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Generation of a large volume of clinically relevant nanometre-sized ultra-high-molecular-weight polyethylene wear particles for cell culture studies.

Liu A, Ingham E, Fisher J, Tipper JL - Proc Inst Mech Eng H (2014)

Bottom Line: The results indicated that microbial contamination was absent and endotoxin levels were low and within acceptable limits for the pharmaceutical industry, when a six-station pin-on-plate wear simulator was used to generate ultra-high-molecular-weight polyethylene wear particles in a non-sterile environment.Different pore-sized polycarbonate filters were investigated to isolate nanometre-sized ultra-high-molecular-weight polyethylene wear particles from the wear test lubricants.The use of the filter sequence of 10, 1, 0.1, 0.1 and 0.015 µm pore sizes allowed successful isolation of ultra-high-molecular-weight polyethylene wear particles with a size range of < 100 nm, which was suitable for cell culture studies.

View Article: PubMed Central - PubMed

Affiliation: Institute of Medical and Biological Engineering, School of Mechanical Engineering, Faculty of Engineering, University of Leeds, Leeds, UK.

ABSTRACT
It has recently been shown that the wear of ultra-high-molecular-weight polyethylene in hip and knee prostheses leads to the generation of nanometre-sized particles, in addition to micron-sized particles. The biological activity of nanometre-sized ultra-high-molecular-weight polyethylene wear particles has not, however, previously been studied due to difficulties in generating sufficient volumes of nanometre-sized ultra-high-molecular-weight polyethylene wear particles suitable for cell culture studies. In this study, wear simulation methods were investigated to generate a large volume of endotoxin-free clinically relevant nanometre-sized ultra-high-molecular-weight polyethylene wear particles. Both single-station and six-station multidirectional pin-on-plate wear simulators were used to generate ultra-high-molecular-weight polyethylene wear particles under sterile and non-sterile conditions. Microbial contamination and endotoxin levels in the lubricants were determined. The results indicated that microbial contamination was absent and endotoxin levels were low and within acceptable limits for the pharmaceutical industry, when a six-station pin-on-plate wear simulator was used to generate ultra-high-molecular-weight polyethylene wear particles in a non-sterile environment. Different pore-sized polycarbonate filters were investigated to isolate nanometre-sized ultra-high-molecular-weight polyethylene wear particles from the wear test lubricants. The use of the filter sequence of 10, 1, 0.1, 0.1 and 0.015 µm pore sizes allowed successful isolation of ultra-high-molecular-weight polyethylene wear particles with a size range of < 100 nm, which was suitable for cell culture studies.

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Percentage number and the percentage area distributions as a function of size of nanometre-sized wear particles from three filter sequences: (a, b) filter sequence A, (c, d) filter sequence B and (e, f) filter sequence C.
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fig3-0954411914528308: Percentage number and the percentage area distributions as a function of size of nanometre-sized wear particles from three filter sequences: (a, b) filter sequence A, (c, d) filter sequence B and (e, f) filter sequence C.

Mentions: For particles isolated from filter sequence A, although 90.19% of the particles were less than 100 nm in size, particles with a size range of greater than 100 nm accounted for a high percentage area of particles (22.07%; Figure 3(a) and (b)). For particles from filter sequence B, the percentage number and the percentage area of particles in the size range above 100 nm were decreased to 0.84% and 0.54%, respectively (Figure 3(c) and (d)). For particles from filter sequence C, only 0.074% of the total number of the wear particles was greater than 100 nm in size (Figure 3(e)). The area percentage of the particles with a size greater than 100 nm was reduced to 0.048% (Figure 3(f)).


Generation of a large volume of clinically relevant nanometre-sized ultra-high-molecular-weight polyethylene wear particles for cell culture studies.

Liu A, Ingham E, Fisher J, Tipper JL - Proc Inst Mech Eng H (2014)

Percentage number and the percentage area distributions as a function of size of nanometre-sized wear particles from three filter sequences: (a, b) filter sequence A, (c, d) filter sequence B and (e, f) filter sequence C.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2 - License 3
Show All Figures
getmorefigures.php?uid=PMC4232278&req=5

fig3-0954411914528308: Percentage number and the percentage area distributions as a function of size of nanometre-sized wear particles from three filter sequences: (a, b) filter sequence A, (c, d) filter sequence B and (e, f) filter sequence C.
Mentions: For particles isolated from filter sequence A, although 90.19% of the particles were less than 100 nm in size, particles with a size range of greater than 100 nm accounted for a high percentage area of particles (22.07%; Figure 3(a) and (b)). For particles from filter sequence B, the percentage number and the percentage area of particles in the size range above 100 nm were decreased to 0.84% and 0.54%, respectively (Figure 3(c) and (d)). For particles from filter sequence C, only 0.074% of the total number of the wear particles was greater than 100 nm in size (Figure 3(e)). The area percentage of the particles with a size greater than 100 nm was reduced to 0.048% (Figure 3(f)).

Bottom Line: The results indicated that microbial contamination was absent and endotoxin levels were low and within acceptable limits for the pharmaceutical industry, when a six-station pin-on-plate wear simulator was used to generate ultra-high-molecular-weight polyethylene wear particles in a non-sterile environment.Different pore-sized polycarbonate filters were investigated to isolate nanometre-sized ultra-high-molecular-weight polyethylene wear particles from the wear test lubricants.The use of the filter sequence of 10, 1, 0.1, 0.1 and 0.015 µm pore sizes allowed successful isolation of ultra-high-molecular-weight polyethylene wear particles with a size range of < 100 nm, which was suitable for cell culture studies.

View Article: PubMed Central - PubMed

Affiliation: Institute of Medical and Biological Engineering, School of Mechanical Engineering, Faculty of Engineering, University of Leeds, Leeds, UK.

ABSTRACT
It has recently been shown that the wear of ultra-high-molecular-weight polyethylene in hip and knee prostheses leads to the generation of nanometre-sized particles, in addition to micron-sized particles. The biological activity of nanometre-sized ultra-high-molecular-weight polyethylene wear particles has not, however, previously been studied due to difficulties in generating sufficient volumes of nanometre-sized ultra-high-molecular-weight polyethylene wear particles suitable for cell culture studies. In this study, wear simulation methods were investigated to generate a large volume of endotoxin-free clinically relevant nanometre-sized ultra-high-molecular-weight polyethylene wear particles. Both single-station and six-station multidirectional pin-on-plate wear simulators were used to generate ultra-high-molecular-weight polyethylene wear particles under sterile and non-sterile conditions. Microbial contamination and endotoxin levels in the lubricants were determined. The results indicated that microbial contamination was absent and endotoxin levels were low and within acceptable limits for the pharmaceutical industry, when a six-station pin-on-plate wear simulator was used to generate ultra-high-molecular-weight polyethylene wear particles in a non-sterile environment. Different pore-sized polycarbonate filters were investigated to isolate nanometre-sized ultra-high-molecular-weight polyethylene wear particles from the wear test lubricants. The use of the filter sequence of 10, 1, 0.1, 0.1 and 0.015 µm pore sizes allowed successful isolation of ultra-high-molecular-weight polyethylene wear particles with a size range of < 100 nm, which was suitable for cell culture studies.

Show MeSH
Related in: MedlinePlus