Quantitative effect of target translation on small RNA efficacy reveals a novel mode of interaction.
Bottom Line: Often the sRNA-binding site is adjacent to or overlapping with the ribosomal binding site (RBS), suggesting a possible interplay between sRNA and ribosome binding.Quantitative analysis of these data suggests a recruitment model, where bound ribosomes facilitate binding of the sRNA.Our findings offer a framework for understanding sRNA silencing in the context of bacterial physiology.
Affiliation: Department of Physics and FAS Center for Systems Biology, Harvard University, Cambridge, MA 02138, USA.Show MeSH
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Mentions: Model predictions plotted in Figures 2 and 5A were calculated using typical parameter values of bacterial sRNA pathways (27). Fixed parameters are (in min−1) .
Affiliation: Department of Physics and FAS Center for Systems Biology, Harvard University, Cambridge, MA 02138, USA.