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Characterization of arbuscular mycorrhizal fungus communities of Aquilaria crassna and Tectona grandis roots and soils in Thailand plantations.

Chaiyasen A, Young JP, Teaumroong N, Gavinlertvatana P, Lumyong S - PLoS ONE (2014)

Bottom Line: A total of 38 distinct terminal restriction fragments (TRFs) were found, 31 of which were shared between A. crassna and T. grandis.The Glomeraceae were found to be common in all study sites.Future inoculum production and utilization efforts can be directed toward the identified symbiotic associates of these valuable tree species to enhance reforestation efforts.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai, Thailand.

ABSTRACT
Aquilaria crassna Pierre ex Lec. and Tectona grandis Linn.f. are sources of resin-suffused agarwood and teak timber, respectively. This study investigated arbuscular mycorrhizal (AM) fungus community structure in roots and rhizosphere soils of A. crassna and T. grandis from plantations in Thailand to understand whether AM fungal communities present in roots and rhizosphere soils vary with host plant species and study sites. Terminal restriction fragment length polymorphism complemented with clone libraries revealed that AM fungal community composition in A. crassna and T. grandis were similar. A total of 38 distinct terminal restriction fragments (TRFs) were found, 31 of which were shared between A. crassna and T. grandis. AM fungal communities in T. grandis samples from different sites were similar, as were those in A. crassna. The estimated average minimum numbers of AM fungal taxa per sample in roots and soils of T. grandis were at least 1.89 vs. 2.55, respectively, and those of A. crassna were 2.85 vs. 2.33 respectively. The TRFs were attributed to Claroideoglomeraceae, Diversisporaceae, Gigasporaceae and Glomeraceae. The Glomeraceae were found to be common in all study sites. Specific AM taxa in roots and soils of T. grandis and A. crassna were not affected by host plant species and sample source (root vs. soil) but affected by collecting site. Future inoculum production and utilization efforts can be directed toward the identified symbiotic associates of these valuable tree species to enhance reforestation efforts.

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Related in: MedlinePlus

Occurrence of TRFs from roots and soils in (a) Tectona grandis and (b) Aquilaria crassna.Bars indicate the proportion of samples that yielded each TRF; dots indicate the average intensity of that fragment (± SEM) in those samples. The letters indicate the restriction enzyme involved in each fragment size, a: MboI, b: HinfI and c: Hsp92II.
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pone-0112591-g003: Occurrence of TRFs from roots and soils in (a) Tectona grandis and (b) Aquilaria crassna.Bars indicate the proportion of samples that yielded each TRF; dots indicate the average intensity of that fragment (± SEM) in those samples. The letters indicate the restriction enzyme involved in each fragment size, a: MboI, b: HinfI and c: Hsp92II.

Mentions: Nearly all of the distinct TRFs (31 out of 38) were found in both host plant species (Figure 3). There were some differences in AM fungal communities between T. grandis and A. crassna because the TRF 329c (TRFs are identified by their relative mobility and a code indicating the restriction enzyme that generated them: a: MboI, b: HinfI and c: Hsp92II) was not found in T. grandis, while 5 TRFs (135c, 141b, 158c, 176b, and 435b) were not found in A. crassna. Comparison of the population in roots and soils of T. grandis (Fig 3a) showed that 6 TRFs (135c, 158c, 176b, 181c, 435b and 438b) were found only in roots, while 141b and 281a were only found in soils. In A. crassna (Figure 3b), TRFs 176c, 181c and 438b were only found in root samples.


Characterization of arbuscular mycorrhizal fungus communities of Aquilaria crassna and Tectona grandis roots and soils in Thailand plantations.

Chaiyasen A, Young JP, Teaumroong N, Gavinlertvatana P, Lumyong S - PLoS ONE (2014)

Occurrence of TRFs from roots and soils in (a) Tectona grandis and (b) Aquilaria crassna.Bars indicate the proportion of samples that yielded each TRF; dots indicate the average intensity of that fragment (± SEM) in those samples. The letters indicate the restriction enzyme involved in each fragment size, a: MboI, b: HinfI and c: Hsp92II.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4232412&req=5

pone-0112591-g003: Occurrence of TRFs from roots and soils in (a) Tectona grandis and (b) Aquilaria crassna.Bars indicate the proportion of samples that yielded each TRF; dots indicate the average intensity of that fragment (± SEM) in those samples. The letters indicate the restriction enzyme involved in each fragment size, a: MboI, b: HinfI and c: Hsp92II.
Mentions: Nearly all of the distinct TRFs (31 out of 38) were found in both host plant species (Figure 3). There were some differences in AM fungal communities between T. grandis and A. crassna because the TRF 329c (TRFs are identified by their relative mobility and a code indicating the restriction enzyme that generated them: a: MboI, b: HinfI and c: Hsp92II) was not found in T. grandis, while 5 TRFs (135c, 141b, 158c, 176b, and 435b) were not found in A. crassna. Comparison of the population in roots and soils of T. grandis (Fig 3a) showed that 6 TRFs (135c, 158c, 176b, 181c, 435b and 438b) were found only in roots, while 141b and 281a were only found in soils. In A. crassna (Figure 3b), TRFs 176c, 181c and 438b were only found in root samples.

Bottom Line: A total of 38 distinct terminal restriction fragments (TRFs) were found, 31 of which were shared between A. crassna and T. grandis.The Glomeraceae were found to be common in all study sites.Future inoculum production and utilization efforts can be directed toward the identified symbiotic associates of these valuable tree species to enhance reforestation efforts.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai, Thailand.

ABSTRACT
Aquilaria crassna Pierre ex Lec. and Tectona grandis Linn.f. are sources of resin-suffused agarwood and teak timber, respectively. This study investigated arbuscular mycorrhizal (AM) fungus community structure in roots and rhizosphere soils of A. crassna and T. grandis from plantations in Thailand to understand whether AM fungal communities present in roots and rhizosphere soils vary with host plant species and study sites. Terminal restriction fragment length polymorphism complemented with clone libraries revealed that AM fungal community composition in A. crassna and T. grandis were similar. A total of 38 distinct terminal restriction fragments (TRFs) were found, 31 of which were shared between A. crassna and T. grandis. AM fungal communities in T. grandis samples from different sites were similar, as were those in A. crassna. The estimated average minimum numbers of AM fungal taxa per sample in roots and soils of T. grandis were at least 1.89 vs. 2.55, respectively, and those of A. crassna were 2.85 vs. 2.33 respectively. The TRFs were attributed to Claroideoglomeraceae, Diversisporaceae, Gigasporaceae and Glomeraceae. The Glomeraceae were found to be common in all study sites. Specific AM taxa in roots and soils of T. grandis and A. crassna were not affected by host plant species and sample source (root vs. soil) but affected by collecting site. Future inoculum production and utilization efforts can be directed toward the identified symbiotic associates of these valuable tree species to enhance reforestation efforts.

Show MeSH
Related in: MedlinePlus