THOC5 controls 3'end-processing of immediate early genes via interaction with polyadenylation specific factor 100 (CPSF100).
Bottom Line: Furthermore, THOC5 depletion does not influence the expression of the most rapidly induced IEGs, e.g. Fos and Jun.THOC5 is required for recruitment of CPSF100 to 3'UTR of THOC5 target genes.These data suggest the presence of a novel mechanism for the control of IEG response by THOC5 via 3'end-processing.
Affiliation: Institut fuer Biochemie, OE4310, Medizinische Hochschule Hannover, Carl-Neuberg-Strasse 1, D-30623 Hannover, Germany.Show MeSH
Mentions: We have previously shown that THOC5 is recruited to the last exon, but not the promoter region of the Ets gene induced by stimulation with macrophage-colony stimulating factor (M-CSF) (12). In agreement with these data, after stimulation with serum THOC5 was recruited to 3′UTR of the Id3 gene, but not the promoter region of Id3 (Figure 6A) (24). THOC5 was not recruited to the THOC5-independent IEG, Ier2 (Figure 6A). In control cells, CPSF100 was recruited to 3′UTR of the Id3 and Ier2 genes. In THOC5-depleted cells, CPSF100 was recruited to the Ier2 gene to a similar extent as in control cells, while it was not recruited to the Id3 gene (Figure 6B), suggesting that THOC5 plays a role in recruitment of CPSF100 to 3′UTR of its target gene. RNA polymerase II was equally recruited to both genes in the presence or absence of THOC5 (Figure 6C), suggesting that THOC5 participates in 3′end-processing, but not elongation of THOC5 target genes.
Affiliation: Institut fuer Biochemie, OE4310, Medizinische Hochschule Hannover, Carl-Neuberg-Strasse 1, D-30623 Hannover, Germany.