ADAR2 induces reproducible changes in sequence and abundance of mature microRNAs in the mouse brain.
Bottom Line: For the highly edited miR-3099, we experimentally prove retargeting of the edited miRNA to novel 3' UTRs.We show further that an abundant editing event in miR-497 promotes processing by Drosha of the corresponding pri-miRNA.This indicates that ADAR2 binding but not editing of miRNA precursors may influence their processing.
Affiliation: Department of Chromosome Biology, Max F. Perutz Laboratories, University of Vienna, Dr. Bohr Gasse 9, A-1030 Vienna, Austria.Show MeSH
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Mentions: When examining the positions that showed significant editing events in mature miRNAs, we found that 64% of all editing events occur at position 2–7, namely the seed sequence (Figure 5). Only 46% of all editing events occur in the remaining 16 positions, if an average length of 22 nucleotides is assumed. This enrichment of editing in the seed sequence shows that ADARs have the potential to play an important role in the modulation of miRNA target specificity in the brain.
Affiliation: Department of Chromosome Biology, Max F. Perutz Laboratories, University of Vienna, Dr. Bohr Gasse 9, A-1030 Vienna, Austria.