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ADAR2 induces reproducible changes in sequence and abundance of mature microRNAs in the mouse brain.

Vesely C, Tauber S, Sedlazeck FJ, Tajaddod M, von Haeseler A, Jantsch MF - Nucleic Acids Res. (2014)

Bottom Line: For the highly edited miR-3099, we experimentally prove retargeting of the edited miRNA to novel 3' UTRs.We show further that an abundant editing event in miR-497 promotes processing by Drosha of the corresponding pri-miRNA.This indicates that ADAR2 binding but not editing of miRNA precursors may influence their processing.

View Article: PubMed Central - PubMed

Affiliation: Department of Chromosome Biology, Max F. Perutz Laboratories, University of Vienna, Dr. Bohr Gasse 9, A-1030 Vienna, Austria.

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Editing events are enriched in the seed sequence of mature miRNAs. The frequencies of all detected editing events within the mature miRNAs (22 nts average length) are plotted in absolute numbers. The distribution of editing events shows a clear preference for the nucleotides 2–7, all located within the seed region.
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Figure 5: Editing events are enriched in the seed sequence of mature miRNAs. The frequencies of all detected editing events within the mature miRNAs (22 nts average length) are plotted in absolute numbers. The distribution of editing events shows a clear preference for the nucleotides 2–7, all located within the seed region.

Mentions: When examining the positions that showed significant editing events in mature miRNAs, we found that 64% of all editing events occur at position 2–7, namely the seed sequence (Figure 5). Only 46% of all editing events occur in the remaining 16 positions, if an average length of 22 nucleotides is assumed. This enrichment of editing in the seed sequence shows that ADARs have the potential to play an important role in the modulation of miRNA target specificity in the brain.


ADAR2 induces reproducible changes in sequence and abundance of mature microRNAs in the mouse brain.

Vesely C, Tauber S, Sedlazeck FJ, Tajaddod M, von Haeseler A, Jantsch MF - Nucleic Acids Res. (2014)

Editing events are enriched in the seed sequence of mature miRNAs. The frequencies of all detected editing events within the mature miRNAs (22 nts average length) are plotted in absolute numbers. The distribution of editing events shows a clear preference for the nucleotides 2–7, all located within the seed region.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4231736&req=5

Figure 5: Editing events are enriched in the seed sequence of mature miRNAs. The frequencies of all detected editing events within the mature miRNAs (22 nts average length) are plotted in absolute numbers. The distribution of editing events shows a clear preference for the nucleotides 2–7, all located within the seed region.
Mentions: When examining the positions that showed significant editing events in mature miRNAs, we found that 64% of all editing events occur at position 2–7, namely the seed sequence (Figure 5). Only 46% of all editing events occur in the remaining 16 positions, if an average length of 22 nucleotides is assumed. This enrichment of editing in the seed sequence shows that ADARs have the potential to play an important role in the modulation of miRNA target specificity in the brain.

Bottom Line: For the highly edited miR-3099, we experimentally prove retargeting of the edited miRNA to novel 3' UTRs.We show further that an abundant editing event in miR-497 promotes processing by Drosha of the corresponding pri-miRNA.This indicates that ADAR2 binding but not editing of miRNA precursors may influence their processing.

View Article: PubMed Central - PubMed

Affiliation: Department of Chromosome Biology, Max F. Perutz Laboratories, University of Vienna, Dr. Bohr Gasse 9, A-1030 Vienna, Austria.

Show MeSH
Related in: MedlinePlus