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Sequencing, annotation and analysis of the Syrian hamster (Mesocricetus auratus) transcriptome.

Tchitchek N, Safronetz D, Rasmussen AL, Martens C, Virtaneva K, Porcella SF, Feldmann H, Ebihara H, Katze MG - PLoS ONE (2014)

Bottom Line: Sequence reads were assembled into 62,482 contigs and 111,796 reads remained unassembled (singletons).Canonical pathways involved in a broad spectrum of fundamental biological processes were significantly represented in the library.Furthermore, these data provide the basis for development of expression microarrays that can be used in functional genomics studies.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, University of Washington, Seattle, Washington, United States of America.

ABSTRACT

Background: The Syrian hamster (golden hamster, Mesocricetus auratus) is gaining importance as a new experimental animal model for multiple pathogens, including emerging zoonotic diseases such as Ebola. Nevertheless there are currently no publicly available transcriptome reference sequences or genome for this species.

Results: A cDNA library derived from mRNA and snRNA isolated and pooled from the brains, lungs, spleens, kidneys, livers, and hearts of three adult female Syrian hamsters was sequenced. Sequence reads were assembled into 62,482 contigs and 111,796 reads remained unassembled (singletons). This combined contig/singleton dataset, designated as the Syrian hamster transcriptome, represents a total of 60,117,204 nucleotides. Our Mesocricetus auratus Syrian hamster transcriptome mapped to 11,648 mouse transcripts representing 9,562 distinct genes, and mapped to a similar number of transcripts and genes in the rat. We identified 214 quasi-complete transcripts based on mouse annotations. Canonical pathways involved in a broad spectrum of fundamental biological processes were significantly represented in the library. The Syrian hamster transcriptome was aligned to the current release of the Chinese hamster ovary (CHO) cell transcriptome and genome to improve the genomic annotation of this species. Finally, our Syrian hamster transcriptome was aligned against 14 other rodents, primate and laurasiatheria species to gain insights about the genetic relatedness and placement of this species.

Conclusions: This Syrian hamster transcriptome dataset significantly improves our knowledge of the Syrian hamster's transcriptome, especially towards its future use in infectious disease research. Moreover, this library is an important resource for the wider scientific community to help improve genome annotation of the Syrian hamster and other closely related species. Furthermore, these data provide the basis for development of expression microarrays that can be used in functional genomics studies.

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Related in: MedlinePlus

Distogram showing the commonly mapped transcripts and phylogenetic tree showing the divergences amongst the different species.(A) Distogram showing the number of transcripts commonly mapped by the Syrian hamster transcriptome between the different species used in this study. Each cell of the distogram represents the number of transcripts commonly mapped by two different species using a gradient color. (B) Phylogenetic tree showing the genomic divergence between a subset of the different species used in this study. Each leaf of the tree represents a different species and the distances of the edges are proportional to the genomic distances between the species. Genomic distances have been calculated based on the list of 611 Syrian hamster contigs and singletons that have been commonly aligned on the transcriptome references of the 13 species having the highest number of commonly aligned sequences.
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pone-0112617-g004: Distogram showing the commonly mapped transcripts and phylogenetic tree showing the divergences amongst the different species.(A) Distogram showing the number of transcripts commonly mapped by the Syrian hamster transcriptome between the different species used in this study. Each cell of the distogram represents the number of transcripts commonly mapped by two different species using a gradient color. (B) Phylogenetic tree showing the genomic divergence between a subset of the different species used in this study. Each leaf of the tree represents a different species and the distances of the edges are proportional to the genomic distances between the species. Genomic distances have been calculated based on the list of 611 Syrian hamster contigs and singletons that have been commonly aligned on the transcriptome references of the 13 species having the highest number of commonly aligned sequences.

Mentions: Figure 4A is a distogram showing the results of our analysis of transcript sequences shared in common. The kangaroo rat, pika, shrew, and tree shrew had the lowest amount of commonly aligned sequences, amongst themselves and with the other species. The mouse and rat species showed the highest number of aligned sequences, presumably because of both their relatedness and genome quality/completeness.


Sequencing, annotation and analysis of the Syrian hamster (Mesocricetus auratus) transcriptome.

Tchitchek N, Safronetz D, Rasmussen AL, Martens C, Virtaneva K, Porcella SF, Feldmann H, Ebihara H, Katze MG - PLoS ONE (2014)

Distogram showing the commonly mapped transcripts and phylogenetic tree showing the divergences amongst the different species.(A) Distogram showing the number of transcripts commonly mapped by the Syrian hamster transcriptome between the different species used in this study. Each cell of the distogram represents the number of transcripts commonly mapped by two different species using a gradient color. (B) Phylogenetic tree showing the genomic divergence between a subset of the different species used in this study. Each leaf of the tree represents a different species and the distances of the edges are proportional to the genomic distances between the species. Genomic distances have been calculated based on the list of 611 Syrian hamster contigs and singletons that have been commonly aligned on the transcriptome references of the 13 species having the highest number of commonly aligned sequences.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4232415&req=5

pone-0112617-g004: Distogram showing the commonly mapped transcripts and phylogenetic tree showing the divergences amongst the different species.(A) Distogram showing the number of transcripts commonly mapped by the Syrian hamster transcriptome between the different species used in this study. Each cell of the distogram represents the number of transcripts commonly mapped by two different species using a gradient color. (B) Phylogenetic tree showing the genomic divergence between a subset of the different species used in this study. Each leaf of the tree represents a different species and the distances of the edges are proportional to the genomic distances between the species. Genomic distances have been calculated based on the list of 611 Syrian hamster contigs and singletons that have been commonly aligned on the transcriptome references of the 13 species having the highest number of commonly aligned sequences.
Mentions: Figure 4A is a distogram showing the results of our analysis of transcript sequences shared in common. The kangaroo rat, pika, shrew, and tree shrew had the lowest amount of commonly aligned sequences, amongst themselves and with the other species. The mouse and rat species showed the highest number of aligned sequences, presumably because of both their relatedness and genome quality/completeness.

Bottom Line: Sequence reads were assembled into 62,482 contigs and 111,796 reads remained unassembled (singletons).Canonical pathways involved in a broad spectrum of fundamental biological processes were significantly represented in the library.Furthermore, these data provide the basis for development of expression microarrays that can be used in functional genomics studies.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, University of Washington, Seattle, Washington, United States of America.

ABSTRACT

Background: The Syrian hamster (golden hamster, Mesocricetus auratus) is gaining importance as a new experimental animal model for multiple pathogens, including emerging zoonotic diseases such as Ebola. Nevertheless there are currently no publicly available transcriptome reference sequences or genome for this species.

Results: A cDNA library derived from mRNA and snRNA isolated and pooled from the brains, lungs, spleens, kidneys, livers, and hearts of three adult female Syrian hamsters was sequenced. Sequence reads were assembled into 62,482 contigs and 111,796 reads remained unassembled (singletons). This combined contig/singleton dataset, designated as the Syrian hamster transcriptome, represents a total of 60,117,204 nucleotides. Our Mesocricetus auratus Syrian hamster transcriptome mapped to 11,648 mouse transcripts representing 9,562 distinct genes, and mapped to a similar number of transcripts and genes in the rat. We identified 214 quasi-complete transcripts based on mouse annotations. Canonical pathways involved in a broad spectrum of fundamental biological processes were significantly represented in the library. The Syrian hamster transcriptome was aligned to the current release of the Chinese hamster ovary (CHO) cell transcriptome and genome to improve the genomic annotation of this species. Finally, our Syrian hamster transcriptome was aligned against 14 other rodents, primate and laurasiatheria species to gain insights about the genetic relatedness and placement of this species.

Conclusions: This Syrian hamster transcriptome dataset significantly improves our knowledge of the Syrian hamster's transcriptome, especially towards its future use in infectious disease research. Moreover, this library is an important resource for the wider scientific community to help improve genome annotation of the Syrian hamster and other closely related species. Furthermore, these data provide the basis for development of expression microarrays that can be used in functional genomics studies.

Show MeSH
Related in: MedlinePlus