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Toll-like receptor expression in crypt epithelial cells, putative stem cells and intestinal myofibroblasts isolated from controls and patients with inflammatory bowel disease.

Brown M, Hughes KR, Moossavi S, Robins A, Mahida YR - Clin. Exp. Immunol. (2014)

Bottom Line: In conclusion, enhanced TLR-2 and TLR-4 expression by crypt epithelial cells in active inflammatory bowel disease likely reflects greater ability to respond to microbial products.Results from our studies using mucosal samples from patients with distal ulcerative colitis suggest that the enhanced expression of these TLRs could be constitutive.TLR-2, TLR-4 and TLR-5 expression by stem cells imply ability to respond to distinct bacterial products.

View Article: PubMed Central - PubMed

Affiliation: Institute of Infection, Immunity and Inflammation, University of Nottingham, UK; Nottingham Digestive Diseases Centre, University of Nottingham, UK.

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Relative quantitative expression of Toll-like receptor (TLR)-4 (a) and TLR-2 (b) mRNA transcripts in isolated and disaggregated ileal crypt epithelial cells obtained from mucosal samples affected by active Crohn’s disease (n = 7) and histologically normal control ileal tissue (n = 7). Extracted RNA was used for real-time reverse transcription–polymerase chain reaction (RT–PCR) and data for Crohn’s ileal crypt cells are presented as ‘fold change’ in expression of transcripts compared to mean expression in cells from the control group. Each data point represents mean mRNA expression of three samples per patient and the horizontal bars represent median expression.
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fig02: Relative quantitative expression of Toll-like receptor (TLR)-4 (a) and TLR-2 (b) mRNA transcripts in isolated and disaggregated ileal crypt epithelial cells obtained from mucosal samples affected by active Crohn’s disease (n = 7) and histologically normal control ileal tissue (n = 7). Extracted RNA was used for real-time reverse transcription–polymerase chain reaction (RT–PCR) and data for Crohn’s ileal crypt cells are presented as ‘fold change’ in expression of transcripts compared to mean expression in cells from the control group. Each data point represents mean mRNA expression of three samples per patient and the horizontal bars represent median expression.

Mentions: There was significantly enhanced expression of TLR-4 transcripts in crypt cells isolated from inflamed ileal Crohn’s disease mucosal samples, when compared to cells obtained from normal control ileal tissue [fold increase: 1·84 (1·39–17·69), P = 0·030; Fig. 2a]. Although some Crohn’s ileal crypt cell samples showed high levels of TLR-2 mRNA expression, the difference between the two groups was not statistically significant [fold increase for Crohn’s group: 1·72 (0·23–3·89); Fig. 2b and Supporting information, Table S2].


Toll-like receptor expression in crypt epithelial cells, putative stem cells and intestinal myofibroblasts isolated from controls and patients with inflammatory bowel disease.

Brown M, Hughes KR, Moossavi S, Robins A, Mahida YR - Clin. Exp. Immunol. (2014)

Relative quantitative expression of Toll-like receptor (TLR)-4 (a) and TLR-2 (b) mRNA transcripts in isolated and disaggregated ileal crypt epithelial cells obtained from mucosal samples affected by active Crohn’s disease (n = 7) and histologically normal control ileal tissue (n = 7). Extracted RNA was used for real-time reverse transcription–polymerase chain reaction (RT–PCR) and data for Crohn’s ileal crypt cells are presented as ‘fold change’ in expression of transcripts compared to mean expression in cells from the control group. Each data point represents mean mRNA expression of three samples per patient and the horizontal bars represent median expression.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4231243&req=5

fig02: Relative quantitative expression of Toll-like receptor (TLR)-4 (a) and TLR-2 (b) mRNA transcripts in isolated and disaggregated ileal crypt epithelial cells obtained from mucosal samples affected by active Crohn’s disease (n = 7) and histologically normal control ileal tissue (n = 7). Extracted RNA was used for real-time reverse transcription–polymerase chain reaction (RT–PCR) and data for Crohn’s ileal crypt cells are presented as ‘fold change’ in expression of transcripts compared to mean expression in cells from the control group. Each data point represents mean mRNA expression of three samples per patient and the horizontal bars represent median expression.
Mentions: There was significantly enhanced expression of TLR-4 transcripts in crypt cells isolated from inflamed ileal Crohn’s disease mucosal samples, when compared to cells obtained from normal control ileal tissue [fold increase: 1·84 (1·39–17·69), P = 0·030; Fig. 2a]. Although some Crohn’s ileal crypt cell samples showed high levels of TLR-2 mRNA expression, the difference between the two groups was not statistically significant [fold increase for Crohn’s group: 1·72 (0·23–3·89); Fig. 2b and Supporting information, Table S2].

Bottom Line: In conclusion, enhanced TLR-2 and TLR-4 expression by crypt epithelial cells in active inflammatory bowel disease likely reflects greater ability to respond to microbial products.Results from our studies using mucosal samples from patients with distal ulcerative colitis suggest that the enhanced expression of these TLRs could be constitutive.TLR-2, TLR-4 and TLR-5 expression by stem cells imply ability to respond to distinct bacterial products.

View Article: PubMed Central - PubMed

Affiliation: Institute of Infection, Immunity and Inflammation, University of Nottingham, UK; Nottingham Digestive Diseases Centre, University of Nottingham, UK.

Show MeSH
Related in: MedlinePlus