Limits...
The positive correlations of apolipoprotein E with disease activity and related cytokines in systemic lupus erythematosus.

Song LJ, Liu WW, Fan YC, Qiu F, Chen QL, Li XF, Ding F - Diagn Pathol (2013)

Bottom Line: Forty treatment-naïve SLE patients and forty matched healthy controls were studied.All the SLE patients received prednisone 1 mg/kg/day for 28 consecutive days.ApoE sera concentrations positively correlated with SLEDAI, anti-dsDNA antibody and the related cytokines including IL-6, IFN-γ and IL-10, and uncorrelated with the concentration of total cholesterol (TC) and triglyceride (TG) in SLE patients.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Rheumatology, Qilu Hospital, Shandong University, 107# Wenhua Xi Road, Jinan 250012, P,R, China. rheum_666@163.com.

ABSTRACT

Background: The aim of this study is to investigate the expression of apolipoprotein E (apoE) and the relationship between apoE and disease activity of SLE, and the possible effects of glucocorticoid on apoE and other cytokines activities in SLE patients.

Methods: Forty treatment-naïve SLE patients and forty matched healthy controls were studied. All the SLE patients received prednisone 1 mg/kg/day for 28 consecutive days. The sera levels of apoE and related cytokines were evaluated by ELISA. The expression of apoE mRNA in peripheral blood mononuclear cells (PBMCs) was determined by real-time PCR.

Results: Compared with healthy controls, the relative expression levels of ApoE proteins and sera levels were significantly up-regulated in active SLE patients. ApoE sera concentrations positively correlated with SLEDAI, anti-dsDNA antibody and the related cytokines including IL-6, IFN-γ and IL-10, and uncorrelated with the concentration of total cholesterol (TC) and triglyceride (TG) in SLE patients. After 4 weeks prednisone treatment, the relative mRNA expression of apoE and the serum levels of apoE and related cytokines decreased.

Conclusions: ApoE correlated with disease activity and related cytokines in SLE patients. Glucocorticoid can down-regulate the expressions of apoE and related cytokines.

Virtual slide: The virtual slide(s) for this article can be found here:http://www.diagnosticpathology.diagnomx.eu/vs/1646714011077325.

Show MeSH
Spearman rank correlation analysis of apoE with anti-dsDNA antibody andSLEDAI in SLE patients. The sera apoE concentrations were positivelycorrelated with anti-dsDNA antibody (r = 0.64,p < 0.01) and SLEDAI (r = 0.71,p < 0.01) in pre-treatment SLE patients. After prednisonetreatment, the sera apoE concentrations were still positively correlated withanti-dsDNA antibody (r = 0.56, p < 0.01) and SLEDAI(r = 0.65, p < 0.01).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC4231472&req=5

Figure 2: Spearman rank correlation analysis of apoE with anti-dsDNA antibody andSLEDAI in SLE patients. The sera apoE concentrations were positivelycorrelated with anti-dsDNA antibody (r = 0.64,p < 0.01) and SLEDAI (r = 0.71,p < 0.01) in pre-treatment SLE patients. After prednisonetreatment, the sera apoE concentrations were still positively correlated withanti-dsDNA antibody (r = 0.56, p < 0.01) and SLEDAI(r = 0.65, p < 0.01).

Mentions: ApoE levels revealed positive correlation with anti-dsDNA antibody in bothpre-treatment SLE patients (r = 0.64, p < 0.01) andpost-treatment patients (r = 0.56, p < 0.01)(Figure 2). Furthermore, a strong correlation was foundbetween apoE sera levels and SLEDAI in pre-treatment patients (r = 0.71,p < 0.01) and post-treatment patients (r = 0.65,p < 0.01) (Figure 2). The sera apoEconcentrations positively correlated with IL-6 (r = 0.57,p < 0.01), IFN-γ (r = 0.78, p < 0.01)and IL-10 (r = 0.76, p < 0.01) in pre-treatment SLEpatients. After prednisone treatment, the sera apoE concentrations still positivelycorrelated with IL-6 (r = 0.54, p < 0.01), IFN-γ(r = 0.82, p < 0.01) and IL-10 (r = 0.70,p < 0.01) (Figure 3). In healthy controls,the sera apoE concentrations also positively correlated with IL-6(r = 0.87, p < 0.01), IFN-γ (r = 0.68,p < 0.01) and IL-10 (r = 0.72,p < 0.01).


The positive correlations of apolipoprotein E with disease activity and related cytokines in systemic lupus erythematosus.

Song LJ, Liu WW, Fan YC, Qiu F, Chen QL, Li XF, Ding F - Diagn Pathol (2013)

Spearman rank correlation analysis of apoE with anti-dsDNA antibody andSLEDAI in SLE patients. The sera apoE concentrations were positivelycorrelated with anti-dsDNA antibody (r = 0.64,p < 0.01) and SLEDAI (r = 0.71,p < 0.01) in pre-treatment SLE patients. After prednisonetreatment, the sera apoE concentrations were still positively correlated withanti-dsDNA antibody (r = 0.56, p < 0.01) and SLEDAI(r = 0.65, p < 0.01).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4231472&req=5

Figure 2: Spearman rank correlation analysis of apoE with anti-dsDNA antibody andSLEDAI in SLE patients. The sera apoE concentrations were positivelycorrelated with anti-dsDNA antibody (r = 0.64,p < 0.01) and SLEDAI (r = 0.71,p < 0.01) in pre-treatment SLE patients. After prednisonetreatment, the sera apoE concentrations were still positively correlated withanti-dsDNA antibody (r = 0.56, p < 0.01) and SLEDAI(r = 0.65, p < 0.01).
Mentions: ApoE levels revealed positive correlation with anti-dsDNA antibody in bothpre-treatment SLE patients (r = 0.64, p < 0.01) andpost-treatment patients (r = 0.56, p < 0.01)(Figure 2). Furthermore, a strong correlation was foundbetween apoE sera levels and SLEDAI in pre-treatment patients (r = 0.71,p < 0.01) and post-treatment patients (r = 0.65,p < 0.01) (Figure 2). The sera apoEconcentrations positively correlated with IL-6 (r = 0.57,p < 0.01), IFN-γ (r = 0.78, p < 0.01)and IL-10 (r = 0.76, p < 0.01) in pre-treatment SLEpatients. After prednisone treatment, the sera apoE concentrations still positivelycorrelated with IL-6 (r = 0.54, p < 0.01), IFN-γ(r = 0.82, p < 0.01) and IL-10 (r = 0.70,p < 0.01) (Figure 3). In healthy controls,the sera apoE concentrations also positively correlated with IL-6(r = 0.87, p < 0.01), IFN-γ (r = 0.68,p < 0.01) and IL-10 (r = 0.72,p < 0.01).

Bottom Line: Forty treatment-naïve SLE patients and forty matched healthy controls were studied.All the SLE patients received prednisone 1 mg/kg/day for 28 consecutive days.ApoE sera concentrations positively correlated with SLEDAI, anti-dsDNA antibody and the related cytokines including IL-6, IFN-γ and IL-10, and uncorrelated with the concentration of total cholesterol (TC) and triglyceride (TG) in SLE patients.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Rheumatology, Qilu Hospital, Shandong University, 107# Wenhua Xi Road, Jinan 250012, P,R, China. rheum_666@163.com.

ABSTRACT

Background: The aim of this study is to investigate the expression of apolipoprotein E (apoE) and the relationship between apoE and disease activity of SLE, and the possible effects of glucocorticoid on apoE and other cytokines activities in SLE patients.

Methods: Forty treatment-naïve SLE patients and forty matched healthy controls were studied. All the SLE patients received prednisone 1 mg/kg/day for 28 consecutive days. The sera levels of apoE and related cytokines were evaluated by ELISA. The expression of apoE mRNA in peripheral blood mononuclear cells (PBMCs) was determined by real-time PCR.

Results: Compared with healthy controls, the relative expression levels of ApoE proteins and sera levels were significantly up-regulated in active SLE patients. ApoE sera concentrations positively correlated with SLEDAI, anti-dsDNA antibody and the related cytokines including IL-6, IFN-γ and IL-10, and uncorrelated with the concentration of total cholesterol (TC) and triglyceride (TG) in SLE patients. After 4 weeks prednisone treatment, the relative mRNA expression of apoE and the serum levels of apoE and related cytokines decreased.

Conclusions: ApoE correlated with disease activity and related cytokines in SLE patients. Glucocorticoid can down-regulate the expressions of apoE and related cytokines.

Virtual slide: The virtual slide(s) for this article can be found here:http://www.diagnosticpathology.diagnomx.eu/vs/1646714011077325.

Show MeSH