The Janus transcription factor HapX controls fungal adaptation to both iron starvation and iron excess.
Bottom Line: We further demonstrate that a HapX homodimer and the CCAAT-binding complex (CBC) cooperatively bind an evolutionary conserved DNA motif in a target promoter.The latter reveals the mode of discrimination between general CBC and specific HapX/CBC target genes.Collectively, our study uncovers a novel regulatory mechanism mediating both iron resistance and adaptation to iron starvation by the same transcription factor complex with activating and repressing functions depending on ambient iron availability.
Affiliation: Division of Molecular Biology, Biocenter, Innsbruck Medical University, Innsbruck, Austria.Show MeSH
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Mentions: To further characterize HapX domains, we generated A. fumigatus strains expressing different C-terminal hapX truncations (strains hapX464 – hapX158), here untagged, under the control of the endogenous promoter and integrated at the hapX locus in ΔhapX (Fig5D).
Affiliation: Division of Molecular Biology, Biocenter, Innsbruck Medical University, Innsbruck, Austria.