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Effect of methotrexate conjugated PAMAM dendrimers on the viability of MES-SA uterine cancer cells.

Khatri S, Das NG, Das SK - J Pharm Bioallied Sci (2014)

Bottom Line: The amide-bonded PAMAM dendrimer-MTX conjugates were prepared by conjugation between the amine-terminated G5 dendrimer and the carboxylic groups of the MTX using a dicyclohexylcarbodiimide coupling reaction.The UV and (1)H NMR study confirmed the formation of covalent bonds between the drug and the dendrimer.The cell viability study indicated that the nanoconjugates had significantly improved cell killing compared to the free MTX.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutical Sciences, Butler University, Indianapolis, IN 46208, USA.

ABSTRACT
The aim of this work was to synthesize methotrexate (MTX)-polyamidoamine (PAMAM) dendritic nanoconjugates and to study their effect on cell viability in uterine sarcoma cells. The amide-bonded PAMAM dendrimer-MTX conjugates were prepared by conjugation between the amine-terminated G5 dendrimer and the carboxylic groups of the MTX using a dicyclohexylcarbodiimide coupling reaction. The formation of conjugates was evaluated by ultraviolet (UV) and (1)H nuclear magnetic resonance ((1)H NMR) spectroscopy studies. The cell survival of MES-SA cells, a uterine sarcoma cell line, was evaluated in the presence of the dendrimer-MTX nanoconjugate, using appropriate controls. The UV and (1)H NMR study confirmed the formation of covalent bonds between the drug and the dendrimer. The cell viability study indicated that the nanoconjugates had significantly improved cell killing compared to the free MTX.

No MeSH data available.


Related in: MedlinePlus

MES-SA growth curve
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Related In: Results  -  Collection

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Figure 3: MES-SA growth curve

Mentions: Knowledge of the time frames required for the doubling of cells and reproducible results regarding their healthy proliferation ensures that the results obtained from the cell survival studies (MTT assay) are reliable. The short-term growth rate of the MES-SA cells was determined to be linear with a correlation coefficient of >0.90 (R2 = 0.9082). The growth curve is shown in Figure 3. The results obtained validated the treatment schedule, i.e. the test formulations were being applied to the MES-SA cells in appropriate intervals.


Effect of methotrexate conjugated PAMAM dendrimers on the viability of MES-SA uterine cancer cells.

Khatri S, Das NG, Das SK - J Pharm Bioallied Sci (2014)

MES-SA growth curve
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4231390&req=5

Figure 3: MES-SA growth curve
Mentions: Knowledge of the time frames required for the doubling of cells and reproducible results regarding their healthy proliferation ensures that the results obtained from the cell survival studies (MTT assay) are reliable. The short-term growth rate of the MES-SA cells was determined to be linear with a correlation coefficient of >0.90 (R2 = 0.9082). The growth curve is shown in Figure 3. The results obtained validated the treatment schedule, i.e. the test formulations were being applied to the MES-SA cells in appropriate intervals.

Bottom Line: The amide-bonded PAMAM dendrimer-MTX conjugates were prepared by conjugation between the amine-terminated G5 dendrimer and the carboxylic groups of the MTX using a dicyclohexylcarbodiimide coupling reaction.The UV and (1)H NMR study confirmed the formation of covalent bonds between the drug and the dendrimer.The cell viability study indicated that the nanoconjugates had significantly improved cell killing compared to the free MTX.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutical Sciences, Butler University, Indianapolis, IN 46208, USA.

ABSTRACT
The aim of this work was to synthesize methotrexate (MTX)-polyamidoamine (PAMAM) dendritic nanoconjugates and to study their effect on cell viability in uterine sarcoma cells. The amide-bonded PAMAM dendrimer-MTX conjugates were prepared by conjugation between the amine-terminated G5 dendrimer and the carboxylic groups of the MTX using a dicyclohexylcarbodiimide coupling reaction. The formation of conjugates was evaluated by ultraviolet (UV) and (1)H nuclear magnetic resonance ((1)H NMR) spectroscopy studies. The cell survival of MES-SA cells, a uterine sarcoma cell line, was evaluated in the presence of the dendrimer-MTX nanoconjugate, using appropriate controls. The UV and (1)H NMR study confirmed the formation of covalent bonds between the drug and the dendrimer. The cell viability study indicated that the nanoconjugates had significantly improved cell killing compared to the free MTX.

No MeSH data available.


Related in: MedlinePlus