Toward a comprehensive map of the effectors of rab GTPases.
Bottom Line: However, for many Rabs, few, if any, effectors have been identified; hence, their role remains unclear.For many Rabs, this revealed specific interactions with Drosophila orthologs of known effectors.In addition, we found numerous Rab-specific interactions with known components of membrane traffic as well as with diverse proteins not previously linked to organelles or having no known function.
Affiliation: MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge CB2 0QH, UK.Show MeSH
Related in: MedlinePlus
Mentions: Rab6 is widely conserved among eukaryotes. Localized to the trans-Golgi, it is involved in retrograde traffic from endosomes and possibly exit from the trans-Golgi network and retrograde trafficking from Golgi to ER. Known Rab6 effectors include the dynein adaptor BicaudalD; the lipid phosphatase OCRL1; the golgins TMF1, golgin-97, GCC88, and GCC185; the putative Rab GEF DENND5; and GORAB/SCYL1BP1 (Hutagalung and Novick, 2011). Drosophila orthologs of five of these proteins were found associated with high specificity to the Rab6 column, being BicD, CG3573 (OCRL1), CG33052 (GORAB), dGolgin-97, and dGCC88 (Figure 5A). The Rab6 column also bound specifically other components of membrane traffic and some proteins whose function is less clear.
Affiliation: MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge CB2 0QH, UK.