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Rare-cell enrichment by a rapid, label-free, ultrasonic isopycnic technique for medical diagnostics.

Bourquin Y, Syed A, Reboud J, Ranford-Cartwright LC, Barrett MP, Cooper JM - Angew. Chem. Int. Ed. Engl. (2014)

Bottom Line: For malaria, we differentiate between infected and non-infected red blood cells in a fingerprick-sized drop of blood.We are able to achieve an enrichment of circulating cells infected by the ring stage of the parasite over nonparasitized red blood cells by between two and three orders of magnitude in less than 3 seconds (enabling detection at parasitemia levels as low as 0.0005%).In a second example, we also show that our methods can be used to enrich different cell types, concentrating Trypanosoma in blood at very low levels of infection, on disposable, low-cost chips.

View Article: PubMed Central - PubMed

Affiliation: Division of Biomedical Engineering, University of Glasgow, Glasgow, G12 8LT (UK).

No MeSH data available.


Related in: MedlinePlus

Characterization of the separation mechanism using 5 μm fluorescent beads: silica (red, ρ=2 g cm−3) and polystyrene (green, ρ=1.05 g cm−3). a) Flows induced by the SAW within the drop at the beginning of the process, when the beads are distributed in the liquid. b) After actuation, for ρf=1 g cm−3, both types of beads were concentrated in the center of the drop as shown in the micrograph (c). d) For ρf=1.160 g cm−3, the lighter polystyrene beads were distributed at the periphery (e). Scale bars: 300 μm.
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fig02: Characterization of the separation mechanism using 5 μm fluorescent beads: silica (red, ρ=2 g cm−3) and polystyrene (green, ρ=1.05 g cm−3). a) Flows induced by the SAW within the drop at the beginning of the process, when the beads are distributed in the liquid. b) After actuation, for ρf=1 g cm−3, both types of beads were concentrated in the center of the drop as shown in the micrograph (c). d) For ρf=1.160 g cm−3, the lighter polystyrene beads were distributed at the periphery (e). Scale bars: 300 μm.

Mentions: The technique relies upon controlling the shape of the acoustic field to generate a unique pattern of fluid streaming within the blood. Asymmetric actuation of the drop on the surface of the piezoelectric substrate with the SAW (Figure 1 a) causes a circular rotational motion, inducing secondary flows (Figure 2 a) in a manner similar to Batchelor flows12–15 (see Note 2 in the Supporting Information). Here we have used a slanted finger interdigitated electrode (SFIDT; Figure 1 a) to create a narrow path of propagation, providing the asymmetry in the propagation of the waves.16


Rare-cell enrichment by a rapid, label-free, ultrasonic isopycnic technique for medical diagnostics.

Bourquin Y, Syed A, Reboud J, Ranford-Cartwright LC, Barrett MP, Cooper JM - Angew. Chem. Int. Ed. Engl. (2014)

Characterization of the separation mechanism using 5 μm fluorescent beads: silica (red, ρ=2 g cm−3) and polystyrene (green, ρ=1.05 g cm−3). a) Flows induced by the SAW within the drop at the beginning of the process, when the beads are distributed in the liquid. b) After actuation, for ρf=1 g cm−3, both types of beads were concentrated in the center of the drop as shown in the micrograph (c). d) For ρf=1.160 g cm−3, the lighter polystyrene beads were distributed at the periphery (e). Scale bars: 300 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4232275&req=5

fig02: Characterization of the separation mechanism using 5 μm fluorescent beads: silica (red, ρ=2 g cm−3) and polystyrene (green, ρ=1.05 g cm−3). a) Flows induced by the SAW within the drop at the beginning of the process, when the beads are distributed in the liquid. b) After actuation, for ρf=1 g cm−3, both types of beads were concentrated in the center of the drop as shown in the micrograph (c). d) For ρf=1.160 g cm−3, the lighter polystyrene beads were distributed at the periphery (e). Scale bars: 300 μm.
Mentions: The technique relies upon controlling the shape of the acoustic field to generate a unique pattern of fluid streaming within the blood. Asymmetric actuation of the drop on the surface of the piezoelectric substrate with the SAW (Figure 1 a) causes a circular rotational motion, inducing secondary flows (Figure 2 a) in a manner similar to Batchelor flows12–15 (see Note 2 in the Supporting Information). Here we have used a slanted finger interdigitated electrode (SFIDT; Figure 1 a) to create a narrow path of propagation, providing the asymmetry in the propagation of the waves.16

Bottom Line: For malaria, we differentiate between infected and non-infected red blood cells in a fingerprick-sized drop of blood.We are able to achieve an enrichment of circulating cells infected by the ring stage of the parasite over nonparasitized red blood cells by between two and three orders of magnitude in less than 3 seconds (enabling detection at parasitemia levels as low as 0.0005%).In a second example, we also show that our methods can be used to enrich different cell types, concentrating Trypanosoma in blood at very low levels of infection, on disposable, low-cost chips.

View Article: PubMed Central - PubMed

Affiliation: Division of Biomedical Engineering, University of Glasgow, Glasgow, G12 8LT (UK).

No MeSH data available.


Related in: MedlinePlus