Afadin requirement for cytokine expressions in keratinocytes during chemically induced inflammation in mice.
Bottom Line: Nectin signaling induces formation of cell-cell junctions and is required for the development of epithelial tissues, including skin.Immunohistochemical and quantitative real-time PCR analyses showed that the expression levels of cytokines including Cxcl2, Il-1β and Tnf-α were reduced in CKO keratinocytes compared with control keratinocytes during TPA-induced inflammation.These results suggested a remarkable function of afadin, which was able to enhance cytokine expression through Rap1 activation in keratinocytes during inflammation.
Affiliation: Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, 8-1 Kawada-cho Shinjuku-ku, Tokyo, 162-8666, Japan.Show MeSH
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Mentions: RNA was extracted from skin treated with TPA for 3 days, and the expression levels of inflammatory cytokines including Cxcl1,Cxcl2,IL-1β, S100a8,Tgf-β and Tnf-α were investigated using real-time RT-PCR. Among these genes, the expression levels of Cxcl2,IL-1β and Tnf-α were reduced in CKO skin compared with the levels in the control skin (Fig.5). Immunohistochemical analysis using skin treated with TPA for 3 days confirmed that Cxcl2, Il-1β and Tnf-α were expressed in keratinocytes and that the expression levels were reduced in CKO skin compared with control skin (Fig.6). The induction of Cxcl2, Il-1β and Tnf-α was already observed 2 h after the first application of TPA, and expression of these transcripts was reduced in CKO skin compared with control skin (Fig.7).
Affiliation: Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, 8-1 Kawada-cho Shinjuku-ku, Tokyo, 162-8666, Japan.