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Afadin requirement for cytokine expressions in keratinocytes during chemically induced inflammation in mice.

Yoshida T, Iwata T, Takai Y, Birchmeier W, Yamato M, Okano T - Genes Cells (2014)

Bottom Line: Nectin signaling induces formation of cell-cell junctions and is required for the development of epithelial tissues, including skin.Immunohistochemical and quantitative real-time PCR analyses showed that the expression levels of cytokines including Cxcl2, Il-1β and Tnf-α were reduced in CKO keratinocytes compared with control keratinocytes during TPA-induced inflammation.These results suggested a remarkable function of afadin, which was able to enhance cytokine expression through Rap1 activation in keratinocytes during inflammation.

View Article: PubMed Central - PubMed

Affiliation: Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, 8-1 Kawada-cho Shinjuku-ku, Tokyo, 162-8666, Japan.

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Related in: MedlinePlus

Effect of 12-O-tetradecanoylphorbol 13-acetate (TPA) treatment on cultured keratinocytes. (A) The expression levels of the genes shown at the top of each graph in keratinocytes treated with TPA for 2 h were analyzed by real-time RT-PCR. The bars and lines represent the means and standard deviations in primary keratinocytes from seven mice (*P < 0.01). (B) GTP-Rap1 pull-down assay from keratinocyte protein extracts with or without TPA treatment. The picture is the representative of three independent experiments.
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fig08: Effect of 12-O-tetradecanoylphorbol 13-acetate (TPA) treatment on cultured keratinocytes. (A) The expression levels of the genes shown at the top of each graph in keratinocytes treated with TPA for 2 h were analyzed by real-time RT-PCR. The bars and lines represent the means and standard deviations in primary keratinocytes from seven mice (*P < 0.01). (B) GTP-Rap1 pull-down assay from keratinocyte protein extracts with or without TPA treatment. The picture is the representative of three independent experiments.

Mentions: Then, the effect of afadin deficiency on cytokine expression was investigated in keratinocytes in vitro. Keratinocytes were harvested from neonatal mouse back skin and treated with TPA. The expression of Cxcl2,IL-1β and Tnf-α was induced by TPA treatment, and the expression levels of these genes were lower in CKO keratinocytes than in the control keratinocytes (Fig.8A). Although the amount of the GTP-bound active form of Rap1, an afadin-interacting protein, was increased by TPA treatment, only a slightly increase was found in CKO keratinocytes (Fig.8B).


Afadin requirement for cytokine expressions in keratinocytes during chemically induced inflammation in mice.

Yoshida T, Iwata T, Takai Y, Birchmeier W, Yamato M, Okano T - Genes Cells (2014)

Effect of 12-O-tetradecanoylphorbol 13-acetate (TPA) treatment on cultured keratinocytes. (A) The expression levels of the genes shown at the top of each graph in keratinocytes treated with TPA for 2 h were analyzed by real-time RT-PCR. The bars and lines represent the means and standard deviations in primary keratinocytes from seven mice (*P < 0.01). (B) GTP-Rap1 pull-down assay from keratinocyte protein extracts with or without TPA treatment. The picture is the representative of three independent experiments.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4231224&req=5

fig08: Effect of 12-O-tetradecanoylphorbol 13-acetate (TPA) treatment on cultured keratinocytes. (A) The expression levels of the genes shown at the top of each graph in keratinocytes treated with TPA for 2 h were analyzed by real-time RT-PCR. The bars and lines represent the means and standard deviations in primary keratinocytes from seven mice (*P < 0.01). (B) GTP-Rap1 pull-down assay from keratinocyte protein extracts with or without TPA treatment. The picture is the representative of three independent experiments.
Mentions: Then, the effect of afadin deficiency on cytokine expression was investigated in keratinocytes in vitro. Keratinocytes were harvested from neonatal mouse back skin and treated with TPA. The expression of Cxcl2,IL-1β and Tnf-α was induced by TPA treatment, and the expression levels of these genes were lower in CKO keratinocytes than in the control keratinocytes (Fig.8A). Although the amount of the GTP-bound active form of Rap1, an afadin-interacting protein, was increased by TPA treatment, only a slightly increase was found in CKO keratinocytes (Fig.8B).

Bottom Line: Nectin signaling induces formation of cell-cell junctions and is required for the development of epithelial tissues, including skin.Immunohistochemical and quantitative real-time PCR analyses showed that the expression levels of cytokines including Cxcl2, Il-1β and Tnf-α were reduced in CKO keratinocytes compared with control keratinocytes during TPA-induced inflammation.These results suggested a remarkable function of afadin, which was able to enhance cytokine expression through Rap1 activation in keratinocytes during inflammation.

View Article: PubMed Central - PubMed

Affiliation: Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, 8-1 Kawada-cho Shinjuku-ku, Tokyo, 162-8666, Japan.

Show MeSH
Related in: MedlinePlus