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Pogo-like transposases have been repeatedly domesticated into CENP-B-related proteins.

Mateo L, González J - Genome Biol Evol (2014)

Bottom Line: Among the different centromere-associated proteins (CENP) identified, CENP-B has been independently domesticated from a pogo-like transposase twice: Once in mammals and once in fission yeast.Our results showed that CENP-B related genes are not restricted to holocentric insects.Our results highlight the importance of transposable elements as raw material for the recurrent evolution of important cellular functions.

View Article: PubMed Central - PubMed

Affiliation: Institute of Evolutionary Biology (CSIC- Universitat Pompeu Fabra), Barcelona, Spain.

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Phylogenetic distribution of pogo-related transposases and transposase-derived genes in metazoans. JR and CR indicate that the sequences belong to the JR clade and the CR clades, respectively. Filled-boxes depict pogo-related transposases and empty boxes depict transposase-derived genes. Numbers in the nodes show posterior probabilities (black) and bootstrap values (red). Shaded branches correspond to new CR proteins identified in this work and in d'Alençon et al 2011 (table 1) that have been incorporated to the previously published phylogeny (Casola et al 2008). Dotted lines represent branches not drawn to scale. Trees including nonmetazoans pogo-related transposases and transposase-derived genes are depicted in supplementary figures S2 and S3, Supplementary Material online.
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evu153-F3: Phylogenetic distribution of pogo-related transposases and transposase-derived genes in metazoans. JR and CR indicate that the sequences belong to the JR clade and the CR clades, respectively. Filled-boxes depict pogo-related transposases and empty boxes depict transposase-derived genes. Numbers in the nodes show posterior probabilities (black) and bootstrap values (red). Shaded branches correspond to new CR proteins identified in this work and in d'Alençon et al 2011 (table 1) that have been incorporated to the previously published phylogeny (Casola et al 2008). Dotted lines represent branches not drawn to scale. Trees including nonmetazoans pogo-related transposases and transposase-derived genes are depicted in supplementary figures S2 and S3, Supplementary Material online.

Mentions: We constructed a phylogenetic tree to find out where insect CENP-B homologs are located in the previously published phylogeny containing a representative set of pogo transposases and pogo-derived genes (Casola et al. 2008). Phylogenetic trees of the full sequence set containing nonmetazoan transposases and transposase-derived genes can be found in supplementary figures S2 and S3, Supplementary Material online (see Materials and Methods). Our tree recovers the two monophyletic clades in metazoans: CR and Jerky related (JR) (fig. 3). CAG is located in the CR clade, and as expected, its closest transposase is the D. melanogaster pogo. The closest non-Drosophila CAG homolog is Tribolium castaneum TC005011. Most of the other insect CENP-B homolog genes, including the already described S. frugiperda and H. armigera CENP-B homologs, also fell in the CR clade. Insect and mammalian CR proteins form subclades inside the CR clade (fig. 3). Other than between D. melanogaster_CAG and D. ananassae_GF13390 transposase-derived genes, synteny is also conserved among Sfru_72F01, Harmi_94B11_25, and Bombyx_ BGIBMGA013624 suggesting that at least two additional independent exaptations, besides the mammal and fission yeast exaptations reported by Casola et al (2008), have occurred.Fig. 3.—


Pogo-like transposases have been repeatedly domesticated into CENP-B-related proteins.

Mateo L, González J - Genome Biol Evol (2014)

Phylogenetic distribution of pogo-related transposases and transposase-derived genes in metazoans. JR and CR indicate that the sequences belong to the JR clade and the CR clades, respectively. Filled-boxes depict pogo-related transposases and empty boxes depict transposase-derived genes. Numbers in the nodes show posterior probabilities (black) and bootstrap values (red). Shaded branches correspond to new CR proteins identified in this work and in d'Alençon et al 2011 (table 1) that have been incorporated to the previously published phylogeny (Casola et al 2008). Dotted lines represent branches not drawn to scale. Trees including nonmetazoans pogo-related transposases and transposase-derived genes are depicted in supplementary figures S2 and S3, Supplementary Material online.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4231638&req=5

evu153-F3: Phylogenetic distribution of pogo-related transposases and transposase-derived genes in metazoans. JR and CR indicate that the sequences belong to the JR clade and the CR clades, respectively. Filled-boxes depict pogo-related transposases and empty boxes depict transposase-derived genes. Numbers in the nodes show posterior probabilities (black) and bootstrap values (red). Shaded branches correspond to new CR proteins identified in this work and in d'Alençon et al 2011 (table 1) that have been incorporated to the previously published phylogeny (Casola et al 2008). Dotted lines represent branches not drawn to scale. Trees including nonmetazoans pogo-related transposases and transposase-derived genes are depicted in supplementary figures S2 and S3, Supplementary Material online.
Mentions: We constructed a phylogenetic tree to find out where insect CENP-B homologs are located in the previously published phylogeny containing a representative set of pogo transposases and pogo-derived genes (Casola et al. 2008). Phylogenetic trees of the full sequence set containing nonmetazoan transposases and transposase-derived genes can be found in supplementary figures S2 and S3, Supplementary Material online (see Materials and Methods). Our tree recovers the two monophyletic clades in metazoans: CR and Jerky related (JR) (fig. 3). CAG is located in the CR clade, and as expected, its closest transposase is the D. melanogaster pogo. The closest non-Drosophila CAG homolog is Tribolium castaneum TC005011. Most of the other insect CENP-B homolog genes, including the already described S. frugiperda and H. armigera CENP-B homologs, also fell in the CR clade. Insect and mammalian CR proteins form subclades inside the CR clade (fig. 3). Other than between D. melanogaster_CAG and D. ananassae_GF13390 transposase-derived genes, synteny is also conserved among Sfru_72F01, Harmi_94B11_25, and Bombyx_ BGIBMGA013624 suggesting that at least two additional independent exaptations, besides the mammal and fission yeast exaptations reported by Casola et al (2008), have occurred.Fig. 3.—

Bottom Line: Among the different centromere-associated proteins (CENP) identified, CENP-B has been independently domesticated from a pogo-like transposase twice: Once in mammals and once in fission yeast.Our results showed that CENP-B related genes are not restricted to holocentric insects.Our results highlight the importance of transposable elements as raw material for the recurrent evolution of important cellular functions.

View Article: PubMed Central - PubMed

Affiliation: Institute of Evolutionary Biology (CSIC- Universitat Pompeu Fabra), Barcelona, Spain.

Show MeSH