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Silver wire amplifies the signaling mechanism for IL-1beta production more than silver submicroparticles in human monocytic THP-1 cells.

Jung HJ, Pak PJ, Park SH, Ju JE, Kim JS, Lee HS, Chung N - PLoS ONE (2014)

Bottom Line: Silver materials have been widely used in diverse fields.This trend was also observed with each step of the signaling mechanism for IL-1β production, which is a single pathway affiliated with ROS generation or lysosomal rupture or both, cathepsin B, caspase-1 (NALP3 inflammasome), and finally IL-1β production in THP-1 cells.All these results suggest that, for development of safe and effective silver materials, the shape or form of silver materials should be considered, especially for macrophage cell lines because epithelial cell lines are not overly sensitive to silver materials.

View Article: PubMed Central - PubMed

Affiliation: Department of Biosystems and Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, Korea.

ABSTRACT
Silver materials have been widely used in diverse fields. However, their toxicity and their mechanism, especially in different forms, have not been studied sufficiently. Thus, cytotoxicity, apoptosis, and interleukin-1beta (IL-1β) production were investigated using macrophage-like THP-1 cells in the presence of Ag microparticles (AgMPs, 2.7 µm), Ag submicroparticles (AgSMPs, 150 nm), and Ag wires (AgWs, 274 nm×5.3 µm). The levels of cytotoxicity, apoptosis, and IL-1β production by AgWs were higher than those by the other two AgSMPs and AgMPs. This trend was also observed with each step of the signaling mechanism for IL-1β production, which is a single pathway affiliated with ROS generation or lysosomal rupture or both, cathepsin B, caspase-1 (NALP3 inflammasome), and finally IL-1β production in THP-1 cells. All these results suggest that, for development of safe and effective silver materials, the shape or form of silver materials should be considered, especially for macrophage cell lines because epithelial cell lines are not overly sensitive to silver materials.

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Phase-contrast micrograph of THP-1 cells morphology in both the absence and presence of silver materials.PMA-primed THP-1 cells were treated with 100 µg/mL of silver materials for 24 h. The cells were photographed using a phase-contrast microscope to estimate overall morphology (400×).
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pone-0112256-g002: Phase-contrast micrograph of THP-1 cells morphology in both the absence and presence of silver materials.PMA-primed THP-1 cells were treated with 100 µg/mL of silver materials for 24 h. The cells were photographed using a phase-contrast microscope to estimate overall morphology (400×).

Mentions: To assess the cytotoxicity of silver materials on THP-1 cells, overall morphologies of PMA-primed THP-1 cells in both the absence and presence of different forms of silver materials were observed using a phase-contrast microscope (Fig. 2). Unlike control, the cells cultured with AgWs were more elongated or had more of a crushed morphology than those cultured with the other silver materials.


Silver wire amplifies the signaling mechanism for IL-1beta production more than silver submicroparticles in human monocytic THP-1 cells.

Jung HJ, Pak PJ, Park SH, Ju JE, Kim JS, Lee HS, Chung N - PLoS ONE (2014)

Phase-contrast micrograph of THP-1 cells morphology in both the absence and presence of silver materials.PMA-primed THP-1 cells were treated with 100 µg/mL of silver materials for 24 h. The cells were photographed using a phase-contrast microscope to estimate overall morphology (400×).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4232372&req=5

pone-0112256-g002: Phase-contrast micrograph of THP-1 cells morphology in both the absence and presence of silver materials.PMA-primed THP-1 cells were treated with 100 µg/mL of silver materials for 24 h. The cells were photographed using a phase-contrast microscope to estimate overall morphology (400×).
Mentions: To assess the cytotoxicity of silver materials on THP-1 cells, overall morphologies of PMA-primed THP-1 cells in both the absence and presence of different forms of silver materials were observed using a phase-contrast microscope (Fig. 2). Unlike control, the cells cultured with AgWs were more elongated or had more of a crushed morphology than those cultured with the other silver materials.

Bottom Line: Silver materials have been widely used in diverse fields.This trend was also observed with each step of the signaling mechanism for IL-1β production, which is a single pathway affiliated with ROS generation or lysosomal rupture or both, cathepsin B, caspase-1 (NALP3 inflammasome), and finally IL-1β production in THP-1 cells.All these results suggest that, for development of safe and effective silver materials, the shape or form of silver materials should be considered, especially for macrophage cell lines because epithelial cell lines are not overly sensitive to silver materials.

View Article: PubMed Central - PubMed

Affiliation: Department of Biosystems and Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, Korea.

ABSTRACT
Silver materials have been widely used in diverse fields. However, their toxicity and their mechanism, especially in different forms, have not been studied sufficiently. Thus, cytotoxicity, apoptosis, and interleukin-1beta (IL-1β) production were investigated using macrophage-like THP-1 cells in the presence of Ag microparticles (AgMPs, 2.7 µm), Ag submicroparticles (AgSMPs, 150 nm), and Ag wires (AgWs, 274 nm×5.3 µm). The levels of cytotoxicity, apoptosis, and IL-1β production by AgWs were higher than those by the other two AgSMPs and AgMPs. This trend was also observed with each step of the signaling mechanism for IL-1β production, which is a single pathway affiliated with ROS generation or lysosomal rupture or both, cathepsin B, caspase-1 (NALP3 inflammasome), and finally IL-1β production in THP-1 cells. All these results suggest that, for development of safe and effective silver materials, the shape or form of silver materials should be considered, especially for macrophage cell lines because epithelial cell lines are not overly sensitive to silver materials.

Show MeSH
Related in: MedlinePlus