Limits...
Construction of microRNA and transcription factor regulatory network based on gene expression data in cardiomyopathy.

Wang L, Hu J, Xing H, Sun M, Wang J, Jian Q, Yang H - Eur. J. Med. Res. (2014)

Bottom Line: TFs and miRNAs that DEGs significantly enriched were identified and a double-factor regulatory network was constructed.The DEGs were enriched for various pathways, with glucocorticoid receptor signaling as the most significant.A subnetwork under the regulation of MEF2C and SRF was also constructed to illustrate their regulatory effects on cardiac functions.

View Article: PubMed Central - PubMed

Affiliation: Department of Cardiology, Xi'an Children's Hospital, 69 Xi Ju Rd, Lianhu District, Xi'an, 710003, China. xawanglei@163.com.

ABSTRACT

Background: Cardiomyopathy is a progressive myocardial disorder. Here, we attempted to reveal the possible mechanism of cardiomyopathy at the transcription level with the roles of microRNAs (miRNAs) and transcription factors (TFs) taken into account.

Method: We firstly identified differentially expressed genes (DEGs) between cardiomyopathy patients and controls with data from the gene expression omnibus (GEO) database. DEGs were associated with the canonical pathways, molecular and cellular functions, physiological system development and function in the Ingenuity Knowledge Base by using the Ingenuity Pathway Analysis (IPA) software. TFs and miRNAs that DEGs significantly enriched were identified and a double-factor regulatory network was constructed.

Results: A total of 1,680 DEGs were identified. The DEGs were enriched for various pathways, with glucocorticoid receptor signaling as the most significant. A double-factor regulatory network was constructed, including seven TFs and two miRNAs. A subnetwork under the regulation of MEF2C and SRF was also constructed to illustrate their regulatory effects on cardiac functions.

Conclusion: Our results may provide new understanding of cardiomyopathy and may facilitate further therapeutic studies.

Show MeSH

Related in: MedlinePlus

The network under the regulation ofSRFandMEF2C. Up-regulated genes are shown in red and down-regulated genes are shown in green. The related cardiac functions are indicated in long ellipses.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC4231188&req=5

Fig3: The network under the regulation ofSRFandMEF2C. Up-regulated genes are shown in red and down-regulated genes are shown in green. The related cardiac functions are indicated in long ellipses.

Mentions: The results of miRNA and TF enrichment analysis are listed in Table 2. A network was constructed to illustrate the regulatory relationships (Figure 2). Considering the important role of SRF and MEF2C in the progression of cardiomyopathy, a subnetwork under their regulation was constructed and the biological functions they may affect were also indicated (Figure 3).Table 2


Construction of microRNA and transcription factor regulatory network based on gene expression data in cardiomyopathy.

Wang L, Hu J, Xing H, Sun M, Wang J, Jian Q, Yang H - Eur. J. Med. Res. (2014)

The network under the regulation ofSRFandMEF2C. Up-regulated genes are shown in red and down-regulated genes are shown in green. The related cardiac functions are indicated in long ellipses.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4231188&req=5

Fig3: The network under the regulation ofSRFandMEF2C. Up-regulated genes are shown in red and down-regulated genes are shown in green. The related cardiac functions are indicated in long ellipses.
Mentions: The results of miRNA and TF enrichment analysis are listed in Table 2. A network was constructed to illustrate the regulatory relationships (Figure 2). Considering the important role of SRF and MEF2C in the progression of cardiomyopathy, a subnetwork under their regulation was constructed and the biological functions they may affect were also indicated (Figure 3).Table 2

Bottom Line: TFs and miRNAs that DEGs significantly enriched were identified and a double-factor regulatory network was constructed.The DEGs were enriched for various pathways, with glucocorticoid receptor signaling as the most significant.A subnetwork under the regulation of MEF2C and SRF was also constructed to illustrate their regulatory effects on cardiac functions.

View Article: PubMed Central - PubMed

Affiliation: Department of Cardiology, Xi'an Children's Hospital, 69 Xi Ju Rd, Lianhu District, Xi'an, 710003, China. xawanglei@163.com.

ABSTRACT

Background: Cardiomyopathy is a progressive myocardial disorder. Here, we attempted to reveal the possible mechanism of cardiomyopathy at the transcription level with the roles of microRNAs (miRNAs) and transcription factors (TFs) taken into account.

Method: We firstly identified differentially expressed genes (DEGs) between cardiomyopathy patients and controls with data from the gene expression omnibus (GEO) database. DEGs were associated with the canonical pathways, molecular and cellular functions, physiological system development and function in the Ingenuity Knowledge Base by using the Ingenuity Pathway Analysis (IPA) software. TFs and miRNAs that DEGs significantly enriched were identified and a double-factor regulatory network was constructed.

Results: A total of 1,680 DEGs were identified. The DEGs were enriched for various pathways, with glucocorticoid receptor signaling as the most significant. A double-factor regulatory network was constructed, including seven TFs and two miRNAs. A subnetwork under the regulation of MEF2C and SRF was also constructed to illustrate their regulatory effects on cardiac functions.

Conclusion: Our results may provide new understanding of cardiomyopathy and may facilitate further therapeutic studies.

Show MeSH
Related in: MedlinePlus