Afadin requirement for cytokine expressions in keratinocytes during chemically induced inflammation in mice.
Bottom Line: Nectin signaling induces formation of cell-cell junctions and is required for the development of epithelial tissues, including skin.Immunohistochemical and quantitative real-time PCR analyses showed that the expression levels of cytokines including Cxcl2, Il-1β and Tnf-α were reduced in CKO keratinocytes compared with control keratinocytes during TPA-induced inflammation.These results suggested a remarkable function of afadin, which was able to enhance cytokine expression through Rap1 activation in keratinocytes during inflammation.
Affiliation: Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, 8-1 Kawada-cho Shinjuku-ku, Tokyo, 162-8666, Japan.Show MeSH
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Mentions: This study crossed keratin 14 (K14)-derived Cre-expressing mice, which expressed Cre in the epithelium from embryonic day 15, with afadin floxed mice, and created K14 Cre afadin flox/flox (CKO) mice. In these mice, the expression of normal afadin mRNA in tail skin and expression of afadin protein in back skin were severely reduced (Fig.1A, B). The CKO mice were viable, grew normally, and were fertile. The development of epithelial tissues, including skin and salivary glands, occurred normally (Fig.2A, B, E, F). The teeth of CKO mice showed mild hypomorpy (Fig. S1 in Supporting Information).
Affiliation: Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, 8-1 Kawada-cho Shinjuku-ku, Tokyo, 162-8666, Japan.