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Homodimerization of HYL1 ensures the correct selection of cleavage sites in primary miRNA.

Yang X, Ren W, Zhao Q, Zhang P, Wu F, He Y - Nucleic Acids Res. (2014)

Bottom Line: Disruption of HYL1 homodimerization causes incorrect cleavage at sites in pri-miRNA without interrupting the interaction of HYL1 with DCL1 and accumulation of pri-miRNAs in HYL1/pri-miRNA complexes, leading to a reduction in the efficiency and accuracy of miRNAs that results in strong mutant phenotypes of the plants.HYL1 homodimers may function as a molecular anchor for DCL1 to cleave at a distance from the ssRNA-dsRNA junction in pri-miRNA.These results suggest that HYL1 ensures the correct selection of pri-miRNA cleavage sites through homodimerization and thus contributes to gene silencing and plant development.

View Article: PubMed Central - PubMed

Affiliation: National Key Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China.

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Model of how correct selection of pri-miRNA cleavage sites is affected by the absence of HYL1 and disruption of HYL1 dimerization. HYL1 promotes the correct selection of pri-miRNA cleavage sites through interaction with DCL1 and SE. The absence of HYL1 causes the incorrect selection of pri-miRNA cleavage sites mainly in the ssRNA regions, while disruption of HYL1 homodimerization results in the incorrect selection of pri-miRNA cleavage sites mainly in stem because pri-miRNA cleavage sites are incorrectly chosen. Wavy lines in red and blue indicate accurate miRNAs and miRNAs*, respectively. The lines in black show the incorrect ones, and the lines in mosaic red and black display the partially accurate ones.
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Figure 9: Model of how correct selection of pri-miRNA cleavage sites is affected by the absence of HYL1 and disruption of HYL1 dimerization. HYL1 promotes the correct selection of pri-miRNA cleavage sites through interaction with DCL1 and SE. The absence of HYL1 causes the incorrect selection of pri-miRNA cleavage sites mainly in the ssRNA regions, while disruption of HYL1 homodimerization results in the incorrect selection of pri-miRNA cleavage sites mainly in stem because pri-miRNA cleavage sites are incorrectly chosen. Wavy lines in red and blue indicate accurate miRNAs and miRNAs*, respectively. The lines in black show the incorrect ones, and the lines in mosaic red and black display the partially accurate ones.

Mentions: The model supposed in Figure 9 suggests that disruption of HYL1 homodimerization impairs the correct selection of cleavage sites in pri-miRNA because the distance from ssRNA–dsRNA junction is altered. The mechanisms by which the correct selection of cleavage sites occurs in pri- and/or pre-miRNA have remained a mystery. Nevertheless, our results indicate that this process may be dependent on HYL1 binding to the correct sites on pri-miRNA or pre-miRNAs. Further study on the binding sites of HYL1 on pri-miRNAs and pre-miRNAs will facilitate an improved understanding of the molecular mechanisms underlying miRNA biogenesis.


Homodimerization of HYL1 ensures the correct selection of cleavage sites in primary miRNA.

Yang X, Ren W, Zhao Q, Zhang P, Wu F, He Y - Nucleic Acids Res. (2014)

Model of how correct selection of pri-miRNA cleavage sites is affected by the absence of HYL1 and disruption of HYL1 dimerization. HYL1 promotes the correct selection of pri-miRNA cleavage sites through interaction with DCL1 and SE. The absence of HYL1 causes the incorrect selection of pri-miRNA cleavage sites mainly in the ssRNA regions, while disruption of HYL1 homodimerization results in the incorrect selection of pri-miRNA cleavage sites mainly in stem because pri-miRNA cleavage sites are incorrectly chosen. Wavy lines in red and blue indicate accurate miRNAs and miRNAs*, respectively. The lines in black show the incorrect ones, and the lines in mosaic red and black display the partially accurate ones.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4231765&req=5

Figure 9: Model of how correct selection of pri-miRNA cleavage sites is affected by the absence of HYL1 and disruption of HYL1 dimerization. HYL1 promotes the correct selection of pri-miRNA cleavage sites through interaction with DCL1 and SE. The absence of HYL1 causes the incorrect selection of pri-miRNA cleavage sites mainly in the ssRNA regions, while disruption of HYL1 homodimerization results in the incorrect selection of pri-miRNA cleavage sites mainly in stem because pri-miRNA cleavage sites are incorrectly chosen. Wavy lines in red and blue indicate accurate miRNAs and miRNAs*, respectively. The lines in black show the incorrect ones, and the lines in mosaic red and black display the partially accurate ones.
Mentions: The model supposed in Figure 9 suggests that disruption of HYL1 homodimerization impairs the correct selection of cleavage sites in pri-miRNA because the distance from ssRNA–dsRNA junction is altered. The mechanisms by which the correct selection of cleavage sites occurs in pri- and/or pre-miRNA have remained a mystery. Nevertheless, our results indicate that this process may be dependent on HYL1 binding to the correct sites on pri-miRNA or pre-miRNAs. Further study on the binding sites of HYL1 on pri-miRNAs and pre-miRNAs will facilitate an improved understanding of the molecular mechanisms underlying miRNA biogenesis.

Bottom Line: Disruption of HYL1 homodimerization causes incorrect cleavage at sites in pri-miRNA without interrupting the interaction of HYL1 with DCL1 and accumulation of pri-miRNAs in HYL1/pri-miRNA complexes, leading to a reduction in the efficiency and accuracy of miRNAs that results in strong mutant phenotypes of the plants.HYL1 homodimers may function as a molecular anchor for DCL1 to cleave at a distance from the ssRNA-dsRNA junction in pri-miRNA.These results suggest that HYL1 ensures the correct selection of pri-miRNA cleavage sites through homodimerization and thus contributes to gene silencing and plant development.

View Article: PubMed Central - PubMed

Affiliation: National Key Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China.

Show MeSH
Related in: MedlinePlus