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Establishment and operation of a Good Manufacturing Practice-compliant allogeneic Epstein-Barr virus (EBV)-specific cytotoxic cell bank for the treatment of EBV-associated lymphoproliferative disease.

Vickers MA, Wilkie GM, Robinson N, Rivera N, Haque T, Crawford DH, Barry J, Fraser N, Turner DM, Robertson V, Dyer P, Flanagan P, Newlands HR, Campbell J, Turner ML - Br. J. Haematol. (2014)

Bottom Line: Conventional treatments for PTLD are often successful, but risk organ rejection and cause significant side effects.Both cases of EBV-associated non-haematopoietic sarcoma receiving cells failed to achieve complete remission.Thus, this third party donor-derived EBV-specific CTL cell bank can supply most patients with appropriately matched cells and most recipients have good outcomes.

View Article: PubMed Central - PubMed

Affiliation: Scottish National Blood Transfusion Service, Aberdeen, London, UK; University of Aberdeen, Aberdeen, London, UK.

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Related in: MedlinePlus

Human leucocyte antigen matching of donors with recipients. The number of matches at five human leucocyte antigen (HLA) loci are shown as histograms of the best matched prospective recipient–donor pairs. Matches that resulted in cytotoxic T lymphocyte infusions are shown in grey, those that did not in black. For two pairs, information was only available at HLA class I.
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fig02: Human leucocyte antigen matching of donors with recipients. The number of matches at five human leucocyte antigen (HLA) loci are shown as histograms of the best matched prospective recipient–donor pairs. Matches that resulted in cytotoxic T lymphocyte infusions are shown in grey, those that did not in black. For two pairs, information was only available at HLA class I.

Mentions: To date, 36 HLA matching requests have been processed and 32 allocation reviews completed. The minimum number of antigens shared for both HLA class I and class II combined was 2/10 and the maximum 9/10 (Fig2). For the CTLs infused, the minimum number of antigens shared was 3/10 and the maximum 9/10. Figure2 understates the degree of matching somewhat, as information was only available at class I loci for two of the pairs (neither patient received infusions). There was a median of three class I matches and two class II matches. The degree of matching for class II improved with time as the numbers within the bank built up, but this effect was not apparent for class I. Those that were issued had a slightly higher degree of matching (3·0 vs. 2·8 class I and 1·9 vs. 1·8 class II).


Establishment and operation of a Good Manufacturing Practice-compliant allogeneic Epstein-Barr virus (EBV)-specific cytotoxic cell bank for the treatment of EBV-associated lymphoproliferative disease.

Vickers MA, Wilkie GM, Robinson N, Rivera N, Haque T, Crawford DH, Barry J, Fraser N, Turner DM, Robertson V, Dyer P, Flanagan P, Newlands HR, Campbell J, Turner ML - Br. J. Haematol. (2014)

Human leucocyte antigen matching of donors with recipients. The number of matches at five human leucocyte antigen (HLA) loci are shown as histograms of the best matched prospective recipient–donor pairs. Matches that resulted in cytotoxic T lymphocyte infusions are shown in grey, those that did not in black. For two pairs, information was only available at HLA class I.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4232001&req=5

fig02: Human leucocyte antigen matching of donors with recipients. The number of matches at five human leucocyte antigen (HLA) loci are shown as histograms of the best matched prospective recipient–donor pairs. Matches that resulted in cytotoxic T lymphocyte infusions are shown in grey, those that did not in black. For two pairs, information was only available at HLA class I.
Mentions: To date, 36 HLA matching requests have been processed and 32 allocation reviews completed. The minimum number of antigens shared for both HLA class I and class II combined was 2/10 and the maximum 9/10 (Fig2). For the CTLs infused, the minimum number of antigens shared was 3/10 and the maximum 9/10. Figure2 understates the degree of matching somewhat, as information was only available at class I loci for two of the pairs (neither patient received infusions). There was a median of three class I matches and two class II matches. The degree of matching for class II improved with time as the numbers within the bank built up, but this effect was not apparent for class I. Those that were issued had a slightly higher degree of matching (3·0 vs. 2·8 class I and 1·9 vs. 1·8 class II).

Bottom Line: Conventional treatments for PTLD are often successful, but risk organ rejection and cause significant side effects.Both cases of EBV-associated non-haematopoietic sarcoma receiving cells failed to achieve complete remission.Thus, this third party donor-derived EBV-specific CTL cell bank can supply most patients with appropriately matched cells and most recipients have good outcomes.

View Article: PubMed Central - PubMed

Affiliation: Scottish National Blood Transfusion Service, Aberdeen, London, UK; University of Aberdeen, Aberdeen, London, UK.

Show MeSH
Related in: MedlinePlus