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Mycobacterium avium genotype is associated with the therapeutic response to lung infection.

Kikuchi T, Kobashi Y, Hirano T, Tode N, Santoso A, Tamada T, Fujimura S, Mitsuhashi Y, Honda Y, Nukiwa T, Kaku M, Watanabe A, Ichinose M - Clin. Microbiol. Infect. (2013)

Bottom Line: In phylogenetic analysis using the genotypic distance aggregated from 16-dimensional VNTR data, 59 M. avium isolates were divided into three clusters, which showed a nearly significant association with therapeutic responses (p 0.06).We then subjected the raw 16-dimensional VNTR data directly to principal component analysis, and identified the genetic features that were significantly associated with the therapeutic response (p <0.05).By further analysis of logistic regression with a stepwise variable-selection, we constructed the highest likelihood multivariate model, adjusted for age, to predict a therapeutic response, using VNTR data from only four minisatellite loci.

View Article: PubMed Central - PubMed

Affiliation: Department of Respiratory Medicine, Tohoku University Graduate School of Medicine, Sendai, Japan; Department of Respiratory Medicine, Tohoku University Hospital, Sendai, Japan.

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Phylogenetic analysis of Mycobacterium avium isolates from 59 subjects with lung infections. From all VNTR profile data shown in Fig.1, the Manhattan distance between each pair of isolates from subjects with responsive () or refractory disease () was calculated. The phylogenetic distribution was analysed by a neighbour-joining algorithm, and is shown together with subject numbers as a radial dendrogram. The three major branches of M. avium are designated clusters (a), (b) and (c). The scale bar indicates genetic distance.
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fig02: Phylogenetic analysis of Mycobacterium avium isolates from 59 subjects with lung infections. From all VNTR profile data shown in Fig.1, the Manhattan distance between each pair of isolates from subjects with responsive () or refractory disease () was calculated. The phylogenetic distribution was analysed by a neighbour-joining algorithm, and is shown together with subject numbers as a radial dendrogram. The three major branches of M. avium are designated clusters (a), (b) and (c). The scale bar indicates genetic distance.

Mentions: VNTR cluster is defined in Fig. 2.


Mycobacterium avium genotype is associated with the therapeutic response to lung infection.

Kikuchi T, Kobashi Y, Hirano T, Tode N, Santoso A, Tamada T, Fujimura S, Mitsuhashi Y, Honda Y, Nukiwa T, Kaku M, Watanabe A, Ichinose M - Clin. Microbiol. Infect. (2013)

Phylogenetic analysis of Mycobacterium avium isolates from 59 subjects with lung infections. From all VNTR profile data shown in Fig.1, the Manhattan distance between each pair of isolates from subjects with responsive () or refractory disease () was calculated. The phylogenetic distribution was analysed by a neighbour-joining algorithm, and is shown together with subject numbers as a radial dendrogram. The three major branches of M. avium are designated clusters (a), (b) and (c). The scale bar indicates genetic distance.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4231998&req=5

fig02: Phylogenetic analysis of Mycobacterium avium isolates from 59 subjects with lung infections. From all VNTR profile data shown in Fig.1, the Manhattan distance between each pair of isolates from subjects with responsive () or refractory disease () was calculated. The phylogenetic distribution was analysed by a neighbour-joining algorithm, and is shown together with subject numbers as a radial dendrogram. The three major branches of M. avium are designated clusters (a), (b) and (c). The scale bar indicates genetic distance.
Mentions: VNTR cluster is defined in Fig. 2.

Bottom Line: In phylogenetic analysis using the genotypic distance aggregated from 16-dimensional VNTR data, 59 M. avium isolates were divided into three clusters, which showed a nearly significant association with therapeutic responses (p 0.06).We then subjected the raw 16-dimensional VNTR data directly to principal component analysis, and identified the genetic features that were significantly associated with the therapeutic response (p <0.05).By further analysis of logistic regression with a stepwise variable-selection, we constructed the highest likelihood multivariate model, adjusted for age, to predict a therapeutic response, using VNTR data from only four minisatellite loci.

View Article: PubMed Central - PubMed

Affiliation: Department of Respiratory Medicine, Tohoku University Graduate School of Medicine, Sendai, Japan; Department of Respiratory Medicine, Tohoku University Hospital, Sendai, Japan.

Show MeSH
Related in: MedlinePlus