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Phenotypic heterogeneity is a selected trait in natural yeast populations subject to environmental stress.

Holland SL, Reader T, Dyer PS, Avery SV - Environ. Microbiol. (2013)

Bottom Line: We found that cell-to-cell heterogeneity (in resistance to the appropriate principal pollutant) was prevalent in the wild yeast isolates.Indeed, growth assays indicated that isolates with high heterogeneities had a significant competitive advantage during stress.The results showed that environmental stress selects for wild microorganisms with high levels of phenotypic heterogeneity.

View Article: PubMed Central - PubMed

Affiliation: School of Life Sciences, University of Nottingham, University Park, Nottingham, NG7 2RD, UK.

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Related in: MedlinePlus

Heterogeneity of C. sake isolates near a Pb-polluted mine site. Independent isolates of C. sake obtained from control (□) or Pb-polluted (▪) locations at Site 2 were spread plated to MYP agar supplemented with different Pb(NO3)2 concentrations. Colonies were enumerated after 14 days, and dose-response curves were constructed. Heterogeneity (A) and IC50 (B) determinations were as described in the legend to Fig. 2.
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fig03: Heterogeneity of C. sake isolates near a Pb-polluted mine site. Independent isolates of C. sake obtained from control (□) or Pb-polluted (▪) locations at Site 2 were spread plated to MYP agar supplemented with different Pb(NO3)2 concentrations. Colonies were enumerated after 14 days, and dose-response curves were constructed. Heterogeneity (A) and IC50 (B) determinations were as described in the legend to Fig. 2.

Mentions: Considering Site 2, a total of 25 independent isolates of C. sake were collected from the control and Pb-polluted locations at this site. Similar to the trend seen at Site 1, the five least heterogeneous isolates from the control location were all less heterogeneous than any of the isolates from the polluted location (Fig. 3A). Accordingly, the mean heterogeneity was ∼ 42% lower for the control isolates, although the difference was not significant in this case (P = 0.0695). In contrast to heterogeneity, the mean IC50 across isolates from the polluted location was only marginally (< 2%) greater than that across isolates from the control location. The difference was not significant (P = 0.276) (Fig. 3B).


Phenotypic heterogeneity is a selected trait in natural yeast populations subject to environmental stress.

Holland SL, Reader T, Dyer PS, Avery SV - Environ. Microbiol. (2013)

Heterogeneity of C. sake isolates near a Pb-polluted mine site. Independent isolates of C. sake obtained from control (□) or Pb-polluted (▪) locations at Site 2 were spread plated to MYP agar supplemented with different Pb(NO3)2 concentrations. Colonies were enumerated after 14 days, and dose-response curves were constructed. Heterogeneity (A) and IC50 (B) determinations were as described in the legend to Fig. 2.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4231229&req=5

fig03: Heterogeneity of C. sake isolates near a Pb-polluted mine site. Independent isolates of C. sake obtained from control (□) or Pb-polluted (▪) locations at Site 2 were spread plated to MYP agar supplemented with different Pb(NO3)2 concentrations. Colonies were enumerated after 14 days, and dose-response curves were constructed. Heterogeneity (A) and IC50 (B) determinations were as described in the legend to Fig. 2.
Mentions: Considering Site 2, a total of 25 independent isolates of C. sake were collected from the control and Pb-polluted locations at this site. Similar to the trend seen at Site 1, the five least heterogeneous isolates from the control location were all less heterogeneous than any of the isolates from the polluted location (Fig. 3A). Accordingly, the mean heterogeneity was ∼ 42% lower for the control isolates, although the difference was not significant in this case (P = 0.0695). In contrast to heterogeneity, the mean IC50 across isolates from the polluted location was only marginally (< 2%) greater than that across isolates from the control location. The difference was not significant (P = 0.276) (Fig. 3B).

Bottom Line: We found that cell-to-cell heterogeneity (in resistance to the appropriate principal pollutant) was prevalent in the wild yeast isolates.Indeed, growth assays indicated that isolates with high heterogeneities had a significant competitive advantage during stress.The results showed that environmental stress selects for wild microorganisms with high levels of phenotypic heterogeneity.

View Article: PubMed Central - PubMed

Affiliation: School of Life Sciences, University of Nottingham, University Park, Nottingham, NG7 2RD, UK.

Show MeSH
Related in: MedlinePlus