Toll-like receptor expression in crypt epithelial cells, putative stem cells and intestinal myofibroblasts isolated from controls and patients with inflammatory bowel disease.
Bottom Line: In conclusion, enhanced TLR-2 and TLR-4 expression by crypt epithelial cells in active inflammatory bowel disease likely reflects greater ability to respond to microbial products.Results from our studies using mucosal samples from patients with distal ulcerative colitis suggest that the enhanced expression of these TLRs could be constitutive.TLR-2, TLR-4 and TLR-5 expression by stem cells imply ability to respond to distinct bacterial products.
Affiliation: Institute of Infection, Immunity and Inflammation, University of Nottingham, UK; Nottingham Digestive Diseases Centre, University of Nottingham, UK.Show MeSH
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Mentions: Using conventional RT–PCR, myofibroblasts isolated from normal control and active IBD mucosal samples showed PCR products specific for TLR-2 and TLR-4 (not shown). TLR-2 and TLR-4 protein expression was confirmed by Western blot analysis, but the level of expression was much lower than that for isolated crypt epithelial cells (Fig. 8).
Affiliation: Institute of Infection, Immunity and Inflammation, University of Nottingham, UK; Nottingham Digestive Diseases Centre, University of Nottingham, UK.