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Acceleration of periosteal bone formation by human basic fibroblast growth factor containing a collagen-binding domain from Clostridium histolyticum collagenase.

Uchida K, Matsushita O, Naruse K, Mima T, Nishi N, Hattori S, Ogura T, Inoue G, Tanaka K, Takaso M - J Biomed Mater Res A (2013)

Bottom Line: However, repeated dosing is required for sustained therapeutic effect as the efficacy of bFGF decreases rapidly following its diffusion from bone defect sites.The affinity of the fusion protein towards collagen and demineralized bone matrix (DBM) was also confirmed by collagen-binding assays.Taken together, these properties suggest that the CB-bFGF/collagen composite is a promising material for bone repair in the clinical setting.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedic Surgery, Kitasato University School of Medicine, 1-15-1 Minami-ku Kitasato, Kanagawa, Japan.

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3D micro-CT analysis of rat femurs two weeks after local injection. A: 3D micro-CT image, A-1: PBS, A-2: 0.58 nmol bFGF, A-3: 0.58 nmol CB-bFGF. Green color: new bone; brown color: existing bone. The scale bars indicate 1 mm. B: New bone area, and C: Bone mineral content after local injection of 0.58 nmol bFGF or CB-bFGF. Data are presented as the mean ± S.E. (n = 8). a: p < 0.05 compared with the control group. b: p < 0.05 compared with the dose-matched group. [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]
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fig02: 3D micro-CT analysis of rat femurs two weeks after local injection. A: 3D micro-CT image, A-1: PBS, A-2: 0.58 nmol bFGF, A-3: 0.58 nmol CB-bFGF. Green color: new bone; brown color: existing bone. The scale bars indicate 1 mm. B: New bone area, and C: Bone mineral content after local injection of 0.58 nmol bFGF or CB-bFGF. Data are presented as the mean ± S.E. (n = 8). a: p < 0.05 compared with the control group. b: p < 0.05 compared with the dose-matched group. [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]

Mentions: New bone volume and bone mineral content in the femurs of rats locally injected with 0.58 nmol bFGF and CB-bFGF were significantly higher than those in the PBS-injection group [Fig. 2(A–C)]. However, no differences in these parameters were detected between the bFGF and CB-bFGF injection groups.


Acceleration of periosteal bone formation by human basic fibroblast growth factor containing a collagen-binding domain from Clostridium histolyticum collagenase.

Uchida K, Matsushita O, Naruse K, Mima T, Nishi N, Hattori S, Ogura T, Inoue G, Tanaka K, Takaso M - J Biomed Mater Res A (2013)

3D micro-CT analysis of rat femurs two weeks after local injection. A: 3D micro-CT image, A-1: PBS, A-2: 0.58 nmol bFGF, A-3: 0.58 nmol CB-bFGF. Green color: new bone; brown color: existing bone. The scale bars indicate 1 mm. B: New bone area, and C: Bone mineral content after local injection of 0.58 nmol bFGF or CB-bFGF. Data are presented as the mean ± S.E. (n = 8). a: p < 0.05 compared with the control group. b: p < 0.05 compared with the dose-matched group. [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4232007&req=5

fig02: 3D micro-CT analysis of rat femurs two weeks after local injection. A: 3D micro-CT image, A-1: PBS, A-2: 0.58 nmol bFGF, A-3: 0.58 nmol CB-bFGF. Green color: new bone; brown color: existing bone. The scale bars indicate 1 mm. B: New bone area, and C: Bone mineral content after local injection of 0.58 nmol bFGF or CB-bFGF. Data are presented as the mean ± S.E. (n = 8). a: p < 0.05 compared with the control group. b: p < 0.05 compared with the dose-matched group. [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]
Mentions: New bone volume and bone mineral content in the femurs of rats locally injected with 0.58 nmol bFGF and CB-bFGF were significantly higher than those in the PBS-injection group [Fig. 2(A–C)]. However, no differences in these parameters were detected between the bFGF and CB-bFGF injection groups.

Bottom Line: However, repeated dosing is required for sustained therapeutic effect as the efficacy of bFGF decreases rapidly following its diffusion from bone defect sites.The affinity of the fusion protein towards collagen and demineralized bone matrix (DBM) was also confirmed by collagen-binding assays.Taken together, these properties suggest that the CB-bFGF/collagen composite is a promising material for bone repair in the clinical setting.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedic Surgery, Kitasato University School of Medicine, 1-15-1 Minami-ku Kitasato, Kanagawa, Japan.

Show MeSH
Related in: MedlinePlus