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Clinical evaluation of salivary periodontal pathogen levels by real-time polymerase chain reaction in patients before dental implant treatment.

Ito T, Yasuda M, Kaneko H, Sasaki H, Kato T, Yajima Y - Clin Oral Implants Res (2013)

Bottom Line: The levels of P. gingivalis and T. forsythia were significantly higher in mop group than in np group (P < 0.05).The levels of all periodontal pathogens tested except A. actinomycetemcomitans were significantly higher in sp group than in np group (P < 0.05).The detection levels of the periodontal pathogens targeted in saliva samples were correlated with the periodontal status.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral and Maxillo-Facial Implantology, Tokyo Dental College, Chiba, Japan.

No MeSH data available.


Related in: MedlinePlus

Sampling of saliva. Patients were required to chew gum for 5 min, after which 3–5 ml saliva was obtained.
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fig01: Sampling of saliva. Patients were required to chew gum for 5 min, after which 3–5 ml saliva was obtained.

Mentions: Each patient was required to chew gum for at least 5 min prior to collection of saliva samples. A total of 3–5 ml stimulated whole saliva was then collected in an empty, sterile, 50-ml test tube (Fig.1). The patients were instructed not to brush their teeth or eat for up to 1 h prior to sampling, which was performed between 9:30 and 11:30 AM. The whole saliva was stored at −20°C until processing.


Clinical evaluation of salivary periodontal pathogen levels by real-time polymerase chain reaction in patients before dental implant treatment.

Ito T, Yasuda M, Kaneko H, Sasaki H, Kato T, Yajima Y - Clin Oral Implants Res (2013)

Sampling of saliva. Patients were required to chew gum for 5 min, after which 3–5 ml saliva was obtained.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4232322&req=5

fig01: Sampling of saliva. Patients were required to chew gum for 5 min, after which 3–5 ml saliva was obtained.
Mentions: Each patient was required to chew gum for at least 5 min prior to collection of saliva samples. A total of 3–5 ml stimulated whole saliva was then collected in an empty, sterile, 50-ml test tube (Fig.1). The patients were instructed not to brush their teeth or eat for up to 1 h prior to sampling, which was performed between 9:30 and 11:30 AM. The whole saliva was stored at −20°C until processing.

Bottom Line: The levels of P. gingivalis and T. forsythia were significantly higher in mop group than in np group (P < 0.05).The levels of all periodontal pathogens tested except A. actinomycetemcomitans were significantly higher in sp group than in np group (P < 0.05).The detection levels of the periodontal pathogens targeted in saliva samples were correlated with the periodontal status.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral and Maxillo-Facial Implantology, Tokyo Dental College, Chiba, Japan.

No MeSH data available.


Related in: MedlinePlus