Allosteric control of the exportin CRM1 unraveled by crystal structure analysis.
Bottom Line: Moreover, during the last decade, CRM1 has become a more and more appreciated target for anti-cancer drugs.Hence, detailed understanding of the flexibility, the regulatory features and the positive binding cooperativity between CRM1, Ran and cargo is a prerequisite for the development of highly effective drugs.Here we review recent structural advances in the characterization of CRM1 and CRM1-containing complexes with a special emphasis on X-ray crystallographic studies.
Affiliation: Abteilung für Molekulare Strukturbiologie, Institut für Mikrobiologie und Genetik, Göttinger Zentrum für Molekulare Biowissenschaften, Georg-August-Universität Göttingen, Germany.Show MeSH
Related in: MedlinePlus
Mentions: Generally, importin β type nuclear transport receptors are composed of a varying number of HEAT repeats. The canonical HEAT repeat is a structural motif formed by two anti-parallel helices (named A- and B-helix) connected by a short linker loop and a typical total length of ∼ 40–50 residues (Fig.2A) 77,78. They have been named after the first couple of proteins bearing this motif, namely huntingtin, elongation factor 3 (EF3), the regulatory A subunit of protein phosphatase 2A (PP2A) and the P3 kinase TOR1.
Affiliation: Abteilung für Molekulare Strukturbiologie, Institut für Mikrobiologie und Genetik, Göttinger Zentrum für Molekulare Biowissenschaften, Georg-August-Universität Göttingen, Germany.