetric analysis showed low temperature of adipose tissues in ob/ob mice. In obesity, adipose hypothermia seems to accelerate adipocyte dysfunction.

Show MeSH
Related in: MedlinePlus