Limits...
Genome evolution and plasticity of Serratia marcescens, an important multidrug-resistant nosocomial pathogen.

Iguchi A, Nagaya Y, Pradel E, Ooka T, Ogura Y, Katsura K, Kurokawa K, Oshima K, Hattori M, Parkhill J, Sebaihia M, Coulthurst SJ, Gotoh N, Thomson NR, Ewbank JJ, Hayashi T - Genome Biol Evol (2014)

Bottom Line: Naturally, it is found in many environmental niches, and is capable of infecting plants and animals.We further show that pSMC1 is most closely related to plasmids circulating in Pseudomonas species.Our data will provide a valuable basis for future studies on S. marcescens and new insights into the genetic mechanisms that underlie the emergence of pathogens highly resistant to multiple antimicrobial agents.

View Article: PubMed Central - PubMed

Affiliation: Interdisciplinary Research Organization, University of Miyazaki, JapanPresent address: Department of Animal and Grassland Sciences, Faculty of Agriculture, University of Miyazaki, Japan.

Show MeSH

Related in: MedlinePlus

Comparison of the SM39 and Db11 genomic loci bearing exopolysaccharide biosynthesis gene clusters. The gene organization of the gene clusters for O antigen biosynthesis and for group 1 CPS biosynthesis is compared between SM39 (untypeable) and Db11 (O28:K7). The O antigen biosynthesis genes of SM39 and Db11 show high level of similarities to those of Klebsiella pneumoniae O8 and those of K. pneumoniae O5, respectively. The Db11 operon also has a high level of similarity to that of Escherichia coli O8 (Iguchi A, Iyoda S, Kikuchi T, Ogura Y, Katsura K, Ohnishi M, Hayashi T and Thomson NR, unpublished data), consistent with the cross-reactivity between Serratia marcescens O28, K. pneumoniae O5, and E. coli O8 antigens previously reported by Aucken and Pitt (1991).
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4231636&req=5

evu160-F4: Comparison of the SM39 and Db11 genomic loci bearing exopolysaccharide biosynthesis gene clusters. The gene organization of the gene clusters for O antigen biosynthesis and for group 1 CPS biosynthesis is compared between SM39 (untypeable) and Db11 (O28:K7). The O antigen biosynthesis genes of SM39 and Db11 show high level of similarities to those of Klebsiella pneumoniae O8 and those of K. pneumoniae O5, respectively. The Db11 operon also has a high level of similarity to that of Escherichia coli O8 (Iguchi A, Iyoda S, Kikuchi T, Ogura Y, Katsura K, Ohnishi M, Hayashi T and Thomson NR, unpublished data), consistent with the cross-reactivity between Serratia marcescens O28, K. pneumoniae O5, and E. coli O8 antigens previously reported by Aucken and Pitt (1991).

Mentions: The gene cluster for O antigen biosynthesis was identified between the S-layer biosynthesis gene cluster and the his operon in both strains (fig. 4). As expected, the gene contents of the loci significantly differ between the two strains; Db11 was serotyped conventionally as O28:K7, whereas SM39 was untypeable. There is also good evidence from the genome sequences that both strains carry a complete gene cluster for group 1 capsule polysaccharide (CPS) biosynthesis, equivalent to the colanic acid biosynthesis genes in E. coli. Differences in gene content in this region suggest, however, that the two strains produce different types of group 1 CPS (fig. 4). This will need to be addressed experimentally in future studies.Fig. 4.—


Genome evolution and plasticity of Serratia marcescens, an important multidrug-resistant nosocomial pathogen.

Iguchi A, Nagaya Y, Pradel E, Ooka T, Ogura Y, Katsura K, Kurokawa K, Oshima K, Hattori M, Parkhill J, Sebaihia M, Coulthurst SJ, Gotoh N, Thomson NR, Ewbank JJ, Hayashi T - Genome Biol Evol (2014)

Comparison of the SM39 and Db11 genomic loci bearing exopolysaccharide biosynthesis gene clusters. The gene organization of the gene clusters for O antigen biosynthesis and for group 1 CPS biosynthesis is compared between SM39 (untypeable) and Db11 (O28:K7). The O antigen biosynthesis genes of SM39 and Db11 show high level of similarities to those of Klebsiella pneumoniae O8 and those of K. pneumoniae O5, respectively. The Db11 operon also has a high level of similarity to that of Escherichia coli O8 (Iguchi A, Iyoda S, Kikuchi T, Ogura Y, Katsura K, Ohnishi M, Hayashi T and Thomson NR, unpublished data), consistent with the cross-reactivity between Serratia marcescens O28, K. pneumoniae O5, and E. coli O8 antigens previously reported by Aucken and Pitt (1991).
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4231636&req=5

evu160-F4: Comparison of the SM39 and Db11 genomic loci bearing exopolysaccharide biosynthesis gene clusters. The gene organization of the gene clusters for O antigen biosynthesis and for group 1 CPS biosynthesis is compared between SM39 (untypeable) and Db11 (O28:K7). The O antigen biosynthesis genes of SM39 and Db11 show high level of similarities to those of Klebsiella pneumoniae O8 and those of K. pneumoniae O5, respectively. The Db11 operon also has a high level of similarity to that of Escherichia coli O8 (Iguchi A, Iyoda S, Kikuchi T, Ogura Y, Katsura K, Ohnishi M, Hayashi T and Thomson NR, unpublished data), consistent with the cross-reactivity between Serratia marcescens O28, K. pneumoniae O5, and E. coli O8 antigens previously reported by Aucken and Pitt (1991).
Mentions: The gene cluster for O antigen biosynthesis was identified between the S-layer biosynthesis gene cluster and the his operon in both strains (fig. 4). As expected, the gene contents of the loci significantly differ between the two strains; Db11 was serotyped conventionally as O28:K7, whereas SM39 was untypeable. There is also good evidence from the genome sequences that both strains carry a complete gene cluster for group 1 capsule polysaccharide (CPS) biosynthesis, equivalent to the colanic acid biosynthesis genes in E. coli. Differences in gene content in this region suggest, however, that the two strains produce different types of group 1 CPS (fig. 4). This will need to be addressed experimentally in future studies.Fig. 4.—

Bottom Line: Naturally, it is found in many environmental niches, and is capable of infecting plants and animals.We further show that pSMC1 is most closely related to plasmids circulating in Pseudomonas species.Our data will provide a valuable basis for future studies on S. marcescens and new insights into the genetic mechanisms that underlie the emergence of pathogens highly resistant to multiple antimicrobial agents.

View Article: PubMed Central - PubMed

Affiliation: Interdisciplinary Research Organization, University of Miyazaki, JapanPresent address: Department of Animal and Grassland Sciences, Faculty of Agriculture, University of Miyazaki, Japan.

Show MeSH
Related in: MedlinePlus