Limits...
RAD51B plays an essential role during somatic and meiotic recombination in Physcomitrella.

Charlot F, Chelysheva L, Kamisugi Y, Vrielynck N, Guyon A, Epert A, Le Guin S, Schaefer DG, Cuming AC, Grelon M, Nogué F - Nucleic Acids Res. (2014)

Bottom Line: Here, we examined the role of RAD51B in the model bryophyte Physcomitrella patens.Mutant analysis shows that RAD51B is essential for the maintenance of genome integrity, for resistance to DNA damaging agents and for gene targeting.Finally, we show that all these functions are independent of the SRS2 anti-recombinase protein, which is in striking contrast to what is found in budding yeast where the RAD51 paralogues are fully dependent on the SRS2 anti-recombinase function.

View Article: PubMed Central - PubMed

Affiliation: INRA, Institut Jean-Pierre Bourgin UMR1318, Saclay Plant Sciences, Versailles, France AgroParisTech, Institut Jean-Pierre Bourgin UMR1318, Saclay Plant Sciences, Versailles, France.

Show MeSH

Related in: MedlinePlus

Vegetative development of the rad51bΔ mutants. Morphology of colonies and gametophores of WT (A, B), double rad51-1-2? mutant (C, D), two independent subclones of a rad51bΔ mutant (E, F and G, H) and srs2? mutant (I, J). The picture was taken after 30 days of growth. Scale bar = 5 mm.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4231755&req=5

Figure 3: Vegetative development of the rad51bΔ mutants. Morphology of colonies and gametophores of WT (A, B), double rad51-1-2? mutant (C, D), two independent subclones of a rad51bΔ mutant (E, F and G, H) and srs2? mutant (I, J). The picture was taken after 30 days of growth. Scale bar = 5 mm.

Mentions: The phenotype of the rad51bΔ mutants was characterized throughout this entire life cycle. By contrast with the double rad51 mutant already described (41,47), that shows a strong developmental phenotype on rich medium (Figure 3C and Supplementary Figure S2), the rad51bΔ mutants display only a very weak defect in gametophytic development (Figure 3E and 3F), showing a normal protonema development but fewer gametophores than WT (81% of the WT, see Figure 3A and Supplementary Figure S2). However, after multiple subcultures we occasionally observed some individuals showing strong developmental phenotypes (see one example in Figure 3G and 3H) suggesting the possibility of a genetic instability in mutant lines.


RAD51B plays an essential role during somatic and meiotic recombination in Physcomitrella.

Charlot F, Chelysheva L, Kamisugi Y, Vrielynck N, Guyon A, Epert A, Le Guin S, Schaefer DG, Cuming AC, Grelon M, Nogué F - Nucleic Acids Res. (2014)

Vegetative development of the rad51bΔ mutants. Morphology of colonies and gametophores of WT (A, B), double rad51-1-2? mutant (C, D), two independent subclones of a rad51bΔ mutant (E, F and G, H) and srs2? mutant (I, J). The picture was taken after 30 days of growth. Scale bar = 5 mm.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4231755&req=5

Figure 3: Vegetative development of the rad51bΔ mutants. Morphology of colonies and gametophores of WT (A, B), double rad51-1-2? mutant (C, D), two independent subclones of a rad51bΔ mutant (E, F and G, H) and srs2? mutant (I, J). The picture was taken after 30 days of growth. Scale bar = 5 mm.
Mentions: The phenotype of the rad51bΔ mutants was characterized throughout this entire life cycle. By contrast with the double rad51 mutant already described (41,47), that shows a strong developmental phenotype on rich medium (Figure 3C and Supplementary Figure S2), the rad51bΔ mutants display only a very weak defect in gametophytic development (Figure 3E and 3F), showing a normal protonema development but fewer gametophores than WT (81% of the WT, see Figure 3A and Supplementary Figure S2). However, after multiple subcultures we occasionally observed some individuals showing strong developmental phenotypes (see one example in Figure 3G and 3H) suggesting the possibility of a genetic instability in mutant lines.

Bottom Line: Here, we examined the role of RAD51B in the model bryophyte Physcomitrella patens.Mutant analysis shows that RAD51B is essential for the maintenance of genome integrity, for resistance to DNA damaging agents and for gene targeting.Finally, we show that all these functions are independent of the SRS2 anti-recombinase protein, which is in striking contrast to what is found in budding yeast where the RAD51 paralogues are fully dependent on the SRS2 anti-recombinase function.

View Article: PubMed Central - PubMed

Affiliation: INRA, Institut Jean-Pierre Bourgin UMR1318, Saclay Plant Sciences, Versailles, France AgroParisTech, Institut Jean-Pierre Bourgin UMR1318, Saclay Plant Sciences, Versailles, France.

Show MeSH
Related in: MedlinePlus