Mycobacterium avium genotype is associated with the therapeutic response to lung infection.
Bottom Line: In phylogenetic analysis using the genotypic distance aggregated from 16-dimensional VNTR data, 59 M. avium isolates were divided into three clusters, which showed a nearly significant association with therapeutic responses (p 0.06).We then subjected the raw 16-dimensional VNTR data directly to principal component analysis, and identified the genetic features that were significantly associated with the therapeutic response (p <0.05).By further analysis of logistic regression with a stepwise variable-selection, we constructed the highest likelihood multivariate model, adjusted for age, to predict a therapeutic response, using VNTR data from only four minisatellite loci.
Affiliation: Department of Respiratory Medicine, Tohoku University Graduate School of Medicine, Sendai, Japan; Department of Respiratory Medicine, Tohoku University Hospital, Sendai, Japan.Show MeSH
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Mentions: To evaluate the association of mycobacterial genotype with therapeutic response, we obtained the VNTR profiling data of all M. avium isolates, determining the numbers of tandemly repeated motifs at minisatellite loci in the genome (Fig.1). The VNTR profile of M. avium isolated from each subject consists of 16 integer values from 16 minisatellite loci, MATR-1 to -16; subjects 1–30 had responsive M. avium lung disease, while subjects 31–59 had refractory disease. The 16 × 59 seemingly unrelated data points permit further evaluation of the association between mycobacterial genotype and the clinical response to anti-mycobacterial treatment.
Affiliation: Department of Respiratory Medicine, Tohoku University Graduate School of Medicine, Sendai, Japan; Department of Respiratory Medicine, Tohoku University Hospital, Sendai, Japan.