Paramecium tetraurelia chromatin assembly factor-1-like protein PtCAF-1 is involved in RNA-mediated control of DNA elimination.
Bottom Line: Gene silencing shows that PtCAF-1 is required for the elimination of transposable elements and a subset of IESs.PTCAF-1 depletion also impairs the selection of germline-specific scnRNAs during development.We identify specific histone modifications appearing during Paramecium development which are strongly reduced in PTCAF-1 depleted cells.
Affiliation: Institute of Cell Biology, University of Bern, Baltzerstrasse 4, 3012 Bern, Switzerland Graduate School for Cellular and Biomedical Sciences, University of Bern, Freiestrasse 1, 3012 Bern, Switzerland.Show MeSH
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Mentions: To determine the localization of the PtCAF-1 protein during development, P. tetraurelia was transformed with an N-terminal GFP tagged PTCAF-1 set under the control of a sequence region containing the putative endogenous PTCAF-1 promoter. The localization of PtCAF-1 GFP fusion protein was observed at distinct stages during autogamy by fluorescence microscopy (Figure 3). No GFP signal was detectable during vegetative growth. Upon meiosis the GFP fusion protein was expressed and localized in the parental MAC where it remained during skein formation and in the separated fragments. Later in development the fusion protein accumulated in the new MACs where it persisted throughout MAC development. It was still detectable in the new MACs after karyonidal division. The PtCAF-1 GFP signal decreased with the maturation of the developing MACs. This localization pattern strongly resembles that of Nowa1 (27) reinforcing the hypothesis of PtCAF-1 being a factor in genome rearrangements of P. tetraurelia.
Affiliation: Institute of Cell Biology, University of Bern, Baltzerstrasse 4, 3012 Bern, Switzerland Graduate School for Cellular and Biomedical Sciences, University of Bern, Freiestrasse 1, 3012 Bern, Switzerland.