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Comparative genomic analysis shows that avian pathogenic Escherichia coli isolate IMT5155 (O2:K1:H5; ST complex 95, ST140) shares close relationship with ST95 APEC O1:K1 and human ExPEC O18:K1 strains.

Zhu Ge X, Jiang J, Pan Z, Hu L, Wang S, Wang H, Leung FC, Dai J, Fan H - PLoS ONE (2014)

Bottom Line: Furthermore, the unique PAI I5155 (GI-12) was identified and found to be conserved in APEC O2 serotype isolates.The distribution analysis of 10 sequenced ExPEC pan-genome virulence factors among 47 sequenced E. coli strains provided meaningful information for B2 APEC/ExPEC-specific virulence factors, including several adhesins, invasins, toxins, iron acquisition systems, and so on.The pathogenicity tests of IMT5155 and other APEC O1:K1 and O2:K1 serotypes strains (isolated in China) through four animal models showed that they were highly virulent for avian colisepticemia and able to cause septicemia and meningitis in neonatal rats, suggesting zoonotic potential of these APEC O1:K1 and O2:K1 isolates.

View Article: PubMed Central - PubMed

Affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.

ABSTRACT
Avian pathogenic E. coli and human extraintestinal pathogenic E. coli serotypes O1, O2 and O18 strains isolated from different hosts are generally located in phylogroup B2 and ST complex 95, and they share similar genetic characteristics and pathogenicity, with no or minimal host specificity. They are popular objects for the study of ExPEC genetic characteristics and pathogenesis in recent years. Here, we investigated the evolution and genetic blueprint of APEC pathotype by performing phylogenetic and comparative genome analysis of avian pathogenic E. coli strain IMT5155 (O2:K1:H5; ST complex 95, ST140) with other E. coli pathotypes. Phylogeny analyses indicated that IMT5155 has closest evolutionary relationship with APEC O1, IHE3034, and UTI89. Comparative genomic analysis showed that IMT5155 and APEC O1 shared significant genetic overlap/similarities with human ExPEC dominant O18:K1 strains (IHE3034 and UTI89). Furthermore, the unique PAI I5155 (GI-12) was identified and found to be conserved in APEC O2 serotype isolates. GI-7 and GI-16 encoding two typical T6SSs in IMT5155 might be useful markers for the identification of ExPEC dominant serotypes (O1, O2, and O18) strains. IMT5155 contained a ColV plasmid p1ColV5155, which defined the APEC pathotype. The distribution analysis of 10 sequenced ExPEC pan-genome virulence factors among 47 sequenced E. coli strains provided meaningful information for B2 APEC/ExPEC-specific virulence factors, including several adhesins, invasins, toxins, iron acquisition systems, and so on. The pathogenicity tests of IMT5155 and other APEC O1:K1 and O2:K1 serotypes strains (isolated in China) through four animal models showed that they were highly virulent for avian colisepticemia and able to cause septicemia and meningitis in neonatal rats, suggesting zoonotic potential of these APEC O1:K1 and O2:K1 isolates.

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Phylogenomic tree (1,782 concatenated core genes, 1.61 Mb) of 47 E. coli strains.All MrBayes with the GTR+G+I substitution model (BMCMC) was used for the reconstruction of the phylogenomic tree. The chain length was set to 10,000,000 (1 sample/1000 generations). 47 E. coli strains clearly divided into monophyletically phylogroups (A, B1, B2, D, and E), and ST complex 95 strains were highlighted in phylogenomic tree. 47 E. coli genomes data was listed in File A in File S3.
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pone-0112048-g001: Phylogenomic tree (1,782 concatenated core genes, 1.61 Mb) of 47 E. coli strains.All MrBayes with the GTR+G+I substitution model (BMCMC) was used for the reconstruction of the phylogenomic tree. The chain length was set to 10,000,000 (1 sample/1000 generations). 47 E. coli strains clearly divided into monophyletically phylogroups (A, B1, B2, D, and E), and ST complex 95 strains were highlighted in phylogenomic tree. 47 E. coli genomes data was listed in File A in File S3.

Mentions: Whole-genome-derived phylogeny of common genomes can accurately illustrate evolutionary relationships among different commensal and pathogenic E. coli variants [49]. The genomes of IMT5155 and another 46 E. coli strains were selected for mapping the whole-genome evolutionary phylogeny, ranging from a commensal K12 strain, through intestinal pathogenic strains, to the highlighted extraintestinal pathogenic strains (Figure 1). MrBayes was used to construct a BMCMC phylogenetic tree to define the evolutionary phylogeny of 47 whole genome sequenced E. coli strains, based on E. coli common genes. The common genes identified from IMT5155 and the others 46 E. coli genomes comprised 1,782 genes and covered approximately 1.61 Mb. The result of phylogeny showed that 47 E. coli strains could be clearly divided into six monophyletic groups, which was similar to the whole-genome-based phylogeny by both Rasko and McNally et al. [26], [50] (Figure 1). In the phylogenetic tree, APEC strains IMT5155 and APEC O1 were located in B2 ExPEC cluster (Figure 1), and an APEC O78 strain χ7122 was located in B1 clade (Figure 1). The phylogenomic tree showed that ST complex 95 APEC dominant O1:K1 and O2:K1 serotypes strains (APEC O1 and IMT5155) have the closest evolutionary relationships with human ExPEC dominant O18:K1 (ST95 complex) strains (UTI89 and IHE3034).


Comparative genomic analysis shows that avian pathogenic Escherichia coli isolate IMT5155 (O2:K1:H5; ST complex 95, ST140) shares close relationship with ST95 APEC O1:K1 and human ExPEC O18:K1 strains.

Zhu Ge X, Jiang J, Pan Z, Hu L, Wang S, Wang H, Leung FC, Dai J, Fan H - PLoS ONE (2014)

Phylogenomic tree (1,782 concatenated core genes, 1.61 Mb) of 47 E. coli strains.All MrBayes with the GTR+G+I substitution model (BMCMC) was used for the reconstruction of the phylogenomic tree. The chain length was set to 10,000,000 (1 sample/1000 generations). 47 E. coli strains clearly divided into monophyletically phylogroups (A, B1, B2, D, and E), and ST complex 95 strains were highlighted in phylogenomic tree. 47 E. coli genomes data was listed in File A in File S3.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4232414&req=5

pone-0112048-g001: Phylogenomic tree (1,782 concatenated core genes, 1.61 Mb) of 47 E. coli strains.All MrBayes with the GTR+G+I substitution model (BMCMC) was used for the reconstruction of the phylogenomic tree. The chain length was set to 10,000,000 (1 sample/1000 generations). 47 E. coli strains clearly divided into monophyletically phylogroups (A, B1, B2, D, and E), and ST complex 95 strains were highlighted in phylogenomic tree. 47 E. coli genomes data was listed in File A in File S3.
Mentions: Whole-genome-derived phylogeny of common genomes can accurately illustrate evolutionary relationships among different commensal and pathogenic E. coli variants [49]. The genomes of IMT5155 and another 46 E. coli strains were selected for mapping the whole-genome evolutionary phylogeny, ranging from a commensal K12 strain, through intestinal pathogenic strains, to the highlighted extraintestinal pathogenic strains (Figure 1). MrBayes was used to construct a BMCMC phylogenetic tree to define the evolutionary phylogeny of 47 whole genome sequenced E. coli strains, based on E. coli common genes. The common genes identified from IMT5155 and the others 46 E. coli genomes comprised 1,782 genes and covered approximately 1.61 Mb. The result of phylogeny showed that 47 E. coli strains could be clearly divided into six monophyletic groups, which was similar to the whole-genome-based phylogeny by both Rasko and McNally et al. [26], [50] (Figure 1). In the phylogenetic tree, APEC strains IMT5155 and APEC O1 were located in B2 ExPEC cluster (Figure 1), and an APEC O78 strain χ7122 was located in B1 clade (Figure 1). The phylogenomic tree showed that ST complex 95 APEC dominant O1:K1 and O2:K1 serotypes strains (APEC O1 and IMT5155) have the closest evolutionary relationships with human ExPEC dominant O18:K1 (ST95 complex) strains (UTI89 and IHE3034).

Bottom Line: Furthermore, the unique PAI I5155 (GI-12) was identified and found to be conserved in APEC O2 serotype isolates.The distribution analysis of 10 sequenced ExPEC pan-genome virulence factors among 47 sequenced E. coli strains provided meaningful information for B2 APEC/ExPEC-specific virulence factors, including several adhesins, invasins, toxins, iron acquisition systems, and so on.The pathogenicity tests of IMT5155 and other APEC O1:K1 and O2:K1 serotypes strains (isolated in China) through four animal models showed that they were highly virulent for avian colisepticemia and able to cause septicemia and meningitis in neonatal rats, suggesting zoonotic potential of these APEC O1:K1 and O2:K1 isolates.

View Article: PubMed Central - PubMed

Affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.

ABSTRACT
Avian pathogenic E. coli and human extraintestinal pathogenic E. coli serotypes O1, O2 and O18 strains isolated from different hosts are generally located in phylogroup B2 and ST complex 95, and they share similar genetic characteristics and pathogenicity, with no or minimal host specificity. They are popular objects for the study of ExPEC genetic characteristics and pathogenesis in recent years. Here, we investigated the evolution and genetic blueprint of APEC pathotype by performing phylogenetic and comparative genome analysis of avian pathogenic E. coli strain IMT5155 (O2:K1:H5; ST complex 95, ST140) with other E. coli pathotypes. Phylogeny analyses indicated that IMT5155 has closest evolutionary relationship with APEC O1, IHE3034, and UTI89. Comparative genomic analysis showed that IMT5155 and APEC O1 shared significant genetic overlap/similarities with human ExPEC dominant O18:K1 strains (IHE3034 and UTI89). Furthermore, the unique PAI I5155 (GI-12) was identified and found to be conserved in APEC O2 serotype isolates. GI-7 and GI-16 encoding two typical T6SSs in IMT5155 might be useful markers for the identification of ExPEC dominant serotypes (O1, O2, and O18) strains. IMT5155 contained a ColV plasmid p1ColV5155, which defined the APEC pathotype. The distribution analysis of 10 sequenced ExPEC pan-genome virulence factors among 47 sequenced E. coli strains provided meaningful information for B2 APEC/ExPEC-specific virulence factors, including several adhesins, invasins, toxins, iron acquisition systems, and so on. The pathogenicity tests of IMT5155 and other APEC O1:K1 and O2:K1 serotypes strains (isolated in China) through four animal models showed that they were highly virulent for avian colisepticemia and able to cause septicemia and meningitis in neonatal rats, suggesting zoonotic potential of these APEC O1:K1 and O2:K1 isolates.

Show MeSH
Related in: MedlinePlus