Shiga toxin production and translocation during microaerobic human colonic infection with Shiga toxin-producing E. coli O157:H7 and O104:H4.
Bottom Line: Haemolytic uraemic syndrome caused by Shiga toxin-producing E. coli (STEC) is dependent on release of Shiga toxins (Stxs) during intestinal infection and subsequent absorption into the bloodstream.An understanding of Stx-related events in the human gut is limited due to lack of suitable experimental models.Whereas microaerobiosis significantly reduced bacterial growth as well as Stx production and release into the medium, Stx translocation across the epithelial monolayer was enhanced under MA versus aerobic conditions.
Affiliation: Norwich Medical School, University of East Anglia, Norwich Research Park, Norwich, UK; Gut Health and Food Safety Programme, Institute of Food Research, Norwich Research Park, Norwich, UK.Show MeSH
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Mentions: Experiments to study the effect of oxygen on bacterial growth and Stx2 release were extended to O157:H7 strain Walla-1 and O104:H4 strain H1121. Both strains produce the same Stx2 subtype as EDL933 (Stx2a) but do not produce Stx1. Similar to EDL933, both strains showed significantly reduced growth and Stx2 supernatant levels under MA versus AE conditions (Fig. 6A and B). In addition, aerobic growth of H1121 was significantly lower compared with EDL933 (Fig. 6A) and Stx2 release by Walla-1 and H1121 was significantly reduced compared with EDL933 under AE and MA conditions (Fig. 6B).
Affiliation: Norwich Medical School, University of East Anglia, Norwich Research Park, Norwich, UK; Gut Health and Food Safety Programme, Institute of Food Research, Norwich Research Park, Norwich, UK.