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Afadin requirement for cytokine expressions in keratinocytes during chemically induced inflammation in mice.

Yoshida T, Iwata T, Takai Y, Birchmeier W, Yamato M, Okano T - Genes Cells (2014)

Bottom Line: Nectin signaling induces formation of cell-cell junctions and is required for the development of epithelial tissues, including skin.Immunohistochemical and quantitative real-time PCR analyses showed that the expression levels of cytokines including Cxcl2, Il-1β and Tnf-α were reduced in CKO keratinocytes compared with control keratinocytes during TPA-induced inflammation.These results suggested a remarkable function of afadin, which was able to enhance cytokine expression through Rap1 activation in keratinocytes during inflammation.

View Article: PubMed Central - PubMed

Affiliation: Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, 8-1 Kawada-cho Shinjuku-ku, Tokyo, 162-8666, Japan.

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Related in: MedlinePlus

Gene expressions in 12-O-tetradecanoylphorbol 13-acetate (TPA)-treated skin specimens from control and afadin conditional knockout (CKO). Expression levels of the indicated genes shown at the top of each graph in skin treated with TPA for 3 days were analyzed by real-time RT-PCR. The bars and lines represent the means and standard deviations of seven mouse skin samples (*P < 0.01).
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fig05: Gene expressions in 12-O-tetradecanoylphorbol 13-acetate (TPA)-treated skin specimens from control and afadin conditional knockout (CKO). Expression levels of the indicated genes shown at the top of each graph in skin treated with TPA for 3 days were analyzed by real-time RT-PCR. The bars and lines represent the means and standard deviations of seven mouse skin samples (*P < 0.01).

Mentions: RNA was extracted from skin treated with TPA for 3 days, and the expression levels of inflammatory cytokines including Cxcl1,Cxcl2,IL-1β, S100a8,Tgf-β and Tnf-α were investigated using real-time RT-PCR. Among these genes, the expression levels of Cxcl2,IL-1β and Tnf-α were reduced in CKO skin compared with the levels in the control skin (Fig.5). Immunohistochemical analysis using skin treated with TPA for 3 days confirmed that Cxcl2, Il-1β and Tnf-α were expressed in keratinocytes and that the expression levels were reduced in CKO skin compared with control skin (Fig.6). The induction of Cxcl2, Il-1β and Tnf-α was already observed 2 h after the first application of TPA, and expression of these transcripts was reduced in CKO skin compared with control skin (Fig.7).


Afadin requirement for cytokine expressions in keratinocytes during chemically induced inflammation in mice.

Yoshida T, Iwata T, Takai Y, Birchmeier W, Yamato M, Okano T - Genes Cells (2014)

Gene expressions in 12-O-tetradecanoylphorbol 13-acetate (TPA)-treated skin specimens from control and afadin conditional knockout (CKO). Expression levels of the indicated genes shown at the top of each graph in skin treated with TPA for 3 days were analyzed by real-time RT-PCR. The bars and lines represent the means and standard deviations of seven mouse skin samples (*P < 0.01).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4231224&req=5

fig05: Gene expressions in 12-O-tetradecanoylphorbol 13-acetate (TPA)-treated skin specimens from control and afadin conditional knockout (CKO). Expression levels of the indicated genes shown at the top of each graph in skin treated with TPA for 3 days were analyzed by real-time RT-PCR. The bars and lines represent the means and standard deviations of seven mouse skin samples (*P < 0.01).
Mentions: RNA was extracted from skin treated with TPA for 3 days, and the expression levels of inflammatory cytokines including Cxcl1,Cxcl2,IL-1β, S100a8,Tgf-β and Tnf-α were investigated using real-time RT-PCR. Among these genes, the expression levels of Cxcl2,IL-1β and Tnf-α were reduced in CKO skin compared with the levels in the control skin (Fig.5). Immunohistochemical analysis using skin treated with TPA for 3 days confirmed that Cxcl2, Il-1β and Tnf-α were expressed in keratinocytes and that the expression levels were reduced in CKO skin compared with control skin (Fig.6). The induction of Cxcl2, Il-1β and Tnf-α was already observed 2 h after the first application of TPA, and expression of these transcripts was reduced in CKO skin compared with control skin (Fig.7).

Bottom Line: Nectin signaling induces formation of cell-cell junctions and is required for the development of epithelial tissues, including skin.Immunohistochemical and quantitative real-time PCR analyses showed that the expression levels of cytokines including Cxcl2, Il-1β and Tnf-α were reduced in CKO keratinocytes compared with control keratinocytes during TPA-induced inflammation.These results suggested a remarkable function of afadin, which was able to enhance cytokine expression through Rap1 activation in keratinocytes during inflammation.

View Article: PubMed Central - PubMed

Affiliation: Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, 8-1 Kawada-cho Shinjuku-ku, Tokyo, 162-8666, Japan.

Show MeSH
Related in: MedlinePlus