Afadin requirement for cytokine expressions in keratinocytes during chemically induced inflammation in mice.
Bottom Line: Nectin signaling induces formation of cell-cell junctions and is required for the development of epithelial tissues, including skin.Immunohistochemical and quantitative real-time PCR analyses showed that the expression levels of cytokines including Cxcl2, Il-1β and Tnf-α were reduced in CKO keratinocytes compared with control keratinocytes during TPA-induced inflammation.These results suggested a remarkable function of afadin, which was able to enhance cytokine expression through Rap1 activation in keratinocytes during inflammation.
Affiliation: Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, 8-1 Kawada-cho Shinjuku-ku, Tokyo, 162-8666, Japan.Show MeSH
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Mentions: Then, the effect of afadin deficiency on cytokine expression was investigated in keratinocytes in vitro. Keratinocytes were harvested from neonatal mouse back skin and treated with TPA. The expression of Cxcl2,IL-1β and Tnf-α was induced by TPA treatment, and the expression levels of these genes were lower in CKO keratinocytes than in the control keratinocytes (Fig.8A). Although the amount of the GTP-bound active form of Rap1, an afadin-interacting protein, was increased by TPA treatment, only a slightly increase was found in CKO keratinocytes (Fig.8B).
Affiliation: Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, 8-1 Kawada-cho Shinjuku-ku, Tokyo, 162-8666, Japan.