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Considerations in binding diblock copolymers on hydrophilic alginate beads for providing an immunoprotective membrane.

Spasojevic M, Bhujbal S, Paredes G, de Haan BJ, Schouten AJ, de Vos P - J Biomed Mater Res A (2013)

Bottom Line: In the present study, we describe the stepwise considerations for successful application of diblock copolymer of polyethylene glycol (PEG) and poly-L-lysine (PLL) on the surface of alginate beads.Shorter PLL blocks resulted in less stable capsules.Our results show that we can successfully apply block copolymers on the surface of hydrophilic alginate beads without interfering with the physicochemical properties.

View Article: PubMed Central - PubMed

Affiliation: Department of Polymer Chemistry, Zernike Institute for Advanced Materials, University of Groningen, 9747, AG Groningen, the Netherlands; Departments of Pathology and Laboratory Medicine, Section of Medical Biology, Division of Immunoendocrinology, University of Groningen, Hanzeplein 1, 9700, RB Groningen, The Netherlands.

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NF-κB activation of alginate beads, PEG454-b-PLL100 capsules and PLL capsules made of nonpurified and purified intermediate-G alginates. Impure alginate coated with PLL or PEG454-b-PLL100 was coincubated with THP-1 monocytes with a NF-κB reporter (left). The same procedure was applied with purified alginates (right). [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]
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fig06: NF-κB activation of alginate beads, PEG454-b-PLL100 capsules and PLL capsules made of nonpurified and purified intermediate-G alginates. Impure alginate coated with PLL or PEG454-b-PLL100 was coincubated with THP-1 monocytes with a NF-κB reporter (left). The same procedure was applied with purified alginates (right). [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]

Mentions: As shown in Figure 6, the diblock polymer PEG454-b-PLL100 capsules was not able to reduce the immunostimulatory effect of impurities present in alginate. Our observations suggest that the impurities are soluble and diffuse out of the capsules as we found no adhesion of the THP-1 cell line on the capsules thus suggesting that there was no direct interaction between the cells and impurities on the surface of the beads. A positive finding is that the diblock copolymer coated capsules with purified alginates do not provoke any response of the THP-1 cell line.


Considerations in binding diblock copolymers on hydrophilic alginate beads for providing an immunoprotective membrane.

Spasojevic M, Bhujbal S, Paredes G, de Haan BJ, Schouten AJ, de Vos P - J Biomed Mater Res A (2013)

NF-κB activation of alginate beads, PEG454-b-PLL100 capsules and PLL capsules made of nonpurified and purified intermediate-G alginates. Impure alginate coated with PLL or PEG454-b-PLL100 was coincubated with THP-1 monocytes with a NF-κB reporter (left). The same procedure was applied with purified alginates (right). [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4232034&req=5

fig06: NF-κB activation of alginate beads, PEG454-b-PLL100 capsules and PLL capsules made of nonpurified and purified intermediate-G alginates. Impure alginate coated with PLL or PEG454-b-PLL100 was coincubated with THP-1 monocytes with a NF-κB reporter (left). The same procedure was applied with purified alginates (right). [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]
Mentions: As shown in Figure 6, the diblock polymer PEG454-b-PLL100 capsules was not able to reduce the immunostimulatory effect of impurities present in alginate. Our observations suggest that the impurities are soluble and diffuse out of the capsules as we found no adhesion of the THP-1 cell line on the capsules thus suggesting that there was no direct interaction between the cells and impurities on the surface of the beads. A positive finding is that the diblock copolymer coated capsules with purified alginates do not provoke any response of the THP-1 cell line.

Bottom Line: In the present study, we describe the stepwise considerations for successful application of diblock copolymer of polyethylene glycol (PEG) and poly-L-lysine (PLL) on the surface of alginate beads.Shorter PLL blocks resulted in less stable capsules.Our results show that we can successfully apply block copolymers on the surface of hydrophilic alginate beads without interfering with the physicochemical properties.

View Article: PubMed Central - PubMed

Affiliation: Department of Polymer Chemistry, Zernike Institute for Advanced Materials, University of Groningen, 9747, AG Groningen, the Netherlands; Departments of Pathology and Laboratory Medicine, Section of Medical Biology, Division of Immunoendocrinology, University of Groningen, Hanzeplein 1, 9700, RB Groningen, The Netherlands.

Show MeSH
Related in: MedlinePlus